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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Anti-CHH Antibody Causes Impaired Hyperglycemia in Penaeus monodon

  • Treerattrakool, Supattra (Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus) ;
  • Udomkit, Apinunt (Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus) ;
  • Panyim, Sakol (Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus)
  • Received : 2005.08.31
  • Accepted : 2006.03.20
  • Published : 2006.07.31
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Abstract

Crustacean hyperglycemic hormone (CHH) plays a major role in controlling glucose level in the haemolymph and also triggers important events during molting and reproductive cycles. In Penaeus monodon, three types of CHH, namely Pem-CHH1, Pem-CHH2 and Pem-CHH3, have been previously characterized. In this study, mouse polyclonal antibody was raised against recombinant Pem-CHH1 that was expressed in Escherichia coli. The anti-Pem-CHH1 antibody recognized all three types of Pem-CHHs but did not cross-react with either related hormone, molt-inhibiting hormone of P. monodon, or unrelated human growth hormone. The hyperglycemic activity in the extract from the eyestalk neural tissues was significantly depleted after incubating with anti-Pem-CHH antibody. Direct injection of the antibody into shrimp caused about 30-50% reduction in the haemolymph glucose level. The result demonstrates the ability of anti-Pem-CHH1 antibody to deplete the activity of CHH in vivo, and thus provides a possibility of using anti-Pem-CHH1 antibody to inhibit the hormone activity as a strategy to modulate growth and reproduction in this species.

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References

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