Biotechnology and Bioprocess Engineering:BBE

  • 제11권4호
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  • Pages.364-367
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  • 2006
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Effects of Silkworm Hemolymph and Cartilage-specific Extracellular Matrices on Chondrocytes and Periosteum-derived Progenitor Cells

  • 발행 : 2006.08.30
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초록

In order to enhance the repair of defects in articular cartilage via cell therapy with autologous chondrocytes, as well as with periosteum-derived progenitor cells (PDPCs), silkworm hemolymph (SH) and a variety of cartilage-specific extracellular matrices (ECMs) including type II collagen, proline, chondroitin 4-sulfate, and chondroitin 6-sulfate were assessed with regard to their efficacy as media supplements. SH, a known anti-apoptotic agent, was found to enhance cell growth, as was shown by the results of a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to the results of reverse transcriptase polymerase chain reaction (RT-PCR) analyses, the cartilage-specific ECMs were found to stimulate the expression of hyaline cartilage-specific genes, most notably type II collagen and Sox9, in monolayer cultures of PDPCs.

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참고문헌

  1. Hunziker, E. B. (2000) Articular cartilage repair: Problems and perspectives. Biorheology 37: 163-164
  2. Evans, C. H. and H. I. Georgescu (1983) Observations on the senescence of cells derived from articular cartilage. Mech. Ageing Dev. 22: 179-191 https://doi.org/10.1016/0047-6374(83)90111-2
  3. Malpeli, M., N. Randazzo, R. Cancedda, and B. Dozin (2004) Serum-free growth medium sustains commitment of human articular chondrocyte through maintenance of Sox9 expression. Tissue Eng. 10: 145-155 https://doi.org/10.1089/107632704322791790
  4. Stewart, M. C., K. M. Saunders, N. Burton-Wurster, and J. N. Macleod (2000) Phenotypic stability of articular chondrocytes in vitro: The effects of culture models, bone morphogenetic protein 2, and serum supplementation. J. Bone Miner. Res. 15: 166-174 https://doi.org/10.1359/jbmr.2000.15.1.166
  5. Thomas, B., S. Thirion, L. Humbert, L. Tan, M. B. Goldring, G. Bereziat, and F. Berenbaum (2002) Differentiation regulates interleukin-1beta-induced cyclooxygenase- 2 in human articular chondrocytes: role of p38 mitogen-activated protein kinase. Biochem. J. 362: 367-373 https://doi.org/10.1042/0264-6021:3620367
  6. Thirion, S. and F. Berenbaum (2004) Culture and phenotyping of chondrocytes in primary culture. Methods Mol. Med. 100: 1-14
  7. Short, B., N. Brouard, T. Occhiodoro-Scott, A. Ramakrishnan, and P. J. Simmons (2003) Mesenchymal stem cells. Arch. Med. Res. 34: 565-571 https://doi.org/10.1016/j.arcmed.2003.09.007
  8. Barry, F., R. E. Boynton, B. Liu, and J. M. Murphy (2001) Chondrogenic differentiation of mesenchymal stem cells from bone marrow: Differentiation-dependent gene expression of matrix components. Exp. Cell Res. 268: 189-200 https://doi.org/10.1006/excr.2001.5278
  9. Lim, S. M., Y. S. Choi, H. C. Shin, C. W. Lee, and D.-I. Kim (2005) Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis. Biotechnol. Lett. 27: 607-611 https://doi.org/10.1007/s10529-005-3625-5
  10. Freed, L. E., R. Langer, I. Martin, N. R. Pellis, and G. Vunjak-Novakovic (1997) Tissue engineering of cartilage in space. Proc. Natl. Acad. Sci. USA 94: 13885-13890
  11. Freed, L. E., A. P. Hollander, I. Martin, J. R. Barry, R. Langer, and G. Vunjak-Novakovic (1998) Chondrogenesis in a cell-polymer-bioreactor system. Exp. Cell Res. 240: 58-65 https://doi.org/10.1006/excr.1998.4010
  12. Chen, H. C., H. P. Lee, M. L. Sung, C. J. Liao, and Y. C. Hu (2004) A novel rotating-shaft bioreactor for two-phase cultivation of tissue-engineered cartilage. Biotechnol. Prog. 20: 1802-1809 https://doi.org/10.1021/bp049740s
  13. Yaeger, P. C., T. L. Masi, J. L. de Ortiz, F. Binette, R. Tubo, and J. M. McPherson (1997) Synergistic action of transforming growth factor-beta and insulin-like growth factor- I induces expression of type II collagen and aggrecan genes in adult human articular chondrocytes. Exp. Cell Res. 237: 318-325 https://doi.org/10.1006/excr.1997.3781
  14. Bassleer, C., L. Rovati, and P. Franchimont (1998) Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro. Osteoarthritis Cartilage 6: 427-434 https://doi.org/10.1053/joca.1998.0146
  15. Kipnes, J., A. L. Carlberg, G. A. Loredo, J. Lawler, R. S. Tuan, and D. J. Hall (2003) Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Osteoarthritis Cartilage 11: 442-454 https://doi.org/10.1016/S1063-4584(03)00055-4
  16. Dodge, G. R. and S. A. Jimenez (2003) Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase- 3 synthesized by cultured human osteoarthritis articular chondrocytes. Osteoarthritis Cartilage 11: 424-432 https://doi.org/10.1016/S1063-4584(03)00052-9
  17. Philp, D., S. S. Chen, W. Fitzgerald, J. Orenstein, L. Margolis, and H. K. Kleinman (2005) Complex extracellular matrices promote tissue-specific stem cell differentiation. Stem Cells 23: 288-296 https://doi.org/10.1634/stemcells.2002-0109
  18. Kim, E. J., W. J. Rhee, and T. H. Park (2001) Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori. Biochem. Biophys. Res. Commun. 285: 224-228 https://doi.org/10.1006/bbrc.2001.5148
  19. Kim, J. E., E. J. Kim, W. J. Rhee, and T. H. Park (2005) Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph. Biotechnol. Bioprocess Eng. 10: 353-356 https://doi.org/10.1007/BF02931854
  20. Choi, S. S., W. J. Rhee, and T. H. Park (2002) Inhibition of human cell apoptosis by silkworm hemolymph. Biotechnol. Prog. 18: 874-878 https://doi.org/10.1021/bp020001q
  21. Oh, I. S. and H. G. Kim (2004) Vascular endothelial growth factor upregulates follistatin in human umbilical vein endothelial cells. Biotechnol. Bioprocess Eng. 9: 201-206 https://doi.org/10.1007/BF02942293
  22. Girotto, D., S. Urbani, P. Brun, D. Renier, R. Barbucci, and G. Abatangelo (2003) Tissue-specific gene expression in chondrocytes grown on three-dimensional hyaluronic acid scaffolds. Biomaterials 24: 3265-3275 https://doi.org/10.1016/S0142-9612(03)00160-1