Antioxidative Effect of Crude Anthocyanins in Water-in-Oil Microemulsion System

  • Oh, Ju-Kyoung (Department of Food and Nutrition, Kookmin University) ;
  • Kim, Seok-Joong (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Imm, Jee-Young (Department of Food and Nutrition, Kookmin University)
  • Published : 2006.04.30

Abstract

Antioxidative ability of anthocyanins in water-in-oil microemulsion was examined. Microemulsion was prepared by solubilizing crude anthocyanins extracted from grape skin (Cambell early) in organic solvent (hexane) containing anionic surfactant [bis (2-ethylhexyl) sodium sulfosuccinate, AOT] and linolenic acid (10%, w/v). Lipid oxidation significantly decreased with increasing concentration of anthocyanins ($5-20\;{\mu}M$) at micellar phase, and increasing micelle size ($Wo=5-20\;{\mu}M$). At given micelle size (Wo=10), lipid oxidation decreased as number of micelles decreased. These results indicate antioxidative ability of anthocyanins is critically affected by water core and micelle structure formed by surfactant. Interactions between AOT and anthocyanins decreased antioxidative ability of anthocyanins. Antioxidative ability of anthocyanins significantly increased when ${\alpha}$-tocopherol was added into organic phase. This indicates of synergism between the two antioxidants.

