Mycobiology

The Korean Society of Mycology (한국균학회)
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Effect of Preservation Periods and Subcultures on Fruiting Body Formation of Cordyceps militaris In Vitro

  • Sung, Jae-Mo (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University) ;
  • Park, Young-Jin (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University) ;
  • Lee, Je-O (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University) ;
  • Han, Sang-Kuk (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University) ;
  • Lee, Won-Ho (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University) ;
  • Choi, Sung-Keun (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University) ;
  • Shrestha, Bhushan (Department of Applied Biology and Entomopathogenic Fungal Culture Collection (EFCC), Kangwon National University)
  • Published : 2006.12.31
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Abstract

Effects of various preservation periods and subcultures on fruiting body formation of Cordyceps militaris were investigated using EFCC C-10995 single ascospore strains. Fruiting body formation by original strains was profuse when preserved at $4^{\circ}C$ for $5{\sim}6$ months. Fruiting from subcultures was stable till second to sixth subcultures, after which it decreased sharply. The more the colony color of subcultures changed, the less the fruiting bodies formed. Liquid inoculum preparation of single ascospore strains in the same or separate broths did not affect fruiting body formation. Similarly, two strains C-10995-3 and C-10995-6 in different numbers during liquid inoculum preparation produced similar fruiting bodies.

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References

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