Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Estimation of Estrogenic Activity by E-screen Assay and Stepwise Analysis of Endocrine Disruptors in Pilot Sewage Treatment Plant

파일럿 규모의 하수 처리 공정별 E-screen Assay에 의한 에스트로겐 활성과 내분비계 장애물질의 농도 평가

  • Lee, Ji-Ho (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering) ;
  • Park, Jong-Yol (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering) ;
  • Ra, Jin-Sung (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering) ;
  • Duong, Cuong N. (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering) ;
  • Lee, Byoung-Cheun (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering) ;
  • Kim, Sang-Don (Gwangju Institute of Science and Technology, Department of Environmental Science and Engineering)
  • 이지호 (광주과학기술원 환경공학과) ;
  • 박종열 (광주과학기술원 환경공학과) ;
  • 나진성 (광주과학기술원 환경공학과) ;
  • ;
  • 이병천 (광주과학기술원 환경공학과) ;
  • 김상돈 (광주과학기술원 환경공학과)
  • Published : 2006.07.31
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Abstract

Endocrine disruptors were measured with GC/MS in effluents discharged from sewage treatment processes in pilot scale for the purpose of water reuse. From that analysis, we compared the removal rate of them by treatment processes. Nonylphenol was mainly detected in effluents and high concentration from 0.36 to 0.94 ${\mu}g/L$. $17{\beta}$-estradiol(E2) and $17{\alpha}$-ethynylestradiol(EE2) were detected as below the limit of detection in effluent. Endocrine disruptors were removed effectively in the range from 50 to 100% by treatment process. EC50 value($9.0{\times}10^{-3}$ M) of $17{\beta}$-estradiol(E2) by dose response curve of E-screen assay has higher than that of bisphenol A($2.736{\times}10^{-5}M$) and p-octylphenol($9.760{\times}10^{-6}$ M). These results showed that alkylphenols have lower relative estrogen potency than other estrogens such as $17{\beta}$-estradiol(E2). Calculated estrogenic activity(ng-EEQ/L) was 2 times higher than measured total estrogenic activity which estimated by E-screen assay. Moreover estrogenic activity of effluent by treatment process showed very low as below 1 ng-EEQ/L.

물 재이용 목적으로 설계한 pilot scale의 하수 처리공정에서 배출되는 방류수 중 내분비계 장애물질을 GC/MS로 분석하였고, 처리 공정별로 제거율을 비교하였다. 각 처리 공정별 방류수에서 nonylphenol이 주로 검출되었고, 평균 $0.36{\sim}0.94$ ${\mu}g/L$으로 높게 검출되었으나, E2와 EE2는 처리수에서 정량 이하로 검출되었다. 내분비계 장애물질은 처리 공정별로 $50{\sim}100%$의 제거율을 보여주었다. E-screen assay에 의해 얻어진 양-반응 곡선에서 E2의 EC50값은 $9.0{\times}10^{-3}$ M로 bisphenol A와 p-octylphenol의 EC50값인 $2.736{\times}10^{-5}$ M, $9.760{\times}10^{-6}$ M에 비해 매우 높았다. 이는 알킬페놀류가 E2에 대한 상대적인 에스트로겐 활성도가 매우 낮음을 보여주었다. 환경 호르몬 농도와 이 물질의 상대적인 에스트로겐 활성도에 근거하여 계산된 에스트로겐 활성도(ng-EEQ/L)는 E-screen assay에 의해 실측한 총 에스트로겐 활성도(ng-EEQ/L)에 비해 평균 2배의 높은 활성도를 보여 주었다. 각 처리 공정별 방류수의 에스트로겐 활성도는 1 ng-EEQ/L 이하의 매우 낮은 활성도를 보여주었다.

Keywords

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