KSBB Journal

  • 제21권6호
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  • Pages.456-460
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  • 2006
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

전기자극 하에서 알콜 산화효소의 안정화연구; 당, 히드로젤, 계면활성제 효과

Stabilization of Alcohol Oxidase under Electrostimulation; Sugars, Hydrogels and Surfactants Effect

  • 김범수 (전북대학교 자연과학대학 분자생물학과) ;
  • 이강민 (전북대학교 자연과학대학 분자생물학과) ;
  • ;
  • 김경숙 (전북대학교 자연과학대학 분자생물학과)
  • Kim, Beom-Su (Department of Molecular Biology, College of Natural Science, Chonbuk University) ;
  • Lee, Kang-Min (Department of Molecular Biology, College of Natural Science, Chonbuk University) ;
  • Biellmann, J.F. (Institute of Chemistry, Louis Pasteur University) ;
  • Kim, Kyung-Suk (Department of Molecular Biology, College of Natural Science, Chonbuk University)
  • 발행 : 2006.12.30
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초록

효소는 환경과 조건에 따라 상이한 활성도를 나타내며, 전기 자극에 의하여 비활성화된 알콜 산화효소는 당, 계면활성제, 히드로젤 들에 의하여 안정화되었다. 이들 효소는 trehalose, TritonX-100, Brij에서 보다 안정하였으며, 이들 효소의 안정도는 Hansenula sp., P. pastoris, C. boidinii에서 분리된 효소가 순서적으로 보다 안정하였다. 전기자극하에서 효소의 안정화에 대한 이 연구는 생물공학, 단백질공학, 의료공학 분야에 널리 이용될 수 있으리라 사료된다.

We investigated the activity and stability of alcohol oxidase from Hansenula sp., Pichia pastoris, and Candida boidinii under the electric stimulation. The activity and stability of alcohol oxidase depended on electric output voltage, electric stimulation time. This inactivation of the enzyme under electric stimulation could be recovered by stabilizing additives such as sugars, surfactants and hydrogels. These alcohol oxidase was more stable in trehalose, Triton X-100, Brji solution and alcohol oxidase from Hansenula is more stable than that from P. pastoris, and C. boidinii. The stabilizing of enzymes against electric stimulation showed a great potential use of enzymes in biotechnology and medical engineering fields.

키워드

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