Isolation of Off-flavors and Odors from Tuna Fish Oil Using Supercritical Carbon Dioxide

  • Roh, Hyung-Seob (Institute of Food Science, Faculty of Food Science and Biotechnology, pukyong National University) ;
  • Park, Ji-Yeon (Institute of Food Science, Faculty of Food Science and Biotechnology, pukyong National University) ;
  • Park, Sun-Young (School of Pharmacy and Medical Sciences, University of South Australia) ;
  • Chun, Byung-Soo (Institute of Food Science, Faculty of Food Science and Biotechnology, pukyong National University)
  • 발행 : 2006.12.31

초록

Off-flavors and unfavorable odors in tuna fish oil were successfully removed and identified using supercritical carbon dioxide extraction, while retaining variable compounds, polyunsaturated fatty acids such as EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid). Samples of oil were extracted in a 100 mL semi-batch stainless steel vessel under conditions which ranged from 8 to 20 MPa and $20\;to\;60^{\circ}C$ with solvent ($CO_{2}$) flows from 10 g/min. GC-MS was used to identify the main volatile components contributing to the off-flavors and odors which included 2-methyl-1-propanol, 2,4-hexadienal, cyclopropane, and octadiene. Analyses of oil extracted at $40^{\circ}C$, 20 MPa showed a 99.8% reduction in dimethyl disulfide. Other significant off-flavors identified were 2-methyl-butene, 3-hydroxy butanal and ethylbenzene.

키워드

참고문헌

  1. Klinkesorn, U., A. H-Kittikun, P. Chinachoti, and P. Sophanodora (2004) Chemical transesterification of tuna oil to enriched omega-3 polyunsaturated fatty acids. Food Chem. 87: 415-421 https://doi.org/10.1016/j.foodchem.2003.12.021
  2. Lands, W. E. M. (1986) Fish and Human Health. Academic Press, Orlando, FL, USA
  3. Kang, K.-Y., D.-H. Ahn, S.-M. Jung, D.-H. Kim, and B.- S. Chun (2005) Separation of protein and fatty acids from tuna viscera using supercritical carbon dioxide. Biotechnol. Bioprocess Eng. 10: 315-321 https://doi.org/10.1007/BF02931848
  4. Rambjor, G. S., A. I. Walen, S. L. Winsor, and W. S. Harris (1996) Eicosapentaenoic acid is primarily resposible for hypotriglyceridemic effect of fish oil in humans. Lipids 31: 45-49 https://doi.org/10.1007/BF02637050
  5. Kamali, R. A., J. Marsh, and C. Fuchs (1984) Effect of n- 3 fatty acid on growth of rat mammary tumor. J. Natl. Cancer Inst. 75: 457-462
  6. Stahl, E., K. W. Quirin, and D. Gerard (1988) Applications of Dense Gases to Extraction and Refining, in Dense Gases for Extraction and Refining. Springer-Verlag, Berlin, Germany
  7. Riha, V. and G. Brunner (2000) Separation of fish oil ethyl esters with supercritical carbon dioxide. J. Supercrit. Fluids 17: 55-64 https://doi.org/10.1016/S0896-8446(99)00038-8
  8. McHugh, M. A. and V. J. Krukonis (1986) Processing Pharmaceuticals, Natural Products, and Specialty Chemicals, and Waste Streams. Supercritical Fluids Extraction: Principles and Practice (Butterworth-Heinemann Series in Chemical Engineering). Butterworth-Heinemann, Sthoneham, MA, Quebec, Canada
  9. Kim, B. S., Y. K. Hong, and W. H. Hong (2004) Effect of salts on the extraction characteristics of succinic acid by predispersed solvent extraction. Biotechnol. Bioprocess Eng. 9: 207-211 https://doi.org/10.1007/BF02942294
  10. Esquivel, M. M., N. M. Bandarra, I. Fontan, and M. G. Bernardo-Gil (1997) Supercritical carbon dioxide extraction of sardine Sardina pilchardus oil. Lebensm. Wiss. Technol. 30: 715-720 https://doi.org/10.1006/fstl.1997.0249
  11. Chun, B.-S., Y.-W. Kim, S.-K. Song, S.-K. Kim, and G. T. Wilkinson (1998) Extraction and fractionation of lipids from squid internal organs using supercritical carbon dioxide with entrainer. Proceedings of 5th ISASF Meeting. March 23-25. Nice, France
  12. Chun, B.-S., K.-Y. Kang, D.-H. Ahn, and G. T. Wilkilnson (2005) Extraction of liquids and cholesterol from squid oil with supercritical carbon dioxide. Kor. J. Chem. Eng. 22: 399-405 https://doi.org/10.1007/BF02719418
  13. Timon, M. L., J. Ventanas, L. Martin, J. F. Tejeda, and C. Garcia (1998) Volatile compounds in supercritical carbon dioxide extracts of Iberian ham. J. Agric. Food Chem. 46: 5143-5150 https://doi.org/10.1021/jf980652v
  14. Taylor, D. L. and D. K. Larick (1995) Investigations into the effect of supercritical carbon dioxide extraction on the fatty acid and volatile profiles of cooked chicken. J. Agric. Food Chem. 43: 2369-2374 https://doi.org/10.1021/jf00057a010
  15. Kim, H.-S., S.-Y. Lee, B.-Y. Kim, E.-K. Lee, J.-H. Ryu, and G.-B. Lim (2004) Effects of modifiers on the supercritical $CO_2$ extraction of glycyrrhizin from licorice and the morphology of licorice tissue after extraction. Biotechnol. Bioprocess Eng. 9: 447-453 https://doi.org/10.1007/BF02933484
  16. Peralta, R. R., M. Shimoda, and Y. Osajima (1996) Further identification of volatile compounds in fish sauce. J. Agric. Food Chem. 44: 3606-3610 https://doi.org/10.1021/jf9603036
  17. US EPA Method To-17A (1999) Compendium of Methods for the Determination of Toxic Organic Compounds in Ambient Air, Determination of Volatile Organic Compounds in Ambient Air Using Active Sampling Onto Sorbent Tube. 2nd ed., pp. 9-22. Center for Environmental Research Information, Ohio, USA
  18. Hardardottir, I. and J. E. Kinsella (1988) Extraction of lipid and cholesterol from fish muscle with supercritical fluids. J. Food Sci. 3: 1656-1661