International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지

Effects of Interleukin-$1\beta$, Tumor Necrosis Factor-$\alpha$ and Interferon-$\gamma$ on the Nitric Oxide Production and Osteoclast Generation in the Culture of Mouse Bone Marrow Cells

  • Kwon, Young-Man (Dept. of Oral Biochemistry, Dankook University) ;
  • Kim, Se-Won (Dept. of Dental Pharmacology, Dankook University) ;
  • Ko, Seon-Yle (Dept. of Oral Biochemistry, Dankook University)
  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of $30{\mu}M$ SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration($3{\mu}M$) of SNP enhanced it. Although the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone metabolism and bone pathophysiology.

Keywords

References

  1. Anderson, D.M., Maraskovsky, E., Billingsley, W.L.: Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F, Cosman, D. and Galibert, L.:A homologue of the TNF receptor and its ligand enhance T-cell growth and dendriticcell function. Nature 390: 175-179, 1997 https://doi.org/10.1038/36593
  2. Azuma, Y., Kaji, K., Katogi, R., Takeshita, S. and Kudo, A.: Tumor necrosis factor-alpha induces differentiation of and bone resorption by osteoclasts. J. Cell. Biol. 275: 4858-4864, 2000
  3. Barman, S.A.: Effect of nitric oxide on mitogen-activated protein kinases in neonatal pulmonary vascular smooth muscle. Lung 183: 325-335, 2005 https://doi.org/10.1007/s00408-005-2545-4
  4. Bertolini, D.R., Nedwin, G.E., Bringman, T.S., Smith, D.D. and Mundy, G.R.: Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature 319: 516-518, 1986 https://doi.org/10.1038/319516a0
  5. Cenci, S., Weitzmann, M.N., Roggia, C., Namba, N., Novack, D., Woodring, land Pacifici, R.: Estrogen deficiency induces bone loss by enhancing T-cell production of TNFalpha. J Clin. Invest. 106: 1229-1237, 2000 https://doi.org/10.1172/JCI11066
  6. Cohen, R.I., Wilson, D. and Liu, S.F: Nitric oxide modifies the sarcoplasmic reticular calcium release channel in endotoxemia by both guanosine-3',5'(cyclic) phosphate-dependent and independent pathways. Crit. Care. Med. 34:173-181, 2006 https://doi.org/10.1097/01.CCM.0000194722.12260.F9
  7. Collin-Osdoby, P., Nickols, G.A. and Osdoby, P.: Bone cell function, regulation, and communication: A role for nitric oxide. J. Cell. Biochem. 57: 399-408, 1995 https://doi.org/10.1002/jcb.240570305
  8. Collin-Osdoby, P., Rothe, L., Anderson, F., Nelson, M., Maloney, W. and Osdoby, P.: RANKL and OPG expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis. J. Biol. Chem. 276: 20659-20672, 2001 https://doi.org/10.1074/jbc.M010153200
  9. Dewhirst, F.E., Stashenko, P. P., Mole, J.E. and Tsurumachi, T.: Purification and partial sequence of human osteoclastactivating factor: Identity with interleukin 1$\beta$. J. Immunol. 135:2562-2568, 1985
  10. Ding, A., Nathan, C.F. and Stuehr, D.J.: Release of reactive nitrogen intermediates and reacrive oxygen intermediate from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 141: 2407, 1988
  11. Fan, X., Roy, E., Zhu, L., Murphy, T.C., Ackert-Bicknell, C., Hart, C.M., Rosen, C., Nanes, M.S. and Rubin, J.: Nitric oxide regulates RANKL and OPG expression in bone stromal cells, Endocrinology 145: 1-9, 2004 https://doi.org/10.1210/en.2003-1225
  12. Freeman, B.: Free radical chemistry of nitric oxide. Chest 105(suppl): 79s-84s, 1994 https://doi.org/10.1378/chest.105.3_Supplement.79S
  13. Gowen, M., Wood, D.D., Ihrie, E.J, McGuire, M.K.B. and Russel, R.GG: An interleukin-1 like factor stimulates bone resorption in vitro. Nature 306: 378-380, 1983 https://doi.org/10.1038/306378a0
