Enhancement of Immunological Activities in Mice by Oral Administration of Pectic Polysaccharides from Eleutherococcus senticosus

  • Sung, Ji-Yun (Food Business R&D, Boryung Co., Ltd.) ;
  • Yoon, Taek-Joon (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University and Bio-Food and Drug Research Center) ;
  • Yu, Kwang-Won (Department of Kimchi and Food Science, Chungju National College of Science and Technology) ;
  • Lee, Kwang-Ho (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University and Bio-Food and Drug Research Center) ;
  • Lee, Ho (Department of Food Science and Biotechnology, Kyonggi University)
  • Published : 2006.02.28

Abstract

Ability of pectic polysaccharides isolated from Eleutherococcus senticosus EN-3 to inhibit tumor metastasis and induce antigen-specific immune response after oral administration in mice was assessed. Consecutive oral administration of EN-3 before tumor inoculation dramatically inhibited tumor metastasis produced by colon26-M3.1 and B16-BL6 cells. When Peyer's patch cells isolated from mouse intestine were co-cultured with EN-3, proliferation of Peyer's patch cells was induced. Mice co-administered with EN-3 and ovalbumin (OVA) showed significantly higher production of OVA-specific IgA in intestinal washing as well as IgG in serum than those administered with OVA alone. Payer's patch cells of mice immunized with OVA plus EN-3 showed much higher proliferating activity than those of mice immunized with OVA alone. Proliferating activity increased dose-dependently, indicating EN-3 specifically enhanced mucosal immune response to OVA. These results suggested EN-3 could significantly stimulate Peyer's patch cells either non-specifically or antigen-specifically, possibly playing important role in enhancement of mucosal and systemic immune systems.

