Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Effects of Adipose Derived Stem Cells on Neurogenic Differentiation and Induction of Nerve Regeneration

인체 지방조직에서 유래한 줄기세포의 신경세포 분화능 및 신경재생 유도효과

  • Jun, Young Joon (Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, College of Medicine) ;
  • Rhie, Jong Won (Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, College of Medicine) ;
  • Choi, Yun Seok (Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, College of Medicine) ;
  • Kim, Young Jin (Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, College of Medicine) ;
  • Kim, Sung Eun (Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, College of Medicine) ;
  • Lee, Jong In (Department of Rehabilitation Medicine, The Catholic University of Korea) ;
  • Han, Ki Taik (Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, College of Medicine)
  • 전영준 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 이종원 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 최윤석 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 김영진 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 김성은 (가톨릭대학교 의과대학 성형외과학교실) ;
  • 이종인 (가톨릭대학교 의과대학 재활의학과교실) ;
  • 한기택 (가톨릭대학교 의과대학 성형외과학교실)
  • Received : 2005.11.25
  • Published : 2006.03.10
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Abstract

Using adipose derived stem cells(ASCs), neurogenic differentiation was induced in a mono layered culture medium containing neuronal induction agents. Cells differentiated to the neuronal cells were observed with a inverted microscope and immunofluorecent study. We made a 15 mm long defect in the sciatic nerve of 14 rats and connected a silicone tube to the defect. Then, we mixed neuronal progenitor cells differentiated from ASCs with collagen gel and grafted them to a group of rats(experimental group) and grafted only collagen gel into another group(control group). In 4 and 8 weeks after the graft, histological observation was made. According to the result, the number and diameter of myelinated axons were significantly increased in the experimental group. In addition, the nerve conduction velocity was improved more in the experimental group and neovascularity also increased. Moreover, reaction with S100 and p75 was observed in regenerated nerves in the experimental group, suggesting that the grafted cells were differentiated into supportive cells such as Schwann's cells. In conclusion, this research proved that ASCs can multiply and differentiate into neuronal cells. If they are grafted into nerve defects, the grafted cells are differ entiated into supportive cells such as Schwann's cells and thus contribute to nerve regeneration. Accordingly, the use of adipose tissue obtained easily without the limitation of donor site can be greatly helpful in treating peripheral nerve defects.

Keywords

References

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