Fluorometric Analysis of Transient Gene Expression in Broccoli Seedlings

  • Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu) ;
  • Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
  • Published : 2006.12.31

Abstract

Keywords

References

  1. Henzi, M. X., Christey, M. C. and Mcneil, D. L. (2000) Factors that influence Agrobacterium rhizogenes-mediated transformation of broccoli (Brassica oleracea L. var. italica) Plant Cell Rep. 19, 994-999 https://doi.org/10.1007/s002990000221
  2. Malyshenko, S. I., Tyulkina, L. G., Zvereva, S. D. and Raldugina, G. N. (2003) Transgenic Brassica campestris plants expressing the gfp gene. Russ. J. Plant. Physiol. 50, 276-281 https://doi.org/10.1023/A:1022997803459
  3. Metz, T. D., Roush, R. T., Tang, J. D., Shelton, A. M. and Earle, E. D. (1995) Transgenic broccoli expressing Bacillus thuringiensis insecticidal crystal protein: implications for pest resistance management strategies. Mol. Breed. 1, 309- 317 https://doi.org/10.1007/BF01248408
  4. Downs, C. G., Christey, M. C., Davies, K. M., Seelye, J. F., Sinclair, B. K. and Stevenson, D. G. (1994) Hairy roots of Brassica napus: 2. Glutamine synthase overexpression alters ammonia assimilation and the response to phosphinitricin. Plant Cell 14, 41-46
  5. Andrews, L. B. and Curtis, W. R. (2005) Comparison of transient protein expression in tobacco leaves and plant suspension culture. Biotechnol. Prog. 21, 946-952 https://doi.org/10.1021/bp049569k
  6. Fisher, R., Vaquero-Martin, C., Sack, M., Drossard, J., Emans, N. and Commandeur, U. (1999) Towards molecular farming in the future: transient protein expression in plants. Biotechnol. Appl. Biochem. 30, 99-100
  7. Vaquero, C., Sack, M., Chandler, J., Drossard, J., Schuster, F., Monecke, M., Schillberg, S. and Fisher, R. (1999) Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc. Natl. Acad. Sci. USA 96, 11128-11133
  8. Jefferson, R. A. (1987) Assaying chimeric genes in plants: the gus gene fusion system. Plant Mol. Biol. Rep. 5, 387- 405 https://doi.org/10.1007/BF02667740
  9. Flores Solic, J. I., Mlejnek, P., Studena, K. and Prochazka, S. (2003) Application of sonication-assisted Agrobacteriummediated transformation in Chenopodium rubrum L. Plant Soil Environ. 49, 255-260 https://doi.org/10.1080/00380768.2003.10410005
  10. Singh, N. and Chawla, H. S. (1999) Use of silicon carbide fibers for Agrobacterium-mediated transformation in wheat. Curr. Sci. 76, 1483-1485
  11. Hoshi, Y., Kondo, M., Mori, S., Adachi, Y., Nakano, M. and Kobayasi, H. (2004) Production of transgenic lily plant by Agrobacterium-mediated transformation. Plant Cell Rep. 22, 359-364 https://doi.org/10.1007/s00299-003-0700-z