Suppression of Clubroot Formation in Chinese Cabbage by the Chitin Compost and Broth

  • Jin Rong De (Division of Applied BioScience and Biotechnology, and Environmental-Friendly Agriculture Research Center, Chonnam National University) ;
  • Han Tae-o (Institute of Agricultural Science and Technology, Chonnam National University, Gwangju) ;
  • Kim Yong-oong (Division of Applied BioScience and Biotechnology, and Environmental-Friendly Agriculture Research Center, Chonnam National University) ;
  • Kim Kil-ong (Division of Applied BioScience and Biotechnology, and Environmental-Friendly Agriculture Research Center, Chonnam National University)
  • Published : 2006.12.31

Abstract

Chitin compost and broth were used to suppress club root. Individual cabbage seedlings were transplanted into pots(3500 ml) containing a mixture of 3% chitin compost and 50 ml of chitin broth (T1) or the same quantity control compost and control compost broth(T2). The media in each pot was then infected with Plasmodiophora brassicae. Samples were taken at 6, 7 and 8 weeks after transplanting. The population of chitinase producing bacteria in T1 was consistently larger than that observed in T2. Chitinase activity in the T1 rhizosphere was two-fold greater than that of T2 at each time point observed. Shoot dry weight, leaf number and leaf area in T1 were enhanced 20%, 10% and 12% relative to those seen in T2, respectively. The disease index and root mortality at 8 weeks after transplanting were reduced by 50% and 25% in T1 compared to T2, respectively. Results presented in this study are strongly indicative that chitin compost and broth suppress clubroot in Chinese cabbage.

