Molecules and Cells

  • 제21권1호
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  • Pages.76-81
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  • 2006
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Quantitative Evaluation of the Mode of Microtubule Transport in Xenopus Neurons

  • Kim, Taeyong (Department of Life Science, Gwangju Institute of Science and Technology) ;
  • Chang, Sunghoe (Department of Life Science, Gwangju Institute of Science and Technology)
  • 투고 : 2005.08.17
  • 심사 : 2005.11.25
  • 발행 : 2006.02.28
⟨ 이전 논문 다음 논문 ⟩

초록

Tubulin is synthesized in the cell body and must be delivered to the axon to support axonal growth. However, the exact form in which these proteins, in particular tubulin, move within the axon remains contentious. According to the "polymer transport model", tubulin is transported in the form of microtubules. In an alternative hypothesis, the "short oligomer transport model", tubulin is added to existing, stationary microtubules along the axon. In this study, we measured the translocation of microtubule plus ends in soma segments, the middle of axonal shafts and the growth cone areas, by expressing GFP-EB3 in cultured Xenopus embryonic spinal neurons. We found that none of the microtubules in the three compartments were transported rapidly as would be expected from the polymer transport model. These results suggest that microtubules are stationary in most segments of the axon, thus supporting the model according to which tubulin is transported in nonpolymeric form in rapidly growing Xenopus neurons.

키워드

과제정보

연구 과제 주관 기관 : Ministry of Science and Technology

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