Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Induction of Immunity Against Hepatitis B Virus Surface Antigen by Intranasal DNA Vaccination Using a Cationic Emulsion as a Mucosal Gene Carrier

  • Kim, Tae Woo (Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University) ;
  • Chung, Hesson (Biomedical Research Center, Korea Institute of Science and Technology) ;
  • Kwon, Ick Chan (Biomedical Research Center, Korea Institute of Science and Technology) ;
  • Sung, Ha Chin (Graduate School of Biotechnology, Korea University) ;
  • Kang, Tae Heung (Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University) ;
  • Han, Hee Dong (Laboratory of Infection and Immunology, Graduate School of Medicine, Korea University) ;
  • Jeong, Seo Young (College of Pharmacy, Kyung Hee University)
  • Received : 2006.05.04
  • Accepted : 2006.08.16
  • Published : 2006.10.31
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Abstract

Delivery of DNA vaccines to airway mucosa would be an ideal method for mucosal immunization. However, there have been few reports of a suitable gene delivery system. In this study we used a cationic emulsion to immunize mice via the intranasal route with pCMV-S coding for Hepatitis B virus surface antigen (HBsAg). Complexing pCMV-S with a cationic emulsion dramatically enhanced HBsAg expression in both nasal tissue and lung, and was associated with increases in the levels of HBs-specific Abs in serum and mucosal fluids, of cytotoxic T lymphocytes (CTL) in the spleen and cervical and iliac lymph nodes, and of delayed-type hypersensitivity (DTH) against HBsAg. In contrast, very weak humoral and cellular immunities were observed following immunization with naked DNA. In support of these observations, a higher proliferative response of spleenocytes was detected in the group immunized with the emulsion/pCMV-S complex than in the group immunized with naked pCMV-S. These findings may facilitate development of an emulsion-mediated gene vaccination technique for use against intracellular pathogens that invade mucosal surfaces.

Keywords

Acknowledgement

Supported by : Kyung Hee University, KOSEF/MOST

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