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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus

  • Zhou, Liang (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Zhang, Jiamin (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Wang, Xiaochun (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Jiang, Hong (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Yi, Fuming (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Hu, Yuanyang (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
  • Received : 2006.02.19
  • Accepted : 2006.05.19
  • Published : 2006.09.30
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Abstract

Dendrolimus punctatus tetravirus (DpTV) has been identified as a new member of the genus Omegatetravirus of the family Tetraviridae that may be related serologically to Nudaurelia capensis virus ($N{\omega}V$). To establish the function of DpTV RNA genome and to better understand the mechanism of viral replication, the putative RNA-dependent RNA polymerase (RdRp) domain has been cloned and expressed in Escherichia coli. The recombinant protein was purified on a Ni-chelating HisTrap affinity column and demonstrated to initiate viral RNA synthesis in a primer-independent manner but not by terminal nucleotidyle transferase activity in the presence of $Mg^{2+}$ and RNA template. Mutation of the GDD to GAA interferes with the residues at the polymerase active site and metal ions, and thus renders the polymerase inactive.

Keywords

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