Keywords

References

  1. Shin, SM, Hong, ST. Acanthopanax and Platycodi independently prevents the onset of high fat diet induced hyperg1yceridemia and obesity in C57BL/6 mice. Food Sci. Biotechnol. 14: 841-846 (2005)
  2. Davydov M, Krikorian, AD. Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as an adaptogen: a closer look. J. Etlmopharmacol. 72: 345-393 (2000) https://doi.org/10.1016/S0378-8741(00)00181-1
  3. Schmeda-Hirschmann G, Villasenor-Garcia MM, Lozoya X. PueblaPerez, AM. Immunomodulatory activity of Chilean Cyttaria species in mice with L5178Y lymphoma. J. Ethnopharmacol. 77: 253-257 (2001) https://doi.org/10.1016/S0378-8741(01)00284-7
  4. Schmolz MW, Sacher F, Aicher, B. The synthesis of Rantes, G-CSF, IL-4, IL-5, IL-6, IL-12 and IL-13 in human whole-blood cultures is modulated by an extract from Eleutherococcus senticosus L. roots. Phytother. Res. 15: 268-270 (2001) https://doi.org/10.1002/ptr.746
  5. Jeong, HJ, Koo HN, Myung, NI, Shin MK, Kim JW, Kim DK, Kim KS, Kim HM, Lee YM. Inhibitory effects of mast cell-mediated allergic reactions by cell cultured Siberian Ginseng. Immunopharmacol. Immunotoxicol. 23: 107-117 (2001)
  6. Yi 1M, Kim MS, Sea SW, Lee KN, Yook CS, Kim HM. Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis. Clin. Chim. Acta 312: 163-168 (2001) https://doi.org/10.1016/S0009-8981(01)00613-1
  7. Umeyama A, Shoji N, Takei M, Endo K, Arihara S. Ciwujianosides Dl and Cl: powerful inhibitors of histamine release induced by anti-immunoglobulin E from rat peritoneal mast cells. J. Pharmacol. Sci. 81: 661-662 (1992) https://doi.org/10.1002/jps.2600810714
  8. Holmgren J, Czerkinsky C, Eriksson K, Mharandi A. Mucosal immunisation and adjuvants: a brief overview of recent advances and challenges. Vaccine 21 Suppl 2: S89-95 (2003) https://doi.org/10.1016/S0264-410X(03)00206-8
  9. Dechant M, Valerius T. 19A antibodies for cancer therapy. Crit. Rev. Oncol. Hemat. 39: 69-77 (2001) https://doi.org/10.1016/S1040-8428(01)00105-6
  10. Holmgren J, Czerkinsky C. Mucosal immunity and vaccines. Nat. Med. 11: 45-53 (2005) https://doi.org/10.1038/nm1213
  11. Israf DA, Lajis NH, Somchit MN, Sulaiman MR. Enhancement of ovalbumin-specific IgA responses via oral boosting with antigen coadministered with an aqueous Solanum torvum extract. Life Sci. 75: 397-406 (2004) https://doi.org/10.1016/j.lfs.2003.10.038
  12. Hwang SH, Ha ES, Yu KW, Shin KS, Lee SH, Lee JK, Lee KH, Yoon TJ, Park WM. Effect of crude polysaccharides fraction from Eleutheroccus senticosus as a immunoadjuvant to soluble antigen (BSA and OVA). Yakhak Hoiji 47: 167-175 (2003)
  13. Ha ES, Hwang SH, Shin KS, Yu KW, Lee KH, Choi JS, Park WM, Yoon TJ. Anti-metastatic activity of glycoprotein fractionated from Acanthopanax senticosus, involvement o fNK-cell and macrophage activation. Arch. Pharm. Res. 27: 217-224 (2004) https://doi.org/10.1007/BF02980109
  14. Yoon TJ, Yoo YC, Lee SW, Shin KS, Choi WH, Hwang SH, Ha ES, Jo SK, Kim SH, Park WM. Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action. J. Ethnopharmacol. 93: 247-253 (2004) https://doi.org/10.1016/j.jep.2004.03.052
  15. Yoon TJ, Lee SH, Hwang SH, Shin KS, Park WM, Hwang JH, Yu KW. Immunomodulating activity of pectic polysaccharides from Eleutherococcus senticosus. Food Sci. Biotechnol. 12: 533-539 (2003)
  16. Iigo M, Kuhara T, Ushida Y, Sekine K, Moore MA, Tsuda U. Inhibitory effects of bovine lactoferrin on colon carcinoma 26 lung metastasis in mice. Clin. Exp. Metastasis 17: 35-40 (1999)
  17. Wang D, Kandimalla ER, Yu D, Tang JX, Agrawal S. Oral administration of second-generation immunomodulatory oligonucleotides induces mucosal Thl immune responses and adjuvant activity. Vaccine 23: 2614-2622 (2005) https://doi.org/10.1016/j.vaccine.2004.11.028
  18. McGhee JR, Mestecky J, Dertzbaugh MT, Eldridge, JH, Hirasawa M, Kiyono H. The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10: 75-88 (1992) https://doi.org/10.1016/0264-410X(92)90021-B
  19. Yoon TJ, Yoo YC, Kang TB, Baek YJ, Huh CS, Song SK, Lee KH, Azuma I, Kim JB. Prophylactic effect of Korean mistletoe (Viscum album colora tum ) extract on tumor metastasis is mediated by enhancement of NK cell activity. Int. J. Immunopharmacol. 20: 163-172 (1998) https://doi.org/10.1016/S0192-0561(98)00024-1
  20. Yoo YC, Saiki I, Sato K, Azuma 1. MDP-Lys(Ll8), a lipophilic derivative of muramyl dipeptide, inhibits the metastasis of haematogenous and non-haematogenous tumours in mice. Vaccine 12: 175-176 (1994) https://doi.org/10.1016/0264-410X(94)90057-4
  21. Gazitt Y. Immunologic profiles of effector cells and peripheral blood stem cells mobilized with different hematopoietic growth factors. Stem Cells 18: 390-398 (2000) https://doi.org/10.1634/stemcells.18-6-390
  22. Jun WJ, Kim SH, Lee DH, Chung JW, Sim SG, Lee KG, Cho HY, Hong BS. In vitro and in vivo effects of Gelidium amansii on intestinal immune system. Food Sci. Biotechnol. 14: 147-151 (2005)
  23. Reudl C, Wolf W. Features of oral immunization. Int. Arch. Allergy Imm. 108: 334-339 (1995) https://doi.org/10.1159/000237177
  24. McCluskie MJ, Weeratna RD, Krieg AM, Davis HL. CpG DNA is an effective oral adjuvant to protein antigens in mice. Vaccine 19: 950-957 (2000) https://doi.org/10.1016/S0264-410X(00)00215-2