BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of Transmembrane Domain of a Membrane Associated Protein NS5 of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus

  • Chen, Wuguo (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Zhang, Jiamin (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Dong, Changjin (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Yang, Bo (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Li, Yanqiu (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Liu, Chuanfeng (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University) ;
  • Hu, Yuanyang (State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University)
  • Received : 2006.03.13
  • Accepted : 2006.04.11
  • Published : 2006.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

We examined the intracellular localization of NS5 protein of Dendrolimus punctatus cytoplasmic polyhedrosis virus (DpCPV) by expressing NS5-GFP fusion protein and proteins from deletion mutants of NS5 in baculovirus recombinant infected insect Spodoptera frugiperda (Sf-9) cells. It was found that the NS5 protein was present at the plasma membrane of the cells, and that the N-terminal portion of the protein played a key role in the localization. A transmembrane region was identified to be present in the N-terminal portion of the protein, and the detailed transmembrane domain (SQIHMVWVKSGLVFF, 57-71aa) of N-terminal portion of NS5 was further determined, which was accorded with the predicted results, these findings suggested that NS5 might have an important function in viral life cycle.

Keywords

References

  1. Akhtar, S., Meek, K. M. and James, V. (1999) Immunolocalization of elastin, collagen type I and type III, fibronctin, and vitronectin in extracellular matrix components of normal and myxomatous mitral heart valve chordae tendineae. Cardiovasc. Pathol. 8, 203-211
  2. Alexandra, S., Terry, G., Shujing, R., Susan, W., Gerry, C., Peter, P. C. M. and James, J. B. (2005) Morphological and Molecular Characterization of a Cypovirus (Reoviridae) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae). J. Virol. 79, 9430-9438 https://doi.org/10.1128/JVI.79.15.9430-9438.2005
  3. Arella, M., Lavallee, C., Belloncik, S. and Furuichi, Y. (1988) Molecular cloning and characterization of cytoplasmic polyhedrosis virus polyhedron and a viable deletion mutant gene. J. Virol. 62, 211-217
  4. Beaton, A. R., Rodriguez, J., Reddy, Y. K. and Roy, P. (2002) The membrane trafficking protein calpactin forms a complex with bluetongue virus protein NS3 and mediates virus. Proc. Natl. Acad. Sci. USA 99, 13154-13159 https://doi.org/10.1073/pnas.192432299
  5. Belloncik, S. (1989) Cytoplasmic polyhedrosis viruses-Reoviridae. Adv. Virus. Res. 37, 173-209 https://doi.org/10.1016/S0065-3527(08)60835-0
  6. Belloncik, S., Liu, J., Su, D. and Arella, M. (1996) Identification and characterization of a new cypovirus,type 14, islolated form Heliothis armigera. J. Invertebr. Pathol. 67, 41-47 https://doi.org/10.1006/jipa.1996.0007
  7. Belloncik, S. and Mori, H. (1998) Cypoviruses, in: The Insect Viruses, Miller, L. K., Ball, L. A. (eds.), pp. 337-369, Plenum, USA
  8. Cai, C. Z., Han, L. Y., Ji, Z. L., Chen, X. and Chen, Y. Z. (2003) SVM-Prot: web-based support vector machine software for functional classification of a protein from its primary sequence. Nucleic Acids Res. 31, 3692-3697 https://doi.org/10.1093/nar/gkg600
  9. Du, J. Y., Zhang, J. M., Guo, H. T. and Hu, Y. Y. (2001) Molecular cloning and sequence analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus polyhedron gene. Virol. Sin. 16, 350-354
  10. Elazar, M., Cheong, K. H., Liu, P., Greenberg, H. B., Rice, C. M. and Glenn, J. S. (2003) Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication. J. Virol. 77, 6055-6061 https://doi.org/10.1128/JVI.77.10.6055-6061.2003
  11. Furuichi, Y. and Miura, K. I. (1973a) Identity of the 3'-terminal sequences in ten genome segments of silkworm cytoplasmic polyhedrosis virus. Virol. 5, 418-425
  12. Furuichi, Y. and Miura, K. I. (1975b) A blocked structure at the 5'-terminus of mRNA of cytoplasmic polyhedrosis virus. Nature 253, 374-375 https://doi.org/10.1038/253374a0
  13. Gromiha, M. M., Ahmad, S. and Suwa, M. (2005) TMBETANET: discrimination and prediction of membrane spanning $\beta$-strands in outer membrane proteins. Nucleic Acids Res. 33, 164- 167
