International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Isolation and Culture of Entomopathogenic Fungus, Cordyceps sphecocephala

  • Nam, Sung-Hee (National Institute of Agricultural Science and Technology, Department of Agricultural Biology, R.D.A.) ;
  • Li, Chun-Ru (Anhui Agricultural University) ;
  • Hong, In-Pyo (National Institute of Agricultural Science and Technology, Department of Agricultural Biology, R.D.A.) ;
  • Sung, Kyu-Byoung (National Institute of Agricultural Science and Technology, Department of Agricultural Biology, R.D.A.) ;
  • Kang, Seok-Woo (National Institute of Agricultural Science and Technology, Department of Agricultural Biology, R.D.A.) ;
  • Fan, Mei-Zhen (Anhui Agricultural University) ;
  • Li, Zeng-Zhi (Anhui Agricultural University)
  • Published : 2006.12.31
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Abstract

In this study, morphology of perithecia, asci, ascospores, etc. of C. sphecocephala were examined for its telemorphic characteristics. Its colony grew up to 32 mm in diameter on potato dextrose agar (PDA) for 30 days under the condition of $24{\pm}1^{\circ}C$. PDBLA and PDBAA media were selected as optimal media for C. sphecocephala, on which the growth was 1.5 times as fast as on PDA medium. Moreover, PDBLA medium induced successfully the synnemata of anamorphic state. C. sphecocephala was able to be proliferated in vitro on both larva and adult of honeybee drone as its substrate. After inoculated onto the drone larva, it produced mycelium at $24{\pm}1^{\circ}C$, with the maximum yield up to $67{\pm}3mg$ on the $50^{th}$ day.

Keywords

References

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