Journal of Ginseng Research

  • 제30권4호
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  • Pages.206-211
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  • 2006
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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한국내 각지에서 채집된 산삼의 균근(菌根) 형태와 감염율

The Rate and Morphology of Mycorrhizal Infection in the Wild Ginseng (Panax ginseng C.A. Meyer) Collected from Various Locations in Korea

  • 이규화 (서울대학교 농업생명과학대학 산림과학부) ;
  • 이경준 (서울대학교 농업생명과학대학 산림과학부) ;
  • 박훈 ;
  • 발행 : 2006.12.31
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초록

국내에서 채집되는 산삼의 내생균근(Arbuscular Mycor-rhizal Fungi, hMF) 감염을 조사하기 위해 전국 각지에서 생산되는 산삼으로부터 뿌리시료를 채취하였다. 균근감염은 채취한 뿌리를 세척하고 곰팡이의 균사를 frypan blue로 염색하여 관찰하였다. 본 연구에서 산삼의 품질은 12인의 전문가로 구성된 한국산삼협회의 산삼감정단에 의해 감정되었.다. 감정 의뢰된 산삼의 품질은 다섯 가지,즉,천종(순수 자연산삼), 지종(야생 산삼의 씨앗을 자연 상태에 파종하여 생산), 인종(약간의 변경이 가해진 자연환경에 otf산삼의 씨앗이나 모종을 심어서 생산), 품질미달(연령이 10년 미만 또는 병, 상처발생 등),수입 산삼 등 이다. 감염된 균근의 형태는 vesicle이 드물고, 세포 내부에는 arbuscule 대신 hyphal coil이 발견되어 hE?F중에서 Paris-type로서 확인되었다. 본 연구대상 산삼의 평균 균근감염율은 58.3%였으며, 다섯품질 간의 감염율에 큰 차이가 없었다 가는 뿌리에서 채취한 시료를 관찰해본 결과 총길이의 18.7%가 균근에 감염되어 있었다. 경기지방과 해발 1,200m이하에서 채집한 산삼의 균근감염율이 각각 84.2%, 70%로 평균보다 높은 반면,경북지방에서 채집한 산삼의 균근감염율이 27.8%로 낮았다. 연령이 오래된 산삼의 균근감염율이 상대적으로 낮은 것으로 나타났다.

To investigate mycorrhizal infection by arbuscular mycorrhizal fungi(AMF), samples of fine lateral roots were taken from the wild ginseng(Panax ginseng C.A. Meyer) naturally growing at various locations in Korea. Mycorrhiazal infections were studied by cleaning the root samples and staining fungal hyphae with frypan blue. Wild ginsengs for this study were graded by an appraisal committee consisting of 12 experts of Korea Mountain Ginseng Association. Following five quality groups were recognized: Heaven group(pure natural), Earth group (from seeding of wild ginseng), Man group(from seeding or seedlings of wild ginseng with slight environmental modification), unmarketable, and imported wild ginseng. Morphology of AMF was typical Paris-type which shows intracellular hyphal coils with rare vesicles and lack of arbuscules. Average infection rate of individual wild ginsengs was 58.3% and showed no differences among five quality groups. When portions of fine roots were quantified for mycorrhizal infection, 18.7% of the total length of the primary and secondary roots were infected by AMF. Wild ginsengs from Gyeonggi Province(84.2%), and from mountains lower than 1,200 meters above sea level(about 70%) showed higher infection rate, while the ginseng from Gyeongbuk Province(27.8%) had lower rate. Wild ginsengs at older age showed lower infection rates.

