Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

Spaciotemporal Plasticity of Intergeniculate Leaflet Using Genetically Modified Pseudorabies Virus Recombinant

유전자 조작된 Pseudorabies Virus 변종을 이용한 무릎사이작은핵의 시.공간적 가소성

  • 김진상 (대구대학교 물리치료학과) ;
  • 박은세 (대구대학교 물리치료학과) ;
  • 천송희 (대구대학교 물리치료학과) ;
  • 김민희 (대구대학교 물리치료학과) ;
  • 방현수 (대구대학교 물리치료학과) ;
  • 권영실 (대구대학교 물리치료학과) ;
  • 이봉희 (제주대학교 의과대학 해부학교실) ;
  • 김영철 (계명대학교 공중보건학과)
  • Published : 2006.12.30
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Abstract

This study was carried out to investigate the spaciotemporal plasticity of intergeniculate leaflet in postnatal mongolian gerbil using genetically modified pseudorabies virus recombinant, which was a kind of excellent neurotracer with the ability to transpass the neuronal synaptic cleft. In addition, we tried to evaluate the special role of intergeniculate leaflet as a signal controler of circardian rhythm by expression of various nourotransmitters in suprachiasrnatic nucleus. The PRV-BaBlu, a genetically modified strain of PRV-Bartha with lac-Z gene, was injected into vitreous body of postnatal mongolian gerbil, and immunostained. The PRV-Bablu infected the neurons in intergeniculate leaflet of postnatal mongolian gerbil, and the degree of viral infection in postnatal period of experimental animals had tendency to increase with time consuming. This results showed that the mutant PRV-Bar-tha strain with lac-Z gene, PRV-BaBlu, was a very excellent neurotracer to localize the retinogeniculate tract with infection of neurons in intergeniculate leaflet specially.

Keywords

References

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