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Polyphenol Contents and Antioxidant Activity of Lepista nuda

민자주방망이버섯의 폴리페놀 함량과 항산화활성

  • Lee, Yang-Suk (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Joo, Eun-Young (Dept. of Herbal Biotechnology, Daegu Haany University) ;
  • Kim, Nam-Woo (Dept. of Herbal Biotechnology, Daegu Haany University)
  • 이양숙 (대구한의대학교 한방생약자원학과) ;
  • 주은영 (대구한의대학교 한방생약자원학과) ;
  • 김남우 (대구한의대학교 한방생약자원학과)
  • Published : 2006.12.29

Abstract

This study analyzed the contents of vitamin C, polyphenol compounds, and evaluated the inhibitory activities against xanthine oxidase and tyrosinase of Lepista nuda extract prepared by the reflux and microwave-assisted methods. The vitamin C content of the fresh L. nude was $9.92{\pm}0.02$ mg/100 g. The content of phenolic compounds in the fresh L. nuda was $1.87{\pm}0.02\;mg/g$. The ethanol extracts prepared by reflux method and microwave-assisted method showed the highest phenol contents of $12.06{\pm}0.12\;mg/g\;and\;11.84{\pm}0.17\;mg/g$, respectively. The inhibitory rates on xanthine oxidase and tyrosinase of ethanol extracts prepared by the microwave assisted method showed the highest value of 99.78% and 30.30% at the concentration of $3,000{\mu}g/mL$. The inhibitory activities on xanthine oxidase and tyrosinase were increased depending on the extract concentration.

민자주방망이버섯(L. nuda)의 생리활성물질을 탐색하기 위하여 비타민 C, 총 폴리페놀 함량, 그리고 물과 에탄올을 용매로 환류 추출방법과 마이크로웨이브 추출방법으로 만든 각 추출물의 xanthine oxidase저해 활성 및 tyrosinase 저해 활성을 측정하였다. 각 추출물의 추출 수율은 환류 추출방법이 마이크로웨이브 추출방법보다 더 높게 나타났으며, 물 추출물보다는 에탄을 추출물의 수율이 더 높았다. 비타민 C의 함량은 $9.92{\pm}0.02$ mg/100 g이었으며, 생체의 폴리페놀 함량은 $1.87{\pm}0.02$ mg/g으로 분석되었다. 추출물의 폴리페놀 함량은 환류 에탄을 추출물(RE) $12.06{\pm}0.12$ mg/g> 마이크로웨이브 에탄올 추출물(ME) $11.84{\pm}0.17$ mg/g> 마이크로웨이브 물 추출물(MW) $9.20{\pm}0.10$ mg/g> 환류 물 추출물(RW) $8.28{\pm}0.11{\mu}g/g$ 순으로 분석 되었다. 그리고 각 추출물 중에서 $3,000{\mu}/mL$ 농도의 ME에서 xanthine oxidase 저해와 tyrosinase 저해 활성이 각각 99.78%와 30.30%로 가장 높았으며, RW가 98.30%와 20.05%로 가장 낮게 분석되었다. 이상에서 민자주방망이버섯은 항산화 작용에 효과적인 폴리페놀이 많이 함유되어 있으며, xanthine oxidase와 tyrosinase저해 활성이 높아 기능성식품 및 미용제품 등으로 이용가치가 높은 생물자원으로 사료된다.

Keywords

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