Enhancement of Carbon Dioxide Fixation by Alteration of Illumination during Chlorella Vulgaris-Buitenzorg's Growth

  • 발행 : 2006.12.31

초록

Alteration of illumination with optimum carbon dioxide fixation-based curve in this research successfully enhanced the $CO_{2}-fixation\;(q_CO_{2}$ capability of Chlorella vulgaris Buitenzorg cultivated in a bubble column photo bioreactor. The level of $CO_{2}$ fixation was up to 1.91 times that observed from cultivation with intensification of illumination on an optimum growth-based curve. During 144 h of cultivation, alteration of light intensity on an optimum $CO_{2}-fixation-based$ curve produced a $q_CO_{2}$ of $12.8\;h^{-1}$. Meanwhile, alteration of light intensity with a growth-based curve only produced a $q_CO_{2}$ of $6.68\;h^{-1}$. Increases in light intensity based on a curve of optimum $CO_{2}-fixation$ produced a final cell concentration of about 5.78 g/L. Both cultivation methods were carried out under ambient pressure at a temperature of $29^{\circ}C$ with a superficial gas velocity of $2.4\;m/h(U_{G}$. Cells were grown on Beneck medium in a 1.0 L Bubble Column Photo bioreactor illuminated by a Phillips Halogen Lamp (20 W/12 V/50 Hz). The inlet gas had a carbon dioxide content of 10%.

키워드

참고문헌

  1. Stewart, C. and M. A. Hessami (2004) Propositions of sustainable methods of carbon dioxide separation and disposal. Proc. Int'l Conf. Sustainability Eng. Sci., Auckland, New Zealand 55.1-55.8
  2. Kaplan, A., M. R. Badger, and J. A. Berry (1980) Photosynthesis and the intracellular inorganic carbon pool in the bluegreen alga Anabaena variabilis: Response to external $CO_2$ concentration. Planta 149: 219-226 https://doi.org/10.1007/BF00384557
  3. Ohtaguchi, K. (2000) Soft energy path synthesis from carbon dioxide to biofuel ethanol through cyanobacterial biotechnology. Technology 7S: 175-188
  4. Ohtaguchi, K. and A. Wijanarko (2002) Elevation of the efficiency of cyanobacterial carbon dioxide removal by monoethanolamine solution. Technology 8: 267-286
  5. Wijanarko, A., K. Asami, and K. Ohtaguchi (2004) The kinetics of growth and the $CO_2$ concentrating mechanism of the filamentous cyanobacterium Anabaena cylindrica in a bubble column. J. Chem. Eng. Jpn. 37: 1019-1025 https://doi.org/10.1252/jcej.37.1019
  6. Wijanarko, A. and K. Ohtaguchi (2004) Carbon dioxide removal and biomass production by Anabaena cylindrica IAM M1 using reactor in series. Stud. Surf. Sci. Catal. 153: 461-468 https://doi.org/10.1016/S0167-2991(04)80296-9
  7. Wirosaputro, S. (2002) Chlorella untuk Kesehatan Global, Teknik Budidaya dan Pengolahan. Gajahmada University Press, Yogyakarta, Indonesia
  8. Ogbonna, J. C., H. Yada, and H. Tanaka (1995) Kinetic study on light-limited batch cultivation of photosynthetic cells. J. Ferment. Bioeng. 80: 259-264 https://doi.org/10.1016/0922-338X(95)90826-L
  9. Falkowski, P. G. and T. G. Owens (1980) Light-shade adaptation. Plant Physiol. 66: 592-595 https://doi.org/10.1104/pp.66.4.592
  10. Oquist, G., J. M. Anderson, S. McCaffery, and W. S. Chow (1992) Mechanistic differences in photoinhibition of sun and shade plants. Planta 188: 422-431
  11. Wijanarko, A. and K. Ohtaguchi (2003) Alteration of light illumination during microbial growth, an enhancement effort of biomass production and $CO_2$-fixation of psychrophilic cyanobacterium Anabaena cylindrica IAM M1. Comp. Biochem. Physiol. 134: S214
  12. Hirata, S., M. Taya, and S. Tone (1996) Characterization of Chlorella cell cultures in batch and continuous operations under a photoautotrophic condition. J. Chem. Eng. Jpn. 29: 953-959 https://doi.org/10.1252/jcej.29.953
  13. Hirata, S., M. Taya, and S. Tone (1998) Continuous cultures of Spirulina platensis under photoautotrophic conditions with change in light intensity. J. Chem. Eng. Jpn. 31: 636-639 https://doi.org/10.1252/jcej.31.636