Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Molecular and Structural Characterization of the Domain 2 of Hepatitis C Virus Non-structural Protein 5A

  • Liang, Yu (Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University) ;
  • Kang, Cong Bao (Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University) ;
  • Yoon, Ho Sup (Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University)
  • Received : 2006.02.02
  • Accepted : 2006.05.29
  • Published : 2006.08.31
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Abstract

Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional structure of the domain 1 was determined. However, currently the molecular basis for the domains 2 and 3 of HCV NS5A is yet to be defined. Toward this end, we expressed, purified the domain 2 of the NS5A (NS5A-D2), and then performed biochemical and structural studies. The purified domain 2 was active and was able to bind NS5B and PKR, biological partners of NS5A. The results from gel filtration, CD analysis, 1D $^1H$ NMR and 2D $^1H-^{15}N$ heteronuclear single quantum correlation (HSQC) spectroscopy indicate that the domain 2 of NS5A appears to be flexible and disordered.

Keywords

References

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