한국어류학회지 (Korean Journal of Ichthyology)

  • 제18권1호
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  • Pages.12-19
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  • 2006
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  • 1225-8598(pISSN)
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  • 2288-3371(eISSN)
한국어류학회 (The Ichthyological Society of Korea)
권호 표지

미토콘드리아 cytochrome b 유전자 염기서열 분석에 의한 극동지역 송사리의 계통과 지리적 분포의 상관관계

Molecular Phylogeny and Distribution of Far Eastern Oryzias latipes Based on Mitochondrial Cytochrome b Gene Sequence

  • 어재영 (한국생명공학연구원 유전체정보센터) ;
  • 유정하 (경원대학교 화학생명공학과) ;
  • 강태욱 (한국생명공학연구원 유전체정보센터) ;
  • 김무상 ;
  • 김창배 (한국생명공학연구원 유전체정보센터)
  • Eah, Jae-Yong (National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Jeong-Ha (Department of Chemical & Bioengineering, Kyungwon University) ;
  • Kang, Tae-Wook (National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Moo-Sang (Department of Molecular Cellular and Developmental Biology, Yale University) ;
  • Kim, Chang-Bae (National Genome Information Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2005.12.27
  • 심사 : 2006.03.06
  • 발행 : 2006.03.31
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초록

본 연구에서는 극동지역 Oryzias latipes의 지리적 분포와 미토콘드리아 cytochrome b (cyt b)의 유전적 다양성과의 관계를 밝히기 위해 전국 9개의 지역에서 53개체를 채집하여 얻은 cyt b 염기서열과 GenBank에 등록되어있던 117개의 데이터를 종합하여 이를 142개의 haplotype으로 새로이 변환하였고, 이의 계통수를 작성 분석하였다. 분석 결과 극동지역 송사리는 3개의 haplogroup (A, B, C)으로 나뉘어지며, haplogroup A는 남한의 남한아지역을 중심으로 남한 전역에 분포하며, haplogroup B는 중국와 남한의 서한아지역에 걸쳐 분포하며, haplogroup C는 일본에만 분포하였다. Haplogroup A는 haplogroup B와 한반도내에서 지리적으로 가깝게 분포하고 있으나 계통적으로는 상당한 거리를 가지고 뚜렷하게 구별되었다. haplotype의 분지양상은 선행된 연구결과와 거의 일치하였다.

To examine the relationship of geological distribution and phylogenetic tree of O. latipes in the Far East, we analyzed cytochrome b (cyt b) gene in the mitochondrial genome. In this study we employed the entire sequence of cyt b of 53 samples collected from nine Korean locations and 117 cyt b data retrieved from the GenBank. From 170 Oryzias latipes cyt b sequence data, 142 different haplotypes were identified and phylogenetic relationship was reconstructed based on the dataset. According to the phylogeny, haplotypes were divided into three major haplogroups A, B and C, and their relationships were well correlated to their distributional patterns. Haplogroup A which is widely distribute in the southern part of Korea is separated in the geographical distribution from the haplogroup B which is found from China to the western part of Korea. Haplogroup C is only found in Japan.

