Biotechnology and Bioprocess Engineering:BBE

  • 제11권2호
  • /
  • Pages.164-167
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  • 2006
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Production of Hepatitis B Core Antigen in a Stirred Tank Bioreactor: The Influence of Temperature and Agitation

  • Tey, Beng Ti (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Chua, Mung Ing (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Chua, Ghee Sung (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Ng, Michelle Yeen Tan (Institute of Bioscience, University Putra Malaysia) ;
  • Biak, Dayang Radiah Awang (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Tan, Wen Siang (Institute of Bioscience, University Putra Malaysia) ;
  • Ling, Tau Chuan (Institute of Bioscience, University Putra Malaysia)
  • 발행 : 2006.04.30
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초록

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli$(0.844 h^{-1})$ was achieved at a temperature of $37^{\circ}C$ and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W3110lQ in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of $44^{\circ}C$ and an agitation speed of 250 rpm. The relative protein concentration at $44^{\circ}C$ is 30 and 6% higher compared to that at 30 and $37^{\circ}C$, respectively.

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