Keywords

References

  1. Min B, Ahn DU. Mechanism of lipid peroxidation in meat and meat products- a review. Food Sci. Biotechnol. 14: 152-163 (2005)
  2. Halliwell B, Murcia MA, Chirico S, Aruoma OI. Free radicals and antioxidants in food and in vivo: What they do and how they work. Crit. Rev. Food Sci. Nutr. 35: 7-20 (1995)
  3. Lee EJ, Kwon YI, Shetty K, Jang HD. Antioxidant activity of Rhodiola rosea extract on human low-density lipoprotein oxidation and DNA strand scission. Food Sci. Biotechnol. 13: 814-820 (2004)
  4. Neadis N, Zafiropoulou I, Tsimidou M. Commonly used food antioxidants: a comparative study in dispersed systems. Food Chem. 82: 403-407 (2003) https://doi.org/10.1016/S0308-8146(02)00579-4
  5. Mei L, McClements DJ, Wu J, Decker EA. Iron-catalyzed lipid oxidation in emulsion as affected by surfactant, pH and NaCI. Food Chem. 61: 307-312 (1998) https://doi.org/10.1016/S0308-8146(97)00058-7
  6. Silvestre MPC, Chaiyasit W, Brannan RD, McClements DJ, Decker EA. Ability of surfactant head group size to alter lipid and antioxidant oxidation in oil-in-water emulsions. J. Agric. Food Chem. 48: 2057-2061 (2000) https://doi.org/10.1021/jf991162l
  7. Luisi PL, Laane C. Solubilization of enzymes in apolar solvents via reverse micelles. Trends Biotechnol. 4: 153-161 (1986) https://doi.org/10.1016/0167-7799(86)90166-6
  8. Martinek K, Klyachko NL, Kabanov AV, Khmelnitsky YL, Levashov AY. Micellar enzymology: its relation to membranology. Biochem. Biophys. Acta 981: 161-172 (1989) https://doi.org/10.1016/0005-2736(89)90024-2
  9. Melo EP, Aires-Barros MR, Cabral JMS. Reverse micelles and protein biotechnology. Biotechnol. Ann. Rev. 7: 87-129 (2001) https://doi.org/10.1016/S1387-2656(01)07034-X
  10. Wrolstad RE. Anthocyanin pigments-bioactibity and coloring properties. J. Food Sci. 69: 419-425 (2004) https://doi.org/10.1111/j.1365-2621.2004.tb10709.x
  11. Wang H, Cao G, Prior RP. Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem. 45: 304-309 (1997) https://doi.org/10.1021/jf960421t
  12. Satue-Gracia MT, Heinonen M, Frankel EN. Anthocyanins as antioxidants on human low-density lipoprotein and lecithin-liposome systems. J. Agric. Food Chem. 45: 3362-3367 (1997) https://doi.org/10.1021/jf970234a
  13. Kim SJ, Cho YH, Park WS, Han DS, Chai CH, Imm JY. Solubilization of water soluble anthocyanins in apolar medium using reverse micelle. J. Agric. Food. Chem. 51: 7805-7809 (2003) https://doi.org/10.1021/jf034843w
  14. Skrede E, Wrolstad RE, Durst RW. Changes in anthocyanins and polyphenolics during juice processing of highbush blueberries (Vaccinium corymbosum L.). J. Food Sci. 65: 357-364 (2000) https://doi.org/10.1111/j.1365-2621.2000.tb16007.x
  15. AOCS. Official method of Analysis of AOCS Int. 4th ed. Method Cd 8-53. Official Methods and Recommended Practices of the American Oil Chemists' Society, AOCS Press, Champaign, IL, USA (1996)
  16. Zuluf R, Eicke HF. Inverted micelles and microemulsions in the ternary system $H_2O$/Aerosol-OT/isooctane as studied by photon correlation spectroscopy. J. Phys. Chem. 83: 480-486 (1979) https://doi.org/10.1021/j100467a011
  17. McClements DJ, Decker EA. Lipid oxidation in oil-in-water emulsions: impact of molecular environment on chemical reactions in heterogeneous food systems. J. Food Sci. 65: 1270-1282 (2000) https://doi.org/10.1111/j.1365-2621.2000.tb10596.x
  18. Luisi PL, Giomini M, Pileni MP, Robinson BH. Reverse micelles as hosts for proteins and small molecules. Biochim. Biophys. Acta 947: 209-246 (1988) https://doi.org/10.1016/0304-4157(88)90025-1
  19. Richards MP, Chaiyasit W, McClements DJ, Decker EA. Ability of surfactant micelles to alter the partitioning of phenolic antioxidants in oil-in-water emulsions. J. Agric. Food Chem. 50: 1254-1259 (2002) https://doi.org/10.1021/jf011324p
  20. Schwarz K, Frankel EN, German JB. Partition behavior of antioxidant phenolic compounds in heterophasic systems. Fett/Lipid 98: 115121 (1996) https://doi.org/10.1002/lipi.19960980306
  21. Cohen B, Huppert D, Solntsev KM, Tsfadia Y, Nachliel E, Gutman M. Excited state proton transfer in reverse micelles. J. Am. Chem.Soc. 124: 7539-7547 (2002) https://doi.org/10.1021/ja012646c
  22. Silber JJ, Biasutti A, Abuin E, Lissi E. Interactions of small molecules with reverse micelles. Adv. Colloid Interfac. 82: 189-252 (1999) https://doi.org/10.1016/S0001-8686(99)00018-4
  23. Mulinacci N, Romani A, Pinelli P, Gallori S, Giaccherini C, Vincieri FE Stabilization of natural anthocyanins by micellar systems. Int. J. Pharm. 216: 23-31 (2001) https://doi.org/10.1016/S0378-5173(00)00685-2
  24. Foti M, Ruberto G. Kinetic solvent effects on phenolic antioxidants determined by spectrophotometric measurements. J. Agric. Food Chem. 49: 342-348 (2001) https://doi.org/10.1021/jf0006527
  25. Decker EA. Strategies for manipulating the prooxidative/antioxidative balance of foods to maximize oxidative stability. Trends Food Sci. Technol. 9: 241-248 (1998) https://doi.org/10.1016/S0924-2244(98)00045-4
  26. Yi OS, Cho DW. Antioxidative effect of reverse micellar system containing ascorbic acid and -tocopherol. Food Biotechnol. 4: 128129 (1995)
  27. Han D, Yi OS, Shin HK. Antioxidative effect of ascorbic acid solubilized in oils via reversed micelles. J. Food Sci. 55: 247-251 (1990) https://doi.org/10.1111/j.1365-2621.1990.tb06062.x