Molecular Screening and Characterization of Antiviral Potatoes

  • Tripathi, Giriraj (Department of Biotechnology, College of Natural Sciences, Chosun University) ;
  • Li, Hongxain (Department of Biotechnology, College of Natural Sciences, Chosun University) ;
  • Park, Jae-Kyun (Department of Biotechnology, College of Natural Sciences, Chosun University) ;
  • Park, Yoon-Kyung (Department of Biomaterials, Graduated School, Chosun Unviersity) ;
  • Cheong, Hyeon-Sook (Department of Biotechnology, College of Natural Sciences, Chosun University)
  • Published : 2006.04.30

Abstract

Potato plants carrying the Ry gene are extremely resistance to a number of potyviruses, but it is not known which variety expressed the resistance. In this investigation, combined classical and molecular techniques were used to identify virus resistance potatoes. Mechanical inoculation of 32 varieties of Korean potato cultivars, with potato virus Y (PVY), induced various symptoms, such as mosaic, yellowing, necrosis, mottle, vein clearing and vein bending. Different virus spreading patterns were observed, such as highly sensitive, moderate and resistant to $PVY^o$ inoculated leaves in different cultivars. From the results of double antibody sandwich-enzyme links immunosorbant assays (DAS-ELISA), coupled with reverse transcription polymerase chain reaction (RT-PCR), Winter valley and Golden valley were found to be highly susceptible and resistant cultivars to $PVY^o$ respectively. TEM was used as a complementary method to conform the localization of the virus in leaf tissues. TEM detect virus particles in Golden valley, where, ELISA and RT-PCR were unable to detect the CP gene. However, the interior part of the tissues was severely deformed in $PVY^o$ infected Winter valley, than Golden valley The Ry gene is involved in an induced response in $PVY^o$ infected Golden valley plants. The methods described in this study could be applied for the screening and development of antiviral potatoes.