  14. Hibbs, J.B., Traintor, R.R., Vavrin, Z. and Rachlin, E.M.: Nitric oxide: A cytotoxic activated macrophage effector molecule. Biochem. Biophys. Res. Commun. 157: 87-94, 1988 https://doi.org/10.1016/S0006-291X(88)80015-9
  15. Hofbauer, L.C.: Osteoprotegerin ligand and osteoprotegerin: novel implications for osteoclast biology and bone metabolism. Eur. J Endocrinol. 141:195-210,1999 https://doi.org/10.1530/eje.0.1410195
  16. Howard, G,A.: Basic and clinical research, in Normal and abnormal bone growth, edited by Dixon, A.D. and Sarnat, B.G, pp 67-76, New York, Alan RLiss, 1985
  17. Ignarro, L.J.: Nitric oxide: A novel signal transduction mechanism for transcellular communication. Hypertension 16:477-483, 1990 https://doi.org/10.1161/01.HYP.16.5.477
  18. Kanematsu, M., Sato, T, Takai, H., Watanabe, K., Ikeda, K. and Yamada, Y.: Prostaglandin E2 induces expression of receptor activator of nuclear factor-kappa B ligand on pre-B cells: implicationf for accelerated osteoclastogenesis in estrogen defiency. J. Bone Miner. Res. 15: 1321-1329, 2000 https://doi.org/10.1359/jbmr.2000.15.7.1321
  19. Kasten, T.P., Collin-Osdoby, P., Patel, N., Osdoby, P., Siegel, N.R., Misko, TP., Currie, M.G and Nickols, GA.: Potentiation of osteoclastic bone resorbing activity by inhibition of nitric oxide synthase. Proc. Natl. Acad. Sci. USA. 91: 3569-3573,1994
  20. Kobayashi, K., Takahashi, N., Jimi, E., Udagawa, N., Takami, m., Kotake, S., Nakagawa, N., Kinisaki, M., Yamaguchi, K., Shima, N., Yasuda, H., Morinaga, T., Higashino, K., Martin, T.J. and Suda, T.: Tumor necosis factor alpha stimulates osteoclast differentiation by a mechanism independent of the ODF/RANKL-RANK interaction. J. Exp. Med. 191:275-286, 2000 https://doi.org/10.1084/jem.191.2.275
  21. Kwak, H.J., Song, J.S., No, Z.S., Song, J.H., Yang, S.D. and Cheon, H.G: The inhibitory effects of roflumilast on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells are mediated by heme oxygenase-1 and its product carbon monoxide. Inflamm Res. 54: 508-513, 2005 https://doi.org/10.1007/s00011-005-1386-1
  22. Lowenstein, C.J. and Snyder, S.H.: Nitric oxide, a novel biological messenger. Cell 70: 705-707. 1992 https://doi.org/10.1016/0092-8674(92)90301-R
  23. Lowik, W.G.M.C., Nibbering, P.H., Van der Ruit, M. and Papapoulos, S.E.: Inducible production of nitric oxide in osteoblast-like cells and in fetal mouse bone explants is associated with suppression of osteoclastic bone resorption. J. Clin. Invest. 93: 1465-1472, 1994 https://doi.org/10.1172/JCI117124
  24. MacIntyre, I., Zaidi, M., Towhidul, Alam, A.S.M., Datta, H.K., Moonga, B.S., Kidbury, P.S., Hecker, M. and Vane, J.M.: Osteoclast inhibition: An action of nitric oxide not mediated by cyclic GMP. Proc. Natl. Acad. Sci. USA 88: 2936-2940,1991
  25. McCall, T.R, Boughton-Smith, N.K., Palmer, R.M.J., Whittle, R.J.R. and Moncada, S.: Synthesis of nitric oxide from Larginine by neutrophils, Biochem. J. 261: 293-296, 1989 https://doi.org/10.1042/bj2610293
  26. Minkin, C.: Bone acid phosphatase: tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif. Tissue Int. 34:285-290, 1982 https://doi.org/10.1007/BF02411252
  27. Mundy, G.R. and Roodman, G.D.: Osteoclast ontogeny and function, In Bone and Mineral Research, vol. 5, edited by Peck, W.A, pp 209-279, Elsevier, Amsterdam, 1987
  28. Nicholson, A.G, Haites, N.E., Mckay, N.G, Wilson, H.M., MacLeod, A.M. and Benjamin, N.: Induction of nitric oxide synthase in human mesangial cells. Biochem. Biophys. Res. Commun. 193: 1269-1274, 1993 https://doi.org/10.1006/bbrc.1993.1762
  29. Nussler, A.K, DiSilvio, M., Billiar, T.R., Hoffman, R.A, Geller D.A, Selby, R., Madrigas, J. and Simmons, R.L.: Stimulation of the nitric oxide synthase pathway in human hepatocytes by cytokines and endotoxin. J. Exp. Med. 176: 261-264,1992 https://doi.org/10.1084/jem.176.1.261