키워드

과제정보

연구 과제 주관 기관 : 환경부, 한국생명공학연구원

참고문헌

  1. Cantatore, P., M. Roberti, G. Pesole, A. Ludovico, F. Milella, M.M. Gadaleta and C. Saccone. 1994. Evolutionary analysis of cytochrome b sequences in some Perciformes: evidence for a slower rate of evolution than in mammals. J. Mol. Evol., 39 : 589-597 https://doi.org/10.1007/BF00160404
  2. Hall, T. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser., 41 : 95-98
  3. Harada, S., S.R. Joen, I. Kinoshita, M. Tanaka and M. Nishida. 2002. Phylogenetic relationships of four species of floating gobies (Gymnogobius) as inferred from partial mitochondrial cytochrome b gene sequences. Ichtyol. Res., 49 : 324-332 https://doi.org/10.1007/s102280200048
  4. Hardman, M. 2005. The phylogenetic relationships among non-diplomystid catfishes as inferred from mitochondrial cytochrome b sequences; the search for the ictalurid sister taxon (Otophysi: Siluriformes). Mol. Phylogenet. Evol., 37 : 700-720 https://doi.org/10.1016/j.ympev.2005.04.029
  5. Kang, T.W., E.H. Lee, M.S. Kim, S.G. Paik, S. Kim and C.B. Kim. 2005. Molecular phylogeny and geography of Korean medaka fish (Oryzias latipes). Mol. Cells., 20 : 151-156
  6. Kim, I.S. and S.Y. Kim. 1993. Interspecific hybridization between Oryzias sinensis and O. latipes from Korea. Korean J. Ichthyol., 5 : 113-121
  7. Kim, I.S. and E.H. Lee. 1992. New records of rice fish, Oryzias latipes sinensis (Pisces, Oryzidae) from Korea. Korean J. Syst. Zool., 8 : 177-182
  8. Kim, I.S. and K.C. Moon. 1987 The karyotype of a rice fish, Oryzias latipes from southern Korea. Korean J. Zool., 30 : 379-386
  9. Kumar, S., K. Tamura and M. Nei. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. in Bioinform., 5 : 150-163 https://doi.org/10.1093/bib/5.2.150
  10. Matsuda, M., T. Yamagishi, M. Sakaizumi and S.R. Jeon. 1997. Mitochondrial DNA variation in the Korean wild population of medaka, Oryzias latipes. Korean J. Limnol., 30 : 119-128
  11. Orti, G., M.A. Bell, T.E. Reimchen and A. Meyer. 1994. Global survey of mitochondrial DNA sequences in the threespine stickleback; evidence for recent migrations. Evolution, 48 : 608-622 https://doi.org/10.2307/2410473
  12. Pepe, T., M. Trotta, I. di Marco, P. Cennamo, A. Anastasio and M.L. Cortesi. 2005. Mitochondrial cytochrome b DNA sequence variations: an approach to fish species identification in processed fish products. J. Food Prot., 68: 421-425 https://doi.org/10.4315/0362-028X-68.2.421
  13. Rocha-Olivares, A., R.H. Rosenblatt and R.D. Vetter. 1999. Molecular evolution, systematics, and zoogeography of the rockfish subgenus Sebastomus (Sebastes, Scorpaenidae) based on mitochondrial cytochrome b and control region sequences. Mol. Phylogenet Evol., 11: 441-458 https://doi.org/10.1006/mpev.1998.0585
  14. Rozas, J. and R. Rozas. 1999. DnaSP version 3: an integrated program for molecular population genetics and molecular evolution analysis. Bioinformatics, 15: 174 -175 https://doi.org/10.1093/bioinformatics/15.2.174
  15. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4 : 406-425
  16. Sakaizumi, M. and S.R. Jeon. 1987. Two divergent groups in the wild populations of medaka Oizias latipes (Pisces; Oryzitidae) in Korea. Korean J. Lim., 20 : 13-20
  17. Takehana, Y., N. Nagai, M. Matsuda, K. Tsuchiya and M. Sakaizumi. 2003. Geographic variaition and diversity of the cytochrome b gene in Japanese wild populations of medaka, Oryzias latipes. Zoolog. Sci., 20 : 1279-1291 https://doi.org/10.2108/zsj.20.1279
  18. Takehana, Y., N. Nagai, M. Matsuda, K. Tsuchiya and M. Sakaizumi. 2004. Geographic variation and diversity of the cytochrome b gene in wild populations of medaka (Oryzias latipes) from Korea and China. Zoolog. Sci., 21 : 483-491 https://doi.org/10.2108/zsj.21.483
  19. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 25 : 4876-4882 https://doi.org/10.1093/nar/25.24.4876
  20. Uwa, H. and S.R. Jeon. 1987. Karyotypes in two divergent groups of a ricefish, Oryzias latipes, from Korea. Korean J. Lim., 20 : 139-147
  21. 김익수.최 윤.이충렬.이용주.김병직.김지현. 2005. 원색 한국어류대도감. 교학사, pp. 1-616