Keywords

References

  1. Valkonen, J. P. T. (1994) Natural genes and mechanisms for resistance to viruses in cultivated and wild potato species (Solanum spp.). Plant Breed. 112: 1-16 https://doi.org/10.1111/j.1439-0523.1994.tb01270.x
  2. Hinrichs, J., S. Berger, and J. G. Shaw (1998) A hypersensitive response-like mechanism is involved in resistance of potato plants bearing the Ry(sto) gene to the potyviruses potato virus Y and tobacco etch virus. J. Gen. Virol. 79: 167-176 https://doi.org/10.1099/0022-1317-79-1-167
  3. Barker, H. (1993) Incidence of potato viras Y infection in seed and ware tubers of the potato cv. Record. Ann. Appl. Biol. 124: 179-183 https://doi.org/10.1111/j.1744-7348.1994.tb04126.x
  4. Jones, D. A. C., J. A. T. Woodford, S. C. Main, D. Pallett, and H. Barker (1996) The role of volunteer potatoes in spread of potato virus YN in ware crops of cv. Record. Ann. Appl. Biol. 129: 471-478 https://doi.org/10.1111/j.1744-7348.1996.tb05769.x
  5. De Bokx, J. A. and H. Huttinga (1981) Descriptions of plant viruses. Commonw. Mycol. Inst. Assoc. 242
  6. Cockerham, G. (1943) Potato breeding for virus resistance. Ann. Appl. Biol. 30: 105-108 https://doi.org/10.1111/j.1744-7348.1943.tb06166.x
  7. Fernandez-Northcote, E. N. (1983) Prospects for stability of resistance to potato virus Y. In Research for the Potato in the Year 2000 (Hooker, W. J. ed.). Lima: International Potato Center. pp. 82
  8. Ross, H. (1952) Studies on mosaic resistance in the potato. In Proceedings of the 1951 Conference on Potato Virus Diseases. Wageningen-Lisse, The Netherlands. pp. 40-47
  9. Adams, S. E., R. A. C. Jones, and R. H. A. Coutts (1986) Expression of potato virus X resistant gene Rx in potato leaf protoplasts. J. Gen. Virol. 67: 2341-2345 https://doi.org/10.1099/0022-1317-67-11-2341
  10. Jones, R. A. (1990) Strain group specific and virus specific hypersensitive reactions to infection with potyviruses in potato cultivars. Ann. Appl. Biol. 117: 93-105 https://doi.org/10.1111/j.1744-7348.1990.tb04198.x
  11. Roh, K. S. and B. Y. Choi (2004) Sucrose regulates growth and activation of rubisco in tobacco leaves in vitro. Biotechnol. Bioprocess Eng. 9: 229-235 https://doi.org/10.1007/BF02942298
  12. Park, J. P., S. J. Lee, T. J. Park, K. B. Lee, I. S. Choi, S. Y. Lee, M. G. Kim, and B. H. Chung (2004) Microcontact printing of biotin for selective immobilization of streptavidin- fused proteins and SPR analysis. Biotechnol. Bio process Eng. 9: 137-142 https://doi.org/10.1007/BF02932997
  13. Barker, H. and B. D. Harrison (1984) Expression of genes for resistance to potato virus Y in potato plants and protoplasts. Ann. Appl. Biol. 105: 539-545 https://doi.org/10.1111/j.1744-7348.1984.tb03080.x
  14. Fang, Y. L., S. P. Dhital, K. H. Li, D. M. Khu, H. Y. Kim, Y. S. Song, and H. T. Lim (2005) Utilization of single nodal cuttings and therapies for eradicating doubleinfected potato virus (PLRV, PVY) from in vitro plantlets of potato (Solanum tuberosum). J. Kor. Soc. Hort. Sci. 46: 119-125
  15. Nie, X. and R. P. Singh (2001) A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tuvers. J. Virol. Methods 91: 37-49 https://doi.org/10.1016/S0166-0934(00)00242-1
  16. Myslik, J. T. and A. Nassuth (2001) Rapid detection of viruses, transgenes, and mRNAs in small plant leaf samples. Plant Mol. Biol. Rep. 19: 329-340 https://doi.org/10.1007/BF02772831
  17. Andre, N. D., C. Cesar, C. T. Antonio, and C. A. Antonio (2001) Resistance levels to two strains of Potato virus Y (PVY) in transgenic potatoes cv. Achat. Hortic. Bras. 19: 348-350 https://doi.org/10.1590/S0102-05362001000300012
  18. Horne, R. W. and P. Wildy (1963) Virus structure revealed by negative staining. Adv. Virus Res. 10: 101-170
  19. Mori, K., R. Iriye, M. Hirata, and K. Takamizawa (2004) Quantification of Bacillus species in a wastewater treatment system by the molecular analyses. Biotechnol. Bioprocess Eng. 9: 482-489 https://doi.org/10.1007/BF02933490
  20. Vidal, S., H. Cabrera, R. A. Andersson, A. Fredriksson, and J. P. Valkonen (2002) Potato gene Y-1 is an N gene homolog that confers cell death upon infection with potato virus Y. MPMI 15: 717-727 https://doi.org/10.1094/MPMI.2002.15.7.717
  21. Hamalainen, J. H., V. A. Sorri, K. N. Watanable, C. Gebhardt, and J. P. T. Valkonen (1998) Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato. Theor. Appl. Genet. 96: 1036-1043 https://doi.org/10.1007/s001220050836
  22. Jones, J. B., J. P. Jones, R. E. Stall, and T. A. Zitter (1991) Compendium of Tomato Diseases. pp. 42-43. American Phytopathological Society, St. Paul, Minnesota, USA
  23. Bendahmane, A., K. Kanyuka, and D. C. Baulcombe (1999) The Rx gene from potato controls separate virus resistance and cell death responses. Plant Cell 11: 781- 791 https://doi.org/10.1105/tpc.11.5.781
  24. Hinrichs-Berger, J., M. Harfold, S. Berger, and H. Buchenauer (1999) Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y. Physiol. Mol. Plant Pathol. 55: 143-150 https://doi.org/10.1006/pmpp.1999.0216
  25. Kosslak, R. M., M. A. Chamberlin, R. G. Palmer, and B. A. Bowen (1997) Programmed cell death in root cortex of soybean root necrosis mutants. Plant J. 11: 729-745 https://doi.org/10.1046/j.1365-313X.1997.11040729.x
  26. Barker, H. (1996) Inheritance of resistance to potato viruses Y and A in progeny obtained from potato cultivars containing gene Ry: evidence for new gene for extreme resistance to PVA. Theor. Appl. Genet. 93: 710- 716 https://doi.org/10.1007/BF00224066
  27. Brigneti, G., J. Garcia-Mass, and D. C. Baulcombe (1997) Molecular mapping of potato virus Y restance gene (Rysto) in potato. Theor. Appl. Genet. 94: 198-203 https://doi.org/10.1007/s001220050400
  28. Hamalainen, J. H., K. N. Watanable, J. P. T. Valkonen, A. Arihara, R. L. Plaisted, E. Pehu, L. Miller, and S. A. Slack (1997) Mapping and marker-assisted selection for a gene for extreme resistance potato virus Y. Theor. Appl. Genet. 94: 192-197 https://doi.org/10.1007/s001220050399
  29. Gebhardt, C. and J. P. Valkonen (2001) Organization of genes controlling disease resistance in the potato genome. Annu. Rev. Phytopathol. 39: 79-102 https://doi.org/10.1146/annurev.phyto.39.1.79
  30. Leister, D., A. Ballvora, F. Salamini, and C. Gebhardt (1996) A PCR based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nat. Genet. 14: 421-429 https://doi.org/10.1038/ng1296-421
  31. Yi, J. Y., H. W. Seo, S. W. Lee, M. S. Yang, and S. Y. Kim (2003) NPTII protein expression and resistance to bacterial disease in transgenic potato with lytic gene. J. Kor. Soc. Hort. Sci. 44: 187-191