Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지

Distribution of Antennal Sensilla in Cotesia plutellae and Effect of Flagellectomy on Parasitism

프루텔고치벌(Cotesia plutellae)의 촉각 감각기 분포와 편절제거가 기생력에 미치는 영향

  • Kim, Yong-Gyun (School of Bioresource Sciences, Andong National University) ;
  • Park, Jung-A (School of Bioresource Sciences, Andong National University)
  • 김용균 (안동대학교 생명자원과학부) ;
  • 박정아 (안동대학교 생명자원과학부)
  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

A solitary endoparasitoid, Cotesia plutellae, parasitizes diamondback moth, Plutellae xylostella. It has been suggested that its antennae are a major sensory organ to search host and recognize host developmental and other physiological condition during parasitization. This research was performed to understand the parasitic behavior of C. plutellae by analyzing sensory types and their numbers on the antennae using scanning electron microscope. There was no significant difference in antennal length in both male and female C. plutellae, in which both sexes had 16 flagellomeres. Three different types of sensilla (trichoid, seta, and placodea sensilla) were located mostly on flagella and analyzed in their density on the different antennomeres. Trichoid sensillum was the major sensory type and showed about 87% density among all sensilla. Both trichoid and placodea types of sensilla exhibited even numbers on all flagellomeres with some decrease at terminal segments. In contrast, seta form of sensilla showed drastic increase in its density at distal part after 9th flagellomere. When distal half of flagellomeres were cut off, the C. plutellae could not parasitize host larvae. Even when only four distal flagellomeres were removed, the parasitism showed only 30%. These results indicate that C. plutellae antennae are required for parasitism and suggest that seta form of sensilla may play significant roles in recognizing host for parasitization.

프루텔고치벌(Cotesia plutellae)은 단독형 내부기생봉으로 배추좀나방(Plutella xylostella)을 기주로 생활한다. 이 기생봉이 배추좀나방을 탐색하고 기주의 발육상태 및 다른 생리 상태를 인식하는데 촉각은 매우 중요한 감각기관으로 작용할 것으로 주목 받아왔다. 본 연구는 이 기생봉의 촉각 구조를 주사전자현미경으로 관찰하고, 관찰된 감각기 종류와 밀도를 바탕으로 기생생리를 이해하고자 수행되었다. 촉각 전체 길이에서 암수간 차이가 없으며 모두 16개 편절마디를 가지고 있다. 감각기 구조는 크게 긴털형감각기, 센털형감각기 그리고 판상감각기로 나누어 밀도조사를 실시하였고, 대부분 감각기는 편절에 집중적으로 분포했다. 이 가운데 긴털형감각기가 암수 모두 약 87%를 차지하였다. 편절에서 긴틸형감각기와 판상감각기는 말단 부위에서 밀도가 감소하는 반면, 센털형감각기는 말단 부위로 갈수록 증가하여, 암컷의 경우 9번째 편절 마디 이후로 급격하게 밀도 증가를 보였다. 촉각 부위별 제거에 따른 기생에 미치는 효과를 분석한 결과 편절 후반부를 제거하면 기생능력을 완전히 상실하고, 말단 4개 편절 마디를 제거해도 불과 30% 이하의 낮은 기생율을 기록했다. 이상의 결과는 프루텔고치벌의 기생에 촉각의 중요성을 보여주었으며, 특히 센털형감각기가 기주 탐색에 밀접히 관여될 가능성을 제시하였다.

