Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Involvement of the Phospholipase C β1 Pathway in Desensitization of the Carbachol-activated Nonselective Cationic Current in Murine Gastric Myocytes

  • Kim, Byung Joo (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • So, Insuk (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
  • Kim, Ki Whan (Department of Physiology and Biophysics, Seoul National University College of Medicine)
  • Received : 2006.03.10
  • Accepted : 2006.05.23
  • Published : 2006.08.31
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Abstract

In murine gastrointestinal myocytes muscarinic stimulation activates nonselective cation channels via a G-protein and $Ca^{2+}$-dependent pathway. We recorded inward cationic currents following application of carbachol ($I_{CCh}$) to murine gastric myocytes held at -60 mV, using the whole-cell patch-clamp method. The properties of the inward cationic currents were similar to those of the nonselective cation channels activated by muscarinic stimulation in other gastrointestinal smooth muscle cells. CCh-induced $I_{CCh}$ and spontaneous decay of $I_{CCh}$ (desensitization of $I_{CCh}$) occurred. Unlike the situation in guinea pig gastric myocytes, desensitization was not affected by varying $[EGTA]_i$. Pretreatment with the PLC inhibitor (U73122) blocked the activation of $I_{CCh}$, and desensitization of $I_{CCh}$ was attenuated in PLC ${\beta}_1$ knock-out mice. These results suggest that the desensitization of $I_{CCh}$ in murine gastric myocytes is not due to a pathway dependent on intracellular $Ca^{2+}$ but to the PLC ${\beta}_1$ pathway.

Keywords

References

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