Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Cellular Changes Resulting from Forced Expression of Glypican-3 in Hepatocellular Carcinoma Cells

  • Kwack, Mi Hee (Department of Immunology, School of Medicine, Kyungpook National University) ;
  • Choi, Byung Yeol (Department of Immunology, School of Medicine, Kyungpook National University) ;
  • Sung, Young Kwan (Department of Immunology, School of Medicine, Kyungpook National University)
  • Received : 2005.11.01
  • Accepted : 2006.02.27
  • Published : 2006.04.30
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Abstract

Glypican-3 (GPC3) is a member of the glypican family, which encodes cell-surface heparan-sulfate proteoglycans, and is frequently upregulated in hepatocellular carcinoma (HCC). We have recently reported that blocking endogenous GPC3 expression promotes the growth of HCC cell lines, suggesting that GPC3 plays a negative role in HCC cell proliferation. Here, we report that forced expression of GPC3 reduced the growth of HCC cells. We also found that FGF2-mediated cell proliferation was inhibited by GPC3. In addition, we observed that the adhesion of HCC cells to collagen type I and fibronectin was decreased by GPC3, whereas cellular migration and invasiveness were stimulated. Collectively, these results suggest that progression of hepatocellular carcinoma is associated with upregulation of GPC3.

Keywords

Acknowledgement

Supported by : Kyungpook National University Hospital

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