Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Lipids from the Roots of Canna generalis

칸나(Canna generalis)의 뿌리로부터 지질화합물의 분리.동정

  • Bang, Myun-Ho (Ganghwa Agricultural R&D Center) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Yang, Hye-Jung (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Han, Min-Woo (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Youn-Hyung (Major in Horticultural Biotechnology, Kyung Hee University)
  • 방면호 (강화군 특화작목연구소) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 양혜정 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한민우 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이윤형 (경희대학교 생명과학대학)
  • Published : 2006.12.31
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Abstract

Canna generalis was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. from the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as $\beta$-sitosterol(1), linoleic acid methyl ester(2),1-O-oleoyl-2-O-linoleoyl-3-O-$\beta$-D-galactopyranosyl-sn-glycerol(3), and daucosterol(4). They were the first to be isolated from Canna generalis.

칸나의 뿌리를 80% MeOH용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획과 n-BuOH 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography로 정제하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조는 NHR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, $\beta$-sitosterol(1), linoleic aicd methyl ester(2), monoglycosyldiglyceride(3), daucosterol(4)으로 동정하였다. 이 화합물들은 칸나에서 처음으로 분리되었다.

Keywords

References

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