Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Evaluation of Genetic Diversity among the Genus Viola by RAPD Markers

  • Oh, Boung-Jun (Kumho Life and Environmental Science Laboratory, Korea Kumho Petrochemical Co., Ltd.) ;
  • Ko, Moon-Kyung (Kumho Life and Environmental Science Laboratory, Korea Kumho Petrochemical Co., Ltd.) ;
  • Lee, Cheol-Hee (Dept. of Horticultural Science & Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

The genetic diversity among the genus Viola was evaluated using the random amplified polymorphic DNA (RAPD) method. A total of 142 distinct amplification fragments by 18 random primers were scored to perform the cluster analysis with UPGMA. Viola species from the subsection Patellares were clustered into group I to IV. The groups from I to IV were consistent with its morphological taxonomy, series Pinnatae, Chinensis, Variegatae, and Patellares in the subsection Patellares, respectively. Even though V. albida and V. albida var. takahasii were classified in Chinensis, they were assigned into group I. The cluster analysis separated other subsections from Patellares in the section Nomimium. Interestingly, V. verecunda and V. grypoceras in subsections Biobatae and Trigonocarpae, respectively, were clustered into group C with a high similarity coefficient. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species.

Keywords

References

  1. Abo-elwafa,A.,K.Murai and T. Shimada. 1995. Intra- and Inter-specific variations in Lens revealed by RAPD markers. Theor. Appl. Genet. 90: 335-340
  2. Junghans, H. and M. Metzlaff. 1990. Asimple and rapid method for the preparation of total plant DNA. Biotechnique 8: 176
  3. Kump, B. and B. Javornik. 1996. Evaluation of genetic variability among common buckwheat (Fagopyrum esculetumMoench) populations by RAPD markers. Plant Sci. 114: 149-158 https://doi.org/10.1016/0168-9452(95)04321-7
  4. Lee, C.H. 1993. Viola native to Korea. J. Kor. Flower Res. Soc. 2: 13-22
  5. Lee, S.J., J.S. Shin, K.W. Park and Y.P. Hong. 1996. Detection of genetic diversity using RAPD-PCR and sugar analysis inwatermelon [Citrullus lanantus (Thunb.) Mansf.] germplasm. Theor. Appl.Genet. 92: 719-725 https://doi.org/10.1007/BF00226094
  6. Lee, W.T. and C.S. Yook. 1975. Chemical taxonomy of the genus Viola in Korea. J. Kor. Plant Tax. 6: 1-22 https://doi.org/10.11110/kjpt.1975.6.1.001
  7. Lee, Y.N. and W.C. Lee. 1968. A morphological study of leaf epidermis and seeds on Korean violets. J. Kor. Res. Inst. B. L. 1: 35-39
  8. Rohlf, F.J. 1992. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, Version 1.70. Exeter Software, Setauker, New York, USA
  9. Sharma, S.K., I.K. Dawson and R. Waugh. 1995. Relationships among cultivated and wild lentils revealed by RAPD analysis. Theor. Appl. Genet. 91: 647-654
  10. Sneath, P.M.A. and R.R. Sokal. 1973. Numerical Taxonomy, The principles and practice of numerical classification.W.H. Freedman and Company, San Francisco, USA
  11. Stebbins,G.L., B.L.Harvey, E.L. Cox, J.N. Rutger,G. Jelencovic and E. Yagil. 1963. Identification of the ancestory of an amphiploid Viola with the aid of paper chromatography. Amer. J. Bot. 50: 830-839 https://doi.org/10.2307/2440202
  12. Welsh, J. and M. McClelland. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218 https://doi.org/10.1093/nar/18.24.7213
  13. Wilkie, S.E., P.G. Isaac and R.J. Slater. 1993.Randomamplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor. Appl. Genet. 86: 497-504 https://doi.org/10.1007/BF00838566
  14. William, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S. Tingey. 1990.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic acids Res. 18: 6531-6535 https://doi.org/10.1093/nar/18.22.6531
  15. Woodland, D.W. 1991. Contemporary Plant Systematics. Prentice Hall, New Jersey, USA