Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Establishment and Characterization of Three Immortal Bovine Muscular Epithelial Cell Lines

  • Jin, Xun (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Lee, Joong-Seob (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Kwak, Sungwook (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Lee, Soo-Yeon (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Jung, Ji-Eun (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Kim, Tae-Kyung (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Xu, Chenxiong (The Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Hong, Zhongshan (The Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Li, Zhehu (The Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Sun-Myung (The Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Pian, Xumin (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Lee, Dong-Hee (Department of Life Science, University of Seoul) ;
  • Yoon, Jong-Taek (Department of Animal Life and Resources, Hankyung National University) ;
  • You, Seungkwon (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University) ;
  • Choi, Yun-Jaie (The Laboratory of Animal Cell Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Hyunggee (The Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University)
  • Received : 2005.07.20
  • Accepted : 2005.10.07
  • Published : 2006.02.28
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Abstract

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack $p16^{INK4a}$ regulatory function, compared to primary BME cells that were growth arrested by enforced expression of $p16^{INK4a}$. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

Keywords

Acknowledgement

Supported by : Korea Ministry of Education

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