Keywords

References

  1. Kim, C. H., Cho, W. D. and Kim, H. M. (2000) Some environment factors affecting decay of root galls in club root disease of Chinese cabbage. Korean J. of Pest. Sci. 4, 61-65
  2. Kim, C. H., Cho, W. D. and Kim, H. M. (2000) Distribution of Plasmodiophora brassicae causing clubroot disease of Chinese cabbage in soil. Plant Dis. Res. 6, 27-33
  3. Gao, Y. J., Shin, H. D., An, K. K., Lee, S. K., Lee, J. K., Cha, B. J. and Cha, J. S. (1998) Plant diseases by the plasmodiophoromycetes. In: Plant pathology, pp. 267-27, World Science Publishers, Seoul, Korea
  4. Narisawa, K., Ohki, K. T. and Hashiba, T. (2000) Suppression of clubroot and Verticillium yellows in Chinese cabbage in the field by the root endophytic fungus, Heteroconium chaetospira. Plant Pathol. 49, 141-146 https://doi.org/10.1046/j.1365-3059.2000.00425.x
  5. Narisawa, K., Tokumasu, S. and Hashiba, T. (1998) Suppression of clubroot formation in Chinese cabbage by the root endophytic fungus, Heteroconium chaetospira. Plant Pathol. 47, 206-210 https://doi.org/10.1046/j.1365-3059.1998.00225.x
  6. Murakami, H., Tsushima, S. and Shishido, Y. (2000). Soil suppressiveness to clubroot disease of Chinese cabbage caused by Plasmodiophora brassicae. Soil Biol. Biochem. 32, 1637-1642 https://doi.org/10.1016/S0038-0717(00)00079-1
  7. Murakami, H., Tsushima, S., Akimoto T., Murakami, K., Goto, I. and Shishido, Y. (2000) Effect of growing leafy daikon (Raphanus sativus) on populations of Plasmodiophora brassicae (clubroot). Plant Pathol. 49, 584-589 https://doi.org/10.1046/j.1365-3059.2000.00495.x
  8. Jung, W. J., An, K. N., Jin, Y. L., Park, R. D., Lim, K. T., Kim, K. Y. and Kim, T. H. (2003) Biological control of damping-off caused by Rhizoctonia solani using chitinaseproducing Paenibacillus illinoisensis KJA-424. Soil Biol. Biochem. 35, 1261-1264 https://doi.org/10.1016/S0038-0717(03)00187-1
  9. Chang, W. T., Chen C. S. and Wang, S. L. (2003) An antifungal produced by Bacillus cereus with shrimp and crab shell powder as carbon source. Cur. Microbiol. 47, 102-108 https://doi.org/10.1007/s00284-002-3955-7
  10. Kim, K. J. (1998) Chapter I: Introduction. In Gen. Mycol., World Science Publishers, Seoul, Korea
  11. Lee, H. J, Park. K. H., Shim J. H., Park, R. D., Kim Y. W., Cho, J. Y., Kim, Y. C., Krishnan H. B. and Kim, K. Y. (2005) Isolation and identification of low molecular weight compounds produced by Bacillus subtilis HJ927 and their biocontrol effect on the late blight of pepper (Capsicum annuum L.). Korean J. Soil Sci. Fertil. 38, 25-31
  12. Mian, I. H., Godoy, G., Shelby, R. A., Rodríguez-Kábana, R. and Morgan-Jones, G. (1982) Chitin amendment for control of Meloidogyne arenaria in infested soil. Nematolopica 12, 71-84
  13. Wang, S. L., Shih I. L., Wang C. H., Tseng K. C., Chang, W. T., Twu, Y. K., Ro, J. J. and Wang C. L. (2002) Production of antifungal compounds from chitin by Bacillus subtilis. Enz. Microbial Technol. 31, 321-328 https://doi.org/10.1016/S0141-0229(02)00130-8
  14. Trotta, A., Varese, G. C., Gnavi, E., Fusconi, A., Sampo, S. and Berta, G. (1996) Interaction between the soilborne root pathogen Phytophthora nicotianae var. parasitica and the arbuscular mycorrhizal fungus Glomos mosseae in tomato plants. Plant Soil 185, 199-209 https://doi.org/10.1007/BF02257525
  15. Yedidia, I., Benhamou, N., Kapulnik, Y. and Chet, I. (2000) Induction and accumulation of PR protein activities during early stages of root colonization by the mycoparasite Trichoderma harzianum strain T-203. Plant Physiol. Biochem. 38, 863-873 https://doi.org/10.1016/S0981-9428(00)01198-0
  16. Wollum, A. G. (1982) Cultural methods for soil microorganism. In Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties, Page, A. L., Miler, R. H. and Keeney, D. R. (eds), pp. 781-02, American Society of Agronomy Madison, Wisconsin, USA
  17. Liu, X. Z. and Huang, B. R. (2000) Carbohydrate accumulation in relation to heat stress tolerance in two creeping bentgrass cultivars. J. Am. Soci. Hort. Sci. 125, 442-447
  18. Yang, C. S. and Kim, J. S. (2002) Plasmodiophora brassicae. In Laboratory methods for soil microorganisms, pp. 107-121, World Science Publishers, Seoul, Korea
  19. Chae, D. H. (2003) Control of late blight (Phytophthora capsici) in pepper plant with a compost containing multitude of chitinase producing bacteria. Master's Thesis, Chonnam National University, Gwangju, Republic of Korea
  20. Carolina, F., Rodrigo, R., Prem, W. and Joseph, W. K. (2001) Induced soil suppressiveness to a root-knot nematode species by a nematicides. Biol. Control 22, 103-114 https://doi.org/10.1006/bcon.2001.0961
  21. Melent'ev, A. I., Aktuganov, G. E. and Galimzyanova, N. F. (2001) The Role of Chitinase in the Antifungal Activity of Bacillus sp.739. Microbiology 70, 636-641
  22. Jin R. D., Suh, J. W., Park, R. D., Kim, Y. W., Krishnan H. B. and Kim, K. Y. (2005) Effect of chitin compost and broth on biological control of Meloidogyne incognita in tomato (Lycopersicon esculentum Mill). Nematology 7, 125-132 https://doi.org/10.1163/1568541054192171