Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Satellite Cells Isolated from Adult Hanwoo Muscle Can Proliferate and Differentiate into Myoblasts and Adipose-like Cells

  • Kook, Sung-Ho (Institute of Oral Biosciences, Chonbuk National University) ;
  • Choi, Ki-Choon (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Korea University) ;
  • Son, Young-Ok (Institute of Oral Biosciences, Chonbuk National University) ;
  • Lee, Kyung-Yeol (Institute of Oral Biosciences, Chonbuk National University) ;
  • Hwang, In-Ho (Department of Animal Resources and Biotechnology, Chonbuk National University) ;
  • Lee, Hyun-Jeong (National Livestock Research Institute, Rural Development Administration) ;
  • Chang, Jong-Soo (Department of Agricultural Science, Korea National Open University,) ;
  • Choi, In-Ho (School of Biotechnology, Yeungnam University) ;
  • Lee, Jeong-Chae (Institute of Oral Biosciences, Chonbuk National University)
  • Published : 2006.10.31
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Abstract

This study examined whether adult bovine muscle satellite cells from 30-month-old Hanwoo cattle are multipotential. The satellite cells were found to have the potential to proliferate and differentiate into myoblasts with the formation of multinucleated cells. In addition, treatment with the peroxisome proliferator activating receptor-${\gamma}$ ($PPAR{\gamma}$) agonist, rosiglitazone, promoted their trans-differentiation into adipocytes with significant increases in glycerol accumulation and glycerol-3-phosphate dehydrogenase activity. Western blot analysis revealed that increased levels of the adipocyte fatty acid-binding protein, $PPAR{\gamma}$ and of CCAAT/enhancerbinding protein were closely related to rosiglitazoneinduced differentiation of the cells. These findings demonstrate that satellite cells from adult Hanwoo cattle are multipotent, and that their trans-differentiation into adipocytes can be induced by rosiglitazone.

Keywords

Acknowledgement

Supported by : RDA

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