Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effects of Oxytocin and $IL-1{\alpha}$ In Vitro Development of Bovine Embryos Cultured with Uterine Cells

  • Shin, Seung-Oh (College of Animal Life Science, Kangwon National University) ;
  • Park, Soo-Bong (Dairy Cattle Research Division, National Livestock Research Institute, RDA) ;
  • Lee, Dong-Seok (College of Animal Life Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science, Kangwon National University)
  • Published : 2006.12.31
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Abstract

The purpose of this study was to determine effects of oxytocin and $interleukin-1{\alpha}$ on in vitro development of bovine embryo cultured with endometrial epithelial and stromal cells isolated from bovine uterus. The expressions of COX-2 mRNA in bovine endometrium were also studied. When embryos were cultured with epithelial cells, the rate of blastocysts was significantly (p<0.05) higher in embryos treated with oxytocin than that of control group. The rate of hatched blastocysts was also significantly (p<0.05) higher in embryos treated with oxytocin than those of two control groups. On the other hand, when the embryos were cultured with stromal cells, the rate of blastocysts were significantly (p<0.05) higher than those of groups treated with $IL-1{\alpha}$, oxytocin and control with stromal cells than that of control group without stromal cells. The rate of blastocysts hatched were also significantly (p<0.05) higher in group treated with $IL-1{\alpha}$ than those of control group without stromal cells and oxytocin group. In another experiment, COX-2 gene was expressed in embryo group treated with oxytocin during the co-culture of embryos with epithelial cells. In contrast, COX-2 mRNA was expressed in group treated with $IL-1{\alpha}$ when the embryos were cultured with stromal cell. This result shows that oxytocin and $IL-1{\alpha}$ were stimulate embryo development in vitro when embryos were cultured with epithelial and stromal cells, and can affect the development of bovine embryos in the uterus.

