Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

The Comparison of Morphological and Constituents of the Leaves of Magnolia officinalis, M. biloba and M. obovata

중국 및 일본산 후박 잎의 형태 및 성분 비교

  • Park, Seo-Hee (College of Pharmacy, Chungnam National University) ;
  • Yun, Ui-Jung (College of Pharmacy, Chungnam National University) ;
  • Shin, Jong-Hun (Lab of Marine Natural Products and Structure Determination, Natural Products Research Institute, Seoul National University) ;
  • Kwon, Byoung-Mok (Molecular Cancer Research Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
  • 박서희 (충남대학교 약학대학) ;
  • 윤의중 (충남대학교 약학대학) ;
  • 신종헌 (서울대학교 천연물과학연구소) ;
  • 권병목 (한국생명과학연구소 분자암센터) ;
  • 배기환 (충남대학교 약학대학)
  • Published : 2006.12.30
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Abstract

In this study, we analyzed and quantified the contents of magnolol (1), honokiol (2), and obovatol (3) in the leaves of Magnolia species by HPLC. HPLC methods were developed and validated by ICH guide lines comparing the linearity, intraday precision, inter-day precision. The optimal conditions were as follows; wavelength: 288 nm, column: Zorbax Eclipse XDBC18 $(2.1\times150\;mm,\;5{\mu}m)$, mobile phase: Acetonitril - 0.1 % $H_3PO_4$ (45:55, v/v) buffer, flow rate: 0.5 ml/min. The contents of magnolol, honokiol and obovatol were 1.08%, 2.37%, and 0.57% in the leaves of M. officinalis, 0.50%, 0.03%, and 1.54% in the leaves of M. biloba, 0.37%, 0.03%, and 0.76% in the leaves of M. obovata. The contents of effective ingredients in the leaves of M. officinalis and M biloba were higher than that of M. obovata. Especially, the content of obovatol in the leaves of M. biloba was remarkably high. This result showed that the shapes of leaves have a certain relationship with the content of obovatol.

Keywords

References

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