Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Morphology and Rheology on Neo-fructosyltransferase Production by Penicillium citrinum

  • Lim, Jung-Soo (Department of Chemical and Biological Engineering, Korea University) ;
  • Lee, Jong-Ho (Department of Chemical and Biological Engineering, Korea University) ;
  • Kim, Jung-Mo (Department of Chemical and Biological Engineering, Korea University) ;
  • Park, Seung-Won (Food Ingredient Division, CJ Foods R&D, CJ Corp.) ;
  • Kim, Seung-Wook (Department of Chemical and Biological Engineering, Korea University)
  • Published : 2006.04.30
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Abstract

In this study, we investigated the relationship between the morphology and the rheological properties of Penicillium citrinum to improve the production of neo-fructosyltransferase (neo-FTase). In a 2.5 L bioreactor culture of P. citrinum, it was observed that agitation speed and aeration rate had significant effects on the production of neo-FTase and that maximum cell mass and neo-FTase production obtained at 500 rpm and 1.5vvm were 8.14 g/L and $53.2{\times}10^{-3} U/mL$, respectively. Cell mass and neo-FTase production increased to 91.53 and 25.17%, respectively. In the morphology and rheology studies, P. citrinum showed a typical pellet morphology that was explained by a shaving mechanism; this phenomenon was significantly affected by carbon sources. The rheology of neo-FTase fermentation by P. citrinum was dependent on cell growth and fungal morphology.

Keywords

References

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