Keywords

References

  1. Altner, H. and L. Prillinger. 1980. Ultrastructure of invertebrate chemo-, thermo-, and hygroreceptors and its functional significance. Int. Rev. Cytol. 67: 69-139 https://doi.org/10.1016/S0074-7696(08)62427-4
  2. Bae, S. and Y. Kim. 2004. Host physiological changes due to parasitism of a braconid wasp, Cotesia plutellae, on diamondback moth, Plutella xylostella. Comp. Biochem. Physiol. 138A: 39-44
  3. Basio, N.A. and Y. Kim. 2005. A short review of teratocytes and their characters in Cotesia plutellae (Hymenoptera: Braconidae). J. Asia-Pacific Entomol. 8: 211-217 https://doi.org/10.1016/S1226-8615(08)60093-X
  4. Basio, N.A. and Y. Kim. 2006. Additive effect of teratocyte and calyx fluid from Cotesia plutellae on immunosuppression of Plutella xylostella. Physiol. Entomol. 31: 1-7 https://doi.org/10.1111/j.1365-3032.2005.00487.x
  5. Bleeker, M.A.K., H.M. Smid, A.C. Van Aelst, J.J.A. Van Loon and L.M.E. Vet. 2004. Antennal sensilla of two parasitoid wasps: a comparative scanning electron microscopy study. Microsc. Res. Tech. 63: 266-273 https://doi.org/10.1002/jemt.20038
  6. Boo, KS, Y. Kim, K.C. Park and M.Y. Choi. 2005. Insect hormone and physiology. 875 pp. Seoul National University Press, Seoul
  7. Ibrahim, A.M.A., J.Y. Choi, Y.H. Je and Y. Kim. 2005. Structure and expression profiles of two putative Cotesia plutellae bracovirus genes (CpBV-H4 and CpBV-E94a) in parasitized Plutella xylostella. J. Asia-Pacific Entomol. 8: 359-366 https://doi.org/10.1016/S1226-8615(08)60258-7
  8. Ibrahim, A.M.A. and Y. Kim. 2006. Parasitism by Cotesia plutellae alters the hemocyte population and immunological function of the diamondback moth, Plutella xylostella. J. Insect Physiol. 52: 943-950 https://doi.org/10.1016/j.jinsphys.2006.06.001
  9. Kaeslin, M., R. Pfister-Wilhelm and B. Lanzrein. 2005. Influence of the parasitoid Chelonus inanitus and its polydnavirus on host nutritional physiology and implications for parasitoid development. J. Insect Physiol. 51: 1330-1339 https://doi.org/10.1016/j.jinsphys.2005.08.003
  10. Keil, T.A. 1999. Morphology and development of the peripheral olfactory organs. pp. 5-47. In Insect olfaction, ed. by B.S. Hansson. Springier-Verlag, New York
  11. Kim, Y., N.A. Basio, A.M.A. Ibrahim and S. Bae. 2006. Gene structure of Cotesia plutellae bracovirus (CpBV)-IkB and its expression pattern in diamondback moth, Plutella xylostella, parasitized by C. plutellae. Kor. J. Appl. Entomol. 45: 1-10
  12. Kim, Y. and Y. Son. 2006. Parasitism of Cotesia plutellae alters morphological and biochemical characters of diamondback moth, Plutella xylostella. J. Asia-Pacific Entomol. 9: 37-42 https://doi.org/10.1016/S1226-8615(08)60273-3
  13. Kwon, M., S.H. Lee and M.Y. Lin. 2006. An evidence to use Colesia plutellae (Kurdjumov) (Hymenoptera: Braconidae) as a field control agent against diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae). J. Asia-Pacific Entomol. 9: 55-59 https://doi.org/10.1016/S1226-8615(08)60278-2
  14. Lee, S., N.A. Basio, D. Kim and Y. Kim. 2005. Proteomic analysis of parasitization by Colesia plutellae against diamondback moth, Plutella xylostella. J. Asia-Pacific Entomol. 8: 53-60 https://doi.org/10.1016/S1226-8615(08)60071-0
  15. Lee, S. and Y. Kim. 2004. Juvenile hormone esterase of diamondback moth, Plutella xylostella, and parasitism of Colesia plulellae. J. Asia-Pacific Entomol. 7: 283-287 https://doi.org/10.1016/S1226-8615(08)60228-9
  16. Ochieng, S.A., K.C. Park, J.W. Zhu and T.C. Baker. 2000. Functional morphology of antennal chemoreceptors of parasitoid Microplitis croceipes (Hymenoptera: Braconidae). Archropod Struc. Dev. 29: 231-240 https://doi.org/10.1016/S1467-8039(01)00008-1
  17. Rincon, C., D. Bordat, B. Lohr and S. Dupas. 2006. Reproductive isolation and differentiation between five populations of Cotesia plutellae (Hymenoptera: Braconidae), parasitoid of Plutella xylostella (Lepidoptera: Plutellae). Biol. Control 36: 171-182 https://doi.org/10.1016/j.biocontrol.2005.07.018
  18. Roux, O., J. Van Baaren, C. Gers, L. Arvanitakis and L. Legal. 2005. Antennal structure and oviposition behavior of the Plutella xylostella specialist parasitoid: Cotesia plutellae. Microsc. Res. Tech. 68: 36-44 https://doi.org/10.1002/jemt.20220
  19. Talekar, N.S. and A.M. Shelton. 1993. Biology, ecology, and management of the diamondback moth. Annu. Rev. Entomol. 38: 275-301 https://doi.org/10.1146/annurev.en.38.010193.001423
  20. Van Baaren, J., R. Barbier and J.P. Nenon. 1996. Female antennal sensilla of Epidinocarsis lopezi and Leptomastix dactylopii (Hymenoptera: Encyrtidae), parasitoids of pseudococcid mealybugs. Can. J. Zool. 74: 710-720 https://doi.org/10.1139/z96-080