  14. Guo, Y. X., Chan, S. W. and Kwang, J. (2004) Membrane association of Greasy grouper nervous necrosis virus protein A and characterization of its mitochondrial localization targeting signal. J. Virol. 78, 6498-6508 https://doi.org/10.1128/JVI.78.12.6498-6508.2004
  15. Hassan, S. H., Wirblich, C., Forzan, M. and Roy, P. (2001) Expression and functional characterization of Bluetongue virus VP5 protien: role in cellular permeabilization. J. Virol. 75, 8356-8367 https://doi.org/10.1128/JVI.75.18.8356-8367.2001
  16. Hong, J. J., Duan, J. L., Zhao, S. L., Xu, H. G. and Peng, H. Y. (2004) Nucleotide sequences of genome segments S6, S7 and S10 of Dendrolimus punctatus cypovirus 1. Arch. Virol. 149, 183-190 https://doi.org/10.1007/s00705-003-0193-7
  17. Kuchino, Y., Nishimura, S., Smith, R. E. and Furuichi, Y. (1982) Homologous terminal sequences in the double-stranded RNA genome segments of cytoplasmic polyhedrosis virus of the silkworm Bombyx mori. J. Virol. 44, 538-543
  18. Mller, S., Croning, M. D. R. and Apweiler, R. (2001) Evaluation of methods for the prediction of membrane spanning regions. Bioinformatic. 17, 646-653 https://doi.org/10.1093/bioinformatics/17.7.646
  19. Newton, K., Meyer, J. C., Bellamy, A. R. and Taylor, J. A. (1997) Rotavirus nonstructural glycoprotein NSP4 alters plasma membrane permeability in mammalian cells. J. Virol. 71, 9458-9465
  20. Niekerk, M. V., Smit, C. C., Fick, W. C., Staden, V. V. and Huismans H (2001) Membrane association of African Horsesickness virus nonstructural protein NS3 determines its cytotoxicity. Virol. 279, 499-508 https://doi.org/10.1006/viro.2000.0709
  21. Paul, R. L., Susan, J. C., Owen, E. C. and Lan, N. C. (1992) Cloning of noncultivatable human rotavirus by single primer amplification. J. Virol. 66, 1817-1822
  22. Sankaram, M. B. and Marsh, D. (1993) Protein-lipid interactions with peripheral membrane proteins; in Protein-Lipid Interactions. Watts, A. (ed.), pp. 127-162, Elsevier, Amsterdam, The Netherlands
  23. Schlegel, A., Giddings, T. H., Ladinsky, Jr. M. S. and Kirkegaard, K. (1996) Cellular origin and ultrastructure of membranes induced during poliovirus infection, J. Virol. 70, 6576-6588
  24. Smith, W. L., Garavito, R. M. and Miller, S. F. (2001) Membrane protein structural bilology minireview series. J. Bio. Chem. 276, 32393-32394 https://doi.org/10.1074/jbc.R100044200
  25. Towner, J. S., Ho, T. V. and Semler, B. L. (1996) Determinants of membrane association for poliovirus protein 3AB. J. Bio. Chem. 271, 26810-26818 https://doi.org/10.1074/jbc.271.43.26810
  26. Tsien, R. Y. (1998) The green fluorescent protein. Annu Rev. Biochem. 67, 509-544 https://doi.org/10.1146/annurev.biochem.67.1.509
  27. Xia, Q., Jakana, J., Zhang, J. Q. and Zhou, Z. H. (2003) Structural comparisons of empty and full cytoplasmic polyhedrosis virus. J. Biol. Chem. 278, 1094-1100 https://doi.org/10.1074/jbc.M205964200
  28. Xie, D., Li, A., Wang, M. H., Fan, Z. W. and Fang, H. Q. (2005) LOCSVMPSI: a web server for subcellular localization of eukaryotic protein using SVM and profile of PSI-BLAST. Nucleic Acids Res. 33, 105-110
  29. Zhang, H., Zhang, J., Yu, X., Lu, X., Zhang, Q., Jakana, J. and Chen, D. H. (1999) Visualization of protein-RNA interactions in cytoplasmic polyhedrosis virus. J. Virol. 73, 1624-1629
  30. Zhao, S. L., Liang, C. Y., Hong, J. J., Xu, H. G. and Peng, H. Y. (2003a) Molecular characterization of segments 7-10 of Dendrolimus punctatus cytoplasmic polyhedrosis virus provides the complete genome. Virus Res. 94, 17-23 https://doi.org/10.1016/S0168-1702(03)00118-7
  31. Zhao, S. L., Liang, C. Y., Hong, J. J. and Peng, H. Y. (2003b) Genomic sequence analyses of segments 1 to 6 of Dendrolimus punctatus polyhedrosis virus. Arch. Virol. 148, 1357-1368
  32. Zhao, S. L., Liang, C. Y., Zhang, W. J., Tang, X. C. and Peng, H. Y. (2005c) Charaterization of the RNA-binding domain in the Dendrolimus punctatus cytoplasmic polyhedrosis virus nonstructural protein p44. Virus Res. 114, 80-88 https://doi.org/10.1016/j.virusres.2005.06.002
  33. Zhao, S. L., Liang, C. Y., Zhang, W. J., Tang, X. C. and Peng, H. Y. (2005d) Mapping the RNA-binding domain on the DpCPV VP44. Arch. Virol. 151, 273-283

Cited by

  1. Interactions among Dendrolimus punctatus cypovirus proteins and identification of the genomic segment encoding its A-spike vol.95, pp.Pt_7, 2014, https://doi.org/10.1099/vir.0.064022-0
  2. Molecular characterization of protein p50 of Dendrolimus punctatus cytoplasmic polyhedrosis virus vol.53, pp.1, 2013, https://doi.org/10.1002/jobm.201100488