Keywords

References

  1. Alan K Goff (2004): Steroid hormone modulation of prostaglandin secretion in the ruminant endometrium during the estrous cycle. BioI Reprod 71:11-16 https://doi.org/10.1095/biolreprod.103.025890
  2. Andrew L, Daniel DC (1993): Uterine epithelial cell secretion of literleukin-1a induced prostaglandin $E_{2}$ ($PGE_{2}$) and $PGF_{2a}$ secretion by uterine stromal cells in vitro. Enocrinology 132:300-308 https://doi.org/10.1210/en.132.1.300
  3. Arosh JA, Parent J, Chapdelaine P, Sirois J, Fortier MA (2002): Expression of cyclooxygenases 1 and 2 and prostaglandin E synthase in bovine endometrial tissue during the oestrus cycle. BioI Reprod 67:161-169 https://doi.org/10.1095/biolreprod67.1.161
  4. Brent MB, Thoma GK (1995): Interleukin-1a regulates prostaglandin poduction and cyclooxygnase activity in sensitized rat endometrial stromal cells in vitro. BioI Reprod 53:126-132 https://doi.org/10.1095/biolreprod53.1.126
  5. Crofford LJ (2001): Prostaglandin biology. Gastroenterol Clin North Am 30:863-876 https://doi.org/10.1016/S0889-8553(05)70217-X
  6. Dinarello CA (1989): Interleukin-1 and it's biologically related cytokines. Adv Immunol 44:153-205 https://doi.org/10.1016/S0065-2776(08)60642-2
  7. Dubois RN, Abramson SB, Crofford L, Gupta RA, Simon LS, Van De Putte LB Lipsky PE (1998): Cyclooxygenase in biology and disease. FASEB Journal 12:1063-1073 https://doi.org/10.1096/fasebj.12.12.1063
  8. Emond V, Murphy BD, Fortier MA, Iamber RD, (1998): Prostaglandin $E_{2}$regulateds both interleukin-2 and granul oocyte-macrophage colony-stimulating factor gene expression in bovine lymphocytes. BioI Reprod 58:143-151 https://doi.org/10.1095/biolreprod58.1.143
  9. Flint APF, Lanning GE, stewart HJ, Abayasekara DRE (1994): The Role of the endometrial oxytocin receptor in determining the length of the sterile oestrous cycle and. ensuring maintenance of luteal function in early pregnancy in ruminants. Phiolosophical Transactions: Biol Sci 344:291-304 https://doi.org/10.1098/rstb.1994.0067
  10. Fortier MA, Guilbault LA, Grasso F (1988): Specific properties of epithelial and stromal cells from the endometrium of cows: J Reprod Fertil 83:239-248 https://doi.org/10.1530/jrf.0.0830239
  11. Gurevich M, Shemesh M (1996): Induction of cyclooxygenase and prostaglandin $E_{2}$ production by the bovine pre-embryo. Reprod Fertil 6:687-691
  12. Kennedy TG (1980): Prostaglandins and the endometrial vascular permeability changes preceding blastocyst implantation and decidualization. Prog Reprod BioI 7: 234-243
  13. Keys JL, King GJ, Kennedy TG (1985): Increased uterine vascular permeability at the time of embryonic attachment in the pig. Biol Reprod 34: 405-411 https://doi.org/10.1095/biolreprod34.2.405
  14. Lewis GS, Waterman RA (1985): Metabolism of arachidonic acid in vitro by ovine conceptuses recovered during early pregnancy. Prostaglandins 30: 263-283 https://doi.org/10.1016/0090-6980(85)90190-X
  15. McCracken JA, Custer EE, Lamsa JC (1999): Luteolysis: a neuroendocrine mediated event. Physiol Rev 79:263-323 https://doi.org/10.1152/physrev.1999.79.2.263
  16. Miyamoto Y, Skarzynski DJ, Okuda K (2000): Is tumor necrosis factor a trigger for the initiation of endometrial prostaglandin $F_{2a}$ release at luteolysis in cattle. Biol Reprod 62:1109-1115 https://doi.org/10.1095/biolreprod62.5.1109
  17. Parent F, Villeneuve C, Fortier MA (2003): Evaluation of the contribution of cyclooxygenase 1 and cyclooxygenase 2 to the production of $PGE_{2}$ and $PGF_{2a}$ in epithelial cells form bovine endometrium. Reproducion 126:539-547
  18. Roberts JS, McCracken JA, Gavagan JE, Soloff MS (1976): Oxytocin-stimulated release of prostaglandin F2alpha from ovine endometrium in vitro: correlation with estrous cycle and oxytocin-receptor binding. Endocrinology 99:1107-1114 https://doi.org/10.1210/endo-99-4-1107
  19. Sayre BL, Lewis GS (1993): Arachidonic acid metabolism during early development of ovine embryos: a possible relationship to shedding of the zona pellucida. Prostaglandins 45:557-569 https://doi.org/10.1016/0090-6980(93)90019-4
  20. Smith EM, Anthony F, Masson GM (1991): Follicular fluid prostaglandin $E_{2}$: prostaglandin $F_{2a}$ ratio in relation to outcome of matched oocyte. Hum Reprod 6:519-523 https://doi.org/10.1093/oxfordjournals.humrep.a137371
  21. Soto P, Natzke RP, Hansen PJ (2003): Identification of possible mediators of embryonic mortality caused by mastitis: actions of lipopolysaccharide, prostaglandin $F_{2a}$, and the nitric oxide generator, sodium nitroprusside dihydrate, on oocyte maturation and embryonic development in cattle. Am J Reprod Immunol 50:263-272 https://doi.org/10.1034/j.1600-0897.2003.00085.x
  22. Viggiano JM, Herrero MB, Cegral E, Boquet MG, de Gimeno MF (1995): Prostaglandin synthesis by cumulus-oocyte complexes: effects on in vitro fertilization in mice. Prostag Leukot Essent Fatty Acids 53:261-265 https://doi.org/10.1016/0952-3278(95)90125-6
  23. Yee GM, Squires PM, Cejic SS, Kennedy TG (1993): Lipid mediators of implantation and decidualitation. J Lipid Mediators 6:525