Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
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Genotype of rotavirus isolated from patients with rotaviral enteritis and neurological complications

신경학적 이상이 동반된 로타바이러스 위장관염 환아에서의 로타바이러스 유전형

  • Choi, Jae Hyung (Department of Pediatrics, Hospital of Han Yang University) ;
  • Kim, Jeong-Mi (Department of Pediatrics, Hospital of Han Yang University) ;
  • Kim, Yong Joo (Department of Pediatrics, Hospital of Han Yang University) ;
  • Oh, Jae Won (Department of Pediatrics, Hospital of Han Yang University) ;
  • Kim, Chang Lyul (Department of Pediatrics, Hospital of Han Yang University) ;
  • Yum, Myung Kul (Department of Pediatrics, Hospital of Han Yang University) ;
  • Sul, In Joon (Department of Pediatrics, Hospital of Han Yang University) ;
  • Kang, Jung Oak (Department of Laboratory Medicine, Hospital of Han Yang University)
  • 최재형 (한양대학교 의과대학 소아과학교실) ;
  • 김정미 (한양대학교 의과대학 소아과학교실) ;
  • 김용주 (한양대학교 의과대학 소아과학교실) ;
  • 오재원 (한양대학교 의과대학 소아과학교실) ;
  • 김창렬 (한양대학교 의과대학 소아과학교실) ;
  • 염명걸 (한양대학교 의과대학 소아과학교실) ;
  • 설인준 (한양대학교 의과대학 소아과학교실) ;
  • 강정옥 (한양대학교 의과대학 소아과학교실, 임상병리학교실)
  • Received : 2005.11.04
  • Accepted : 2006.01.12
  • Published : 2006.05.15
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Abstract

Purpose : This study was undertaken to determine the differences in genotypes of rotavirus and their incidence between patients with acute rotaviral enteritis who suffered neurologic complications and those who did not suffer neurologic complications. Methods : Among the 82 patients with rotaviral enteritis whose genotype was analyzed, 71 patients were not associated with neurologic complications(neurology(-) group), and eleven patients were associated with neurologic complications(neurology(+) group). Four G genotypes and four P genotypes were determined by reverse trans cription and multiplex polymerase chain reaction. Results : In the neurology(-) group, the number of G4, G3, G2 and G1 was 24(35.3 percent), 23 (33.8 percent), 17(25.0 percent) and 4(5.6 percent), respectively and the number of P6, P4, P8 and P9 was 26(36.1 percent), 23(31.9 percent), 22(30.6 percent) and 1(1.4 percent), respectively. G2P4, and G4P6 were the most frequently found com bination genotypes, respectively. In the neurology(+) group, the number of G2, G3 and G4 was 9(75.0 percent), 2(16.7 percent) and 1(8.3 percent), respectively and the number of P4, P6, P8 and P9 was 8(66.7 percent), 2(16.7 percent), 1(8.3 percent) and 1(8.3 percent), respectively. G2P4 was the most frequently found combination genotype. The incidence of each of the G2, P4 and G2P4 was significantly higher in the neurology(+) group when compared to that of each of the G2, P4, and G2P4 in the neurology(-) group. Conclusion : In the patients with acute rotavirus gastroenteritis associated with neurologic complications, the G1 genotype was not found and genotypes of the G2, P4, and G2P4 were more frequently found.

목 적 : 본 연구에서는 로타바이러스 위장관염 환아에서 신경학적 이상이 동반된 경우와 그렇지 않은 경우를 비교하여 로타 바이러스 유전형의 분포와 빈도를 조사하고자 하였다. 방 법 : 로타바이러스 위장관염 환아에서 로타바이러스 유전형을 밝힌 82례 중 71례는 신경학적 이상이 동반되지 않았으며 11례에서 신경학적 이상이 동반되었다. 4군의 G형과 4군의 P형이 reverse transcription, multiplex polymerase chain reaction을 통해 분석되었다. 결 과 : 신경학적 이상이 동반되지 않은 군에서는 G4, G3, G2, G1형이 각각 24(35.3%), 23(33.8%), 17(25.0%), 4(5.6%)로, P6, P4, P8, P9형이 각각 26(36.1%), 23(31.9%), 22(30.6%), 1(1.4%)로 보고되었다. 또한 조합형으로 G2P4, G4P6이 가장 흔하게 관찰되었다. 신경학적 이상이 동반된 군에서는 G2, G3, G4형이 각각 9(75.0%), 2(16.7%), 1(8.3%)로, P4, P6, P8, P9형이 각각 8(66.7%), 2(16.7%), 1(8.3%), 1(8.3%)로 보고되었다. 또한 조합형으로 G2P4가 가장 흔하게 관찰되었다. 신경학적 이상이 있는 군에서 G2, P4, G2P4의 빈도는 신경학적 이상이 없는 군에 비해 그 빈도가 의미있게 높았다. 결 론 : 로타바이러스 위장관염 환아에서 신경학적 이상이 동반된 경우, 신경학적 이상이 동반되지 않은 경우에 비교하여 G1형이 관찰되지 않았으며, G2, P4, G2P4형이 더 흔하게 관찰되었다.

Keywords

References

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