한국가금학회지 (Korean Journal of Poultry Science)

  • 제33권3호
  • /
  • Pages.181-188
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  • 2006
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

한국 재래 닭 고환 간질세포의 미세구조에 관한 연구

The Ultrastructure of Leydig Cells in the Testis of Korean Native Chickens

  • Jang, B.G. (Poultry Division National livestock Research Institute) ;
  • Tae, H.J. (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, C.H. (Poultry Division National livestock Research Institute) ;
  • Park, Y.J. (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Yang, H.H. (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, N.S. (Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, S.Y. (Bio-Safety Research Institute, Chonbuk National University) ;
  • Kang, H.S. (Bio-Safety Research Institute, Chonbuk National University) ;
  • Park, B.Y. (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Y.H. (School of Dentistry, Chonbuk National University) ;
  • Ahn, D.C. (Poultry Division National livestock Research Institute) ;
  • Kim, I.S. (Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University)
  • 발행 : 2006.09.01
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초록

한국 재래 닭에서 부화 후부터 성숙에 이르는 시기까지 고환 발달동안 간질세포의 미세구조를 구명하기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n=13마리/일령)의 한국 재래 닭을 이용하여 이 연구를 수행하였다. 한국 재래닭의 고환은 2.5% glutaraldehyde를 이용하여 전신관류고정하고 조직 처리 과정을 거쳐 Epon-araldite에 포매하였다. 초박절편기를 사용하여 $1{\mu}m$로 절편한 다음 methylene blue로 염색하여 일반적인 조직의 변화상을 관찰하였고 간질세포의 미세구조는 초박 절편을 제작하여 투과전자현미경으로 관찰하였다. 간질세포내에서 테스토스테론과 연관성이 있는 미세구조인 사립체, 평활형질내세망 및 지방방울의 변화에 따라 간질세포의 발달 유형을 분류하면, 1) 간질세포가 치밀하게 배열되어 있고 세포 사이의 공간이 거의 없으며 핵이 간질세포 용적의 많은 부분을 차지하고 있는 시기로 핵은 전자밀도가 높은 유형과 희박한 유형으로 나눌 수 있었고, 또한 세포질 내에는 많은 양의 지방방울, 소량의 사립체 및 평활형질내세망을 갖고 있는 시기, 2) 세포질 내에 큰 폭으로 확장된 평활형질내세망, 풍부한 사립체 및 감소된 지방방울을 갖고 있는 시기로 나눌 수 있다.

Changes in the fine structure of testicular Leydig cell from hatching to adulthood were studied in Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The objective of this study were to elucidate Leydig cell ultrastructure during testicular development. Testes of chickens were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using $1{\mu}m$ sections stained with methylene blue-azure II, qualitative and quantitative(stereological) morphological studies were performed. The ultrastructural changes of the Leydig cell were investigated by ultrathin section with the transmission electron microscope. The stages of the Leydig cell development described focus on mitochondria, endoplasmic reticulum, and lipid droplets which are involved in androgens as fullows. 1) Approaching puberty. The closely packed Leydig cells and sparse intercellular space. The nucleus occupied a large portion of the Leydig cell volume. The population of Leydig cells contained two types of cells that differed in the appearance of their nuclei which were either highly electron-opaque or relatively electron-lucid. The cytoplasm was characterized by large amounts of lipid droplets, relatively few spherical mitochondria, and sparse smooth endoplasmic reticulum. 2) Puberty to adult. The Leydig cells which display features compatible with significant androgen synthesis: large volume of cytoplasm containing extended smooth endoplasmic reticulum, abundant mitochondria, and reduction of lipid droplets.

키워드

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