Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antiinflammatory Effects of New Chemical Compounds, HS-1580 Series (HS-1580, HS-1581, HS-1582)

신화학물질 HS-1580 유도체(HS-1580 HS-1581 HS-1582)의 항염증 효과

  • Kim, Ji-Young (Department of Biology, College of Natural Sciences, Pusan National University) ;
  • Kim, Kyun-Ha (Department of Biology, College of Natural Sciences, Pusan National University) ;
  • Suh, Hong-Suk (Department of Chemistry, College of Natural Sciences, Pusan National University) ;
  • Choi, Won-Chul (Department of Biology, College of Natural Sciences, Pusan National University)
  • 김지영 (부산대학교 자연과학대학 생물학과) ;
  • 김균하 (부산대학교 자연과학대학 생물학과) ;
  • 서홍석 (부산대학교 자연과학대학 화학과) ;
  • 최원철 (부산대학교 자연과학대학 생물학과)
  • Published : 2006.12.01
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Abstract

HS-1580 series (HS-1580, HS-1581, HS-1582) can produce anti-imflammatory effects were synthesized from the marine algae extraction in 2,3,6-tribromo-4,5-dihydroxy benzyl methyl ether (TDB). Raw 264.7 cells were pre-treated with $1{\mu}g/{\mu}l$ lipopolysaccharide (LPS) and later treated with HS-1580 series. These cells of inflammatory mediators were tested as well. Nitric oxide (NO) is related to autoimmune disease and is produced by inducible NOS (iNOS). When treated with HS-1580 series, the product of NO will reduce in a dose-dependent manner. HS-1580 series significantly inhibit the iNOS protein expression. Cyclooxygenase (COX) involves with the various physiologic events and catalyzes in prostaglandin. HS-1580 series also inhibit the COX-2 protein expression as well as pro-inflammatory cytokines production such as tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})\;and\;interluekin-1{\alpha]\;(IL-1{\beta})$. These upcoming results suggest that HS-1580 series have anti-inflammatory efforts in Raw 264.7 cells by inhibiting such as iNOS, COX-2, $TNF-{\alpha}\;and\;IL-1{\beta}$ as inflammatory mediators.

본 연구는 염증 형성과정에 있어서, 해조류로부터 항염증 효과를 나타내는 물질을 분리하여 그 유도체인 HS-1580 series (HS-1580, HS-1581, HS-1582)를 합성하였다. Nitiric oxide (NO) 생성에 있어 Raw 264.7 cells에서 lipopolysaccharide(LPS) 단독으로 처리하였을 때는 대조군에서보다 4배 이상 NO 생성이 증가하였지만, HS-1580 series를 처리하고 LPS를 처리한 군에서는 농도 의존적으로 NO 생성이 억제되었다. HS-1580 series가 NO 생성 자체를 억제함으로서 NO 함량이 감소되었는지, inducible NOS (iNOS) 단백질 발현을 억제에 기인한 것인지 알아보기 위해서 Western blot으로 조사하였다. iNOS protein 발현이 HS-1580 series에 의해서 억제되었고 HS-1580 series가 cyclooxygenase-2 (COX-2), tumor necrosis factor-a $TNF-{\alpha}$ 와 $interluekin-1{\beta}\;(IL-1{\beta})$ 생성을 농도 의존적으로 억제시켰다. 이상의 결과로 HS-1580 series가 iNOS 단백질 발현 억제에 기인한 NO 생성억제, COX-2 발현 억제 및 pro-inflammatory cytokines인 $TNF-{\alpha}$ 와 $IL-1{\beta}$ 생성을 억제하는 항염증 효과를 가짐을 알 수 있다.

Keywords

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