DOI QR코드

DOI QR Code

Distribution of Agrobacterium tumefaciens Biovars in Jordan and Variation of Virulence

  • Al-Momani, Fouad (Department of Applied Biological Sciences, Faculty of Science, Jordan University of Science and Technology) ;
  • Albasheer, Sami (Department of Applied Biological Sciences, Faculty of Science, Jordan University of Science and Technology) ;
  • Saadoun, Ismail (Department of Applied Biological Sciences, Faculty of Science, Jordan University of Science and Technology)
  • Published : 2006.12.01

Abstract

One hundred and nine Agrobacterium isolates were recovered from 68 samples(51 plant tumor and 17 soil) that were collected from different habitats in Northern Jordan. The isolated cultures were grouped into 3 biovars based on their biochemical characteristics and biovar I, II, and III comprised a total number of 46, 41, and 22 isolates, respectively. Isolates of biovar I were obtained primarily from the diseased peach, oak and rose plants, whereas isolates of biovar II and ill were obtained mostly from apple and grape plants, respectively. Twenty-nine isolates were found to be virulent to at least one of the tested hosts such as carrots, chickpeas, garden peas and tomato plants with a response of tumor formation or tumor with roots induction. Our result suggested that A. tumefaciens strains from tumor of various plants and soil of Jordan were diverse and they have a variation in their virulence.

Keywords

References

  1. Abussaoud, M. J. I. and Al-momani, F. 1992. Microbial survey of the genus Agrobacterium in grapevine nurseries in Jordan. Arab Gulf J. Scient. Res. 10:121-131
  2. Agrios, G. N. 1978. Plant Pathology. Academic press, New York, 703pp
  3. Al-momani, F. 1987. Isolation idiotyping and characterization of the genus Agrobacterium from grape nurseries of Jordan valley and from other infected plants from different location in Jordan. M.Sc. Thesis Yarmouk University. Jordan
  4. Al-momani, F. 1991. Molecular characterization of host range determinative of Agrobacterium tumefaciens. Ph.D. Thesis University of the Punjab, Lahore, Pakistan
  5. De Cleene, M. and Deley, J. 1976. The host range of crown gall. Bot. Rev. 42:389-460 https://doi.org/10.1007/BF02860827
  6. Hooykaas, P. J. J., Alice, G. and Beijersbergen, G. M. 1994. The virulence system of Agrobacterium tumefaciens. Ann. Rev. Phytopathol. 32:157-179 https://doi.org/10.1146/annurev.py.32.090194.001105
  7. Kado, C. I. and Heskett, M. G. 1970. Selective media for isolates of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas and Xanthomonas. Phytopathology. 60:969-976 https://doi.org/10.1094/Phyto-60-969
  8. Keane, P. J., Kerr, A. and New, P. B. 1970. Crown gall of stone fruit. II Identification and nomenclature of Agrobacterium isolates. Aