International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Identification of Enterococcus faecalis on MSB Medium Selective for Mutans Streptococci

  • Lee, Seung-Hoon (Department of Orthodontics, College of Dentistry, Chosun University) ;
  • Yoo, So-Young (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kim, Hwa-Sook (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kang, Sook-Jin (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Lim, Sung-Hoon (Department of Orthodontics, College of Dentistry, Chosun University) ;
  • Kim, Kwang-Won (Department of Orthodontics, College of Dentistry, Chosun University) ;
  • Park, Jung-Min (Department of Korean Culture Center of Microorganisms(KCCM)) ;
  • Shin, Yong-Kook (Department of Chungbuk Bio Industry Foundation, Health Industry Center) ;
  • Shin, Jeong-Hwan (Department of Laboratory Medicine Busan Paik Hospital, College of Medicine, Inje University) ;
  • Baek, Dong-Heon (Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University) ;
  • Choe, Son-Jin (Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University)
  • Published : 2006.03.30
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Abstract

Mitis-salivarius sucrose bacitracin(MSB) medium is widely used in the selective isolation of mutans streptococci(MS), a designation for a group of oral cariogenic species. Recently, we have isolated three bacterial strains grown on MSB agar from human dental plaques. The three strains exhibited biochemical characteristics similar to those of the biotype IV of MS, with the exception that they manifested a positive reaction for arginine deaminase. The objective of this study was to identify and characterize these three clinical isolates. The bacteria were identified with biochemical tests as well as by 16S rDNA cloning and sequencing. In order to compare the antibiotics susceptibility of the clinical isolates with that of type strain, the minimum inhibitory concentrations of 9 antibiotics were determined using broth dilution assays. The results identified all of our three clinical isolates as Enterococcus faecalis. All E. faecalis strains were found to be susceptible to penicillin G, amoxicillin, augmentin, and vancomycin, but were resistant to ciprofloxacin, cefuroxim axetil, and clindamycin. Our findings indicate that E. faecalis is capable of growing on MSB agar, and suggest that the MSB medium be improved so that only MS should be recoverable on the medium, as originally devised for their selection.

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References

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