Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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The Effects of Diallyl Disulfide on Antimetastatic Potential of B16-F10 Murine Melanoma Cells

B16-F10 Murine Melanoma 세포의 암전이 억제에 미치는 Diallyl Disulfide의 효과

  • Kang, Mi-Kyung (Department of Toxicological Research, National Institute of Toxicological Research) ;
  • Jun, Hye-Seung (Department of Toxicological Research, National Institute of Toxicological Research) ;
  • Yum, Yung-Na (Department of Toxicological Research, National Institute of Toxicological Research) ;
  • Hwang, Myung-Sil (Department of Toxicological Research, National Institute of Toxicological Research) ;
  • Park, Mi-Sun (Department of Toxicological Research, National Institute of Toxicological Research) ;
  • Kim, Ok-Hee (Busan Regional Korea FDA)
  • 강미경 (국립독성연구원 독성연구부) ;
  • 전혜승 (국립독성연구원 독성연구부) ;
  • 염영나 (국립독성연구원 독성연구부) ;
  • 황명실 (국립독성연구원 독성연구부) ;
  • 박미선 (국립독성연구원 독성연구부) ;
  • 김옥희 (부산지방식품의약품안전청)
  • Published : 2006.12.30
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Abstract

Diallyl disulfide (DADS), an oil-soluble organosulfur compound in garlic has been reported to suppress tumor growth and to induce apoptosis in cancer. In the present study, we investigated the effects of DADS on pulmonary metastasis of B16-F10 murine melanoma cells. DADS (i.p. 40 mg/kg) significantly (p<0.05) reduced the number of pulmonary metastatic nodules (48%) in experimental pulmonary metastasis assay. We also found that DADS inhibited adhesion, invasion and migration of B16-F10 melanoma cells in a dose-dependent manner. To study the antimetastatic potential of DADS, we performed the effects of DADS on matrix metalloproteinase activity. DADS significantly inhibited the expression of matrix metalloproteinase-2 activity in B16-F10 cells by gelatin zymography. These results suggest that DADS prevent metastasis in part through suppression of migration of B16-F10 melanoma cells by Inhibiting matrix metalloproteirase-2 responsible for degradation of extracellar matrix.

Keywords

References

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