Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhanced Clavulanic Acid Production in Streptomyces clavuligerus NRRL3585 by Overexpression of Regulatory Genes

  • Hung, Trinh Viet (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University) ;
  • Ishida, Kenji (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University) ;
  • Parajuli, Niranjan (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University) ;
  • Liou, Kwang-Kyoung (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University) ;
  • Lee, Hei-Chan (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University) ;
  • Sohng, Jae-Kyung (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, Sun Moon University)
  • Published : 2006.04.30
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Abstract

We constructed four recombinant plasm ids to enhance the production of clavulanic acid (CA) in Streptomyces clavuligerus NRRL3585: (1) pIBRHL1, which includes ccaR, a pathway-specific regulatory gene involved in cephamycin C and CA biosynthesis; (2) pIBRHL2, containing claR, again a regulatory gene, which controls the late steps of CA biosynthesis; (3) pGIBR containing afsR-p, a global regulatory gene from Streptomyces peucetius; and (4) pKS, which harbors all of the genes (ccaR/ claR/ afsR-p). The plasmids were expressed in S. clavuligerus NRRL3585 along with the $ermE^*$ promoter. All of them enhanced the production of CA; 2.5-fold overproduction for pIBRHL1, 1.5-fold for pIBRHL2, 1.6-fold for pGIBR, and 1.5-fold for pKS compared to the wild type.

Keywords

References

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