Journal of Microbiology

  • 제44권6호
  • /
  • Pages.600-606
  • /
  • 2006
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Phylogenetic Diversity of Acidophilic Sporoactinobacteria Isolated from Various Soils

  • Cho, Sung-Heun (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Han, Ji-Hye (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Seong, Chi-Nam (Department of Biology, College of Natural Sciences, Sunchon National University) ;
  • Kim, Seung-Bum (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University)
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Spore forming actinobacteria (sporoactinobacteria) isolated from soils with an acidic pH in Pinus thunbergii forests and coal mine waste were subjected to taxonomic characterization. For the isolation of acidophilic actinobacteria, acidified starch casein agar (pH adjusted to 4-5) was used. The numbers of actinobacteria growing in acidic media were between $3.2{\times}10^4$ and $8.0{\times}10^6$ CFU/g soil. Forty three acidophilic actinobacterial strains were isolated and their 16S rDNA sequences were determined. The isolates were divided into eight distinctive phylogenetic clusters within the variation encompassed by the family Streptomycetaceae. Four clusters among them were assigned to the genus Streptacidiphilus, whereas the remaining four were assigned to Streptomyces. The clusters belonging to either Streptomyces or Streptacidiphilus did not form a monophyletic clade. The growth pH profiles indicated that the representative isolates grew best between pH 5 and 6. It is evident from this study that acidity has played a critical role in the differentiation of the family Streptomycetaceae, and also that different mechanisms might have resulted in the evolution of two groups, Streptacidiphilus (strict acidophiles) and neutrotolerant acidophilic Streptomyces. The effect of geographic separation was clearly seen among the Streptacidiphilus isolates, which may be a key factor in speciation of the genus.

키워드

참고문헌

  1. Felsenstein, J. 1993. PHYLIP (phylogenetic inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA
  2. Huang, Y., Q. Cui, Li. Wang, C. Rodriguez, E. Quintana, M. Goodfellow, and Z. Liu. 2004. Streptacidiphilus jiangxiensis sp. nov., a novel actinomycete isolated from acidic rhizosphere soil in China. Antonie Leeuwenhoek 86, 159-165 https://doi.org/10.1023/B:ANTO.0000036124.18820.ae
  3. Jukes, T.H. and C.R. Cantor. 1969. Evolution of protein molecules. In Mammalian Protein Metabolism, volume 3, p. 21-213. Edited by H.N. Munro. New York: Academic Press
  4. Kim, S.B., C.N. Seong, S.J. Jeon, K.S. Bae, and M. Goodfellow. 2004. Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov. Int. J. Syst. Evol. Microbiol. 54, 211-214
  5. Kim, S.B., J. Lonsdale, C.N. Seong, and M. Goodfellow. 2003. Streptacidiphilus gen. nov., acidophilic actinomycetes with wall chemotype I and emendation of the family Streptomycetaceae (Waksman and Henrici $(1943)^{AL}$) emend. Rainey et al. 1997. Antonie Van Leeuwenhoek 83, 107-116 https://doi.org/10.1023/A:1023397724023
  6. Lim, Y.W., S.A. Lee, S.B. Kim, H.Y. Yong, S. H. Yeon, Y. K Park, D.W. Jeong, and J.S. Park. 2005. Diversity of denitrifying bacteria isolated from Daejeon sewage treatment plant. J. Microbiol. 43, 383-390
  7. Lonsdale, J.T. 1985. Aspects of the biology of acidophilic actinomycetes, Ph.D. thesis, University of Newcastle, Newcastle upon Tyne, UK
  8. Park, M.S., S.R. Jung, M.S. Lee, K.O. Kim, J.O. Do, K.H. Lee, S.B. Kim, and K.S. Bae. 2005. Isolation and characterization of bacteria associated with two sand dune plant species, Calystegia soldanella and Elymus mollis. J. Microbiol. 43, 219-227
  9. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425
  10. Seong, C.N. 1992. Numerical taxonomy of acidophilic and neutrotolerant actinomycetes isolated from acid soil in Korea. Ph.D. thesis, Seoul National University, Seoul, Korea
  11. Seong, C.N., S.K. Park, M. Goodfellow, S.B. Kim, and Y.C. Hah. 1995. Construction of probability identification matrix and selective medium for acidophilic actinomycetes using numerical classification data. J. Microbiol. 33, 95-102
  12. Wang, L., Y. Huang, Z. Liu, M. Goodfellow, and C. Rodriguez. 2006. Streptacidiphilus oryzae sp. nov., an actinomycete isolated from rice-field soil in Thailand. Int. J. Syst. Evol. Microbiol. 56, 1257-1261 https://doi.org/10.1099/ijs.0.64165-0
  13. Xu, C., L. Wang, Q. Cui, Y. Huang, Z. Liu, G. Zheng, and M. Goodfellow. 2006. Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp. nov. and Streptomyces yanglinensis sp. nov. Int. J. Syst. Evol. Microbiol. 56, 1109-1115 https://doi.org/10.1099/ijs.0.63959-0
  14. Yeon, S.H., W.J. Jeong, and J.S. Park. 2005. The diversity of culturable organotrophic bacteria from local solar salterns. J. Microbiol. 43, 1-10