키워드

참고문헌

  1. 한국인삼사편찬위원회: 韓國人蔘史. 동일문화사, 서울 (2002)
  2. 김홍대: 한국의 山蔘. 김영사, 파주 경기도 (2005)
  3. 주영승: 산삼의 종류 및 진위감정. 우리 蔘 硏究 창간호, 6-10 (2001)
  4. Park, H., Park, S.M. and Sang, H. : Production and quality of mountain ginseng. Proceedings of the 8th Int. Sym. on Ginseng, Korean Society of Ginseng, 456-466 (2002)
  5. Wang, Z.H., Li, C.H., Zhang, C.G., Wang, X.D. : Study on thewild ginseng and cultivated ginseng in Northeast of China. Proceedings of International Symposium on Ginseng. 109-114 (1998)
  6. 박기홍, 강병수: 인삼의 재배방법에 따른 정유성분에 대한 연구. 동국한의학연구소논문집 2(2), 31-56 (1993)
  7. Smith, S.E. and Read, D.J. : Mycorrhizal Symbiosis, second edition. Academic Press, San Diego, CA. (1997)
  8. Whitebread, F., McGonigle, T.P. and Peterson, R.L. : Vesicular- arbuscular mycorrhizal associations of American ginseng(Panax quinquefolius) in commercial production. Can. J. Bot. 74, 1104-1112 (1996) https://doi.org/10.1139/b96-135
  9. McGonigle, T.P., Hovius, J.P. and Peterson, R.L. : Arbuscular mycorrhizae of American ginseng (Panax quinquefolius L.) in cultivated field plots: plant age affects the development of a colonization lag phase. Can. J. Bot. 77, 1028-1034 (1999) https://doi.org/10.1139/cjb-77-7-1028
  10. Park, H., Lee, M.G., Lee, C.H., Lee, K.J. : Identification of V.A. mycorrhiza in the cultured Panax ginseng. Korean Soc. Soil Sci. Fert. 23(1), 73-76 (1990)
  11. Lee, K.J., Park, H. and Lee, I.S. : Morphology of arbuscular mycorrhizal roots and effects of root age and soil texture on the mycorrhizal infection in Panax ginseng C.A. Meyer. J. Ginseng Res. 28(3), 149-156 (2004) https://doi.org/10.5142/JGR.2004.28.3.149
  12. Han, K.D., Lee, S.S., Kim, S.H., and Lee, M.W. : Effects of beneficial microorganisms and mycorrhizal fungus colonized rhizoplane on the suppression of root rot pathogen, Fusarium solani. Korean J. Mycology 24(1), 38-48 (1996)
  13. Lee, K.J. and Koo, C.D. : Taxonomic distribution of ectoand endomycorrhizae among woody species in Korea. J. Korean For. Soc. 59, 37-45 (1983)
  14. McGonigle, T.P., Miller, M.H., Evans, D.G., Fairchild, G.L. and Swan, J.A. : A new method which gives an objective of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol. 115, 495-501 (1990) https://doi.org/10.1111/j.1469-8137.1990.tb00476.x
  15. Ren, S., Fang, S. and Wang, R. : An ecological distribution of VA Mycorrhizal fungi in ginseng(Panax ginseng C.A. Meyer) in Changbai Mountains area in the Northeast of China. Korean J. Ginseng Sci., 16(3), 241-242 (1992)
  16. Konsler, T.R., Zito, S.W., Shelton, J.E. and Staba, E.J. : Lime and phosphorus effects on American ginseng. II. Root and leaf ginsenoside content and their relationship. Journal of the American Society for Horticultural Science 115(4), 575-580 (1990)
  17. Fournier, A.R., Khanizadeh, S., Gauthier, L., Gosselin, A., and Dorais, M. : Effect of Two Glomus Species Inoculations on Survival, Photosynthetic Capacity, Growth, Morphology and Root Ginsenoside Content of Panax quinquefolius L. J. Ginseng Res. 27(4), 178-182 (2003) https://doi.org/10.5142/JGR.2003.27.4.178
  18. Woo, S.Y., Lee, D.S. and Min, J.K. : A study on the growth and environments of Panax ginseng in the different forest stands. J. Korean For. Soc. 91(3), 304-312 (2002)
  19. Charron, D. and Gagnon, D. : The demography of northern populations of Panax quinquefolium(American ginseng). J. Ecol. 79(2), 431-445 (1991) https://doi.org/10.2307/2260724

피인용 문헌

  1. Effect of Arbuscular Mycorrhizal Fungi on Growth of Korean Ginseng (Panax ginseng C. A. Mey.) Seedlings vol.41, pp.2, 2013, https://doi.org/10.4489/KJM.2013.41.2.81