Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characterization of a Newly Isolated cis-1,2-Dichloroethylene and Aliphatic Compound-Degrading Bacterium, Clostridium sp. Strain KYT-1

  • Kim, Eun-Sook (United Graduate School of Agricultural Science, Gifu University) ;
  • Nomura, lzumi (Faculty of Applied Biological Sciences, Gifu University) ;
  • Hasegawa, Yuki (Faculty of Applied Biological Sciences, Gifu University) ;
  • Takamizawa, Kazuhiro (United Graduate School of Agricultural Science, Gifu University,Faculty of Applied Biological Sciences, Gifu University)
  • Published : 2006.12.31
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Abstract

A cis-1,2-dichloroethylene (cis-DCE)-degrading anaerobic bacterium, Clostridium sp. strain KYT-1, was isolated from a sediment sample collected from a landfill site in Nanji-do, Seoul, Korea. The KYT-1 strain is a gram-positive, endospore-forming, motile, rod-shaped anaerobic bacterium, of approximately $2.5{\sim}3.0\;{\mu}m$ in length. The degradation of cis-DCE is closely related with the growth of the KYT-1 strain, and it was stopped when the growth of the KYT-1 strain became constant. Although the pathway of cis-DCE degradation by strain KYT-1 remains to be further elucidated, no accumulation of the harmful intermediate, vinyl chloride (VC), was observed during anaerobic cis-DCE degradation. Strain KYT-1 proved able to degrade a variety of volatile organic compounds, including VC, isomers of DCE (1,1-dichloroethylene, trans-1,2-dichloroethylene, and cis-DCE), trichloroethylene, tetrachloroethylene, 1,2-dichloroethane, 1,1,1-trichloroethane, and 1,1,2-trichloroethane. Strain KYT-1 degraded cis-DCE at a range of temperatures from $15\;to\;37^{\circ}C$, with an optimum at $30^{\circ}C$, and at a pH range of 5.5 to 8.5, with an optimum at 7.0.

Keywords

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