  30. Palmer, R.M.J., Andrews, T., Foxwell, N.A. and Moncada, S.: Glucocorticoids do not affect the induction of a novel calcium dependent nitric oxide synthase in rabbit chondrocytes. Biochem. Biophys. Res. Commun. 188: 209-215,1992 https://doi.org/10.1016/0006-291X(92)92371-4
  31. Punjabi, C.J., Laskin, D.L., Heck, D.E. and Laskin, J.D.: Production of nitric oxide by murine bone marrow cells. J. Immunol. 149: 2179-2184, 1992
  32. Raisz, L.G: Hormonal regulation of bone growth and remodeling. in Cell and. Molecular Biology of Vertebrate Hard Tissue CIBA Foundation Symposium 136, pp 226-238, Wiley, New York, 1988
  33. Ralston, S.H., Helfrich, M.H., Grabowski, P.S. and Benjamin, N.: A role for nitric oxide in the regulation of cytokineinduced bone resorption J. Bone Miner. Res. (Suppl 1) 8: 383(abstract), 1993
  34. Ralston, S.H., Ho, L.P., Helfrich, M.H., Grabowski, P.S., Johnston, P.W. and Benjamin, N.: Nitric oxide: A cytokineinduced regulator of bone resorption J. Bone Miner. Res. 10: 1040-1049, 1995 https://doi.org/10.1002/jbmr.5650100708
  35. Romas, E., Sims, N.A., Hards, D.K., Lindsay, M., Quinn, J.W., Ryan, P.E, Dunstan, C.R., Martin, T.J. and Gillespie, M.T.: Osteoprotegerin reduces osteoclast numbers and prevents bone erosion in collagen-induced arthritis. Am. J. Pathol. 161 :1419-1427, 2002 https://doi.org/10.1016/S0002-9440(10)64417-3
  36. Rubin, J., Murphy, T.C., Zhu, L., Roy, E., Nanes, M.S. and Fan, X: Mechanical strain differentially regulates endothelial nitric-oxide synthase and receptor activator of nuclear kappa B ligand expression via ERKl/2 MAPK. J. Biol. Chem. 278: 34018?34025, 2003 https://doi.org/10.1074/jbc.M302822200
  37. Scheven, B.A.A., Visser, J.W.M. and Nijweide, P.J.: In vitro osteoclast generation from different bone marrow fractions, including a highly enriched hematopoietic stem cell population. Nature 321: 79-81, 1986 https://doi.org/10.1038/321079a0
  38. Srivastava, S., Toraldo, G, Weitzmann, M.N., Cenci, S., Ross, E.P. and Pacifici, R.: Estrogen decreases osteoclast formation by down-regulating receptor activator of NF-kappaB ligand(RANKL)-induced JNK activation. J. Biol. Chem. 276:8836-8840,2001 https://doi.org/10.1074/jbc.M010764200
  39. Suda, T., Takahashi, N. and Martin, T.J.: Modulation of osteoclast differentiation. Endocrine. Rev. 13: 66-80, 1992
  40. Stefanovic-Racic, M., Stadler, J. and Evans, C.H.: Nitric oxide and arthritis. Arth. Rheum. 36: 1036-1044, 1993 https://doi.org/10.1002/art.1780360803
  41. Stem, P.H. and Diamond, J.: Sodium nitroprusside increases cyclic GMP in fetal rat bone cells and inhibits resorption of fetal rat limb bone. Res. Commun. Chem. Pathol. Pharmacol. 75: 19-27, 1992
  42. Tatakis, D.N.: Interleukin-l bone metabolism: A review. J. Periodontol. 64: 416-431,1993
  43. Thompson, B.M., Mundy, G.R. and Chambers, T.J.: Tumor necrosis factors $\alpha$ and $\beta$ induce osteoblastic cells to stimulate osteoclastic bone resorption. J. Immunol. 138: 775-779, 1987
  44. Thomson, B.M., Saklatvala, J. and Chambers, T.J.: Osteoblasts mediate interleukin 1 stimulation of bone resorption by rat osteoclast. J. Exp. Med. 164: 104-112, 1986 https://doi.org/10.1084/jem.164.1.104
  45. van't Hof, R.J. and Ralston, S.H.: Nitric oxide and bone, Immunology 103:255-261,2001 https://doi.org/10.1046/j.1365-2567.2001.01261.x
  46. Zaidi, M., Alam, A.S., Bax, B.E., Shankar, V.S., Bax, C.M., Gill, J.S., Pazianas, M., Huang, C.L., Sahinoglu, T., Moonga, B.S., Stevens, C.R. and Blake, D.R.: Role of the endothelial cell in osteoclast control: New perspectives. Bone 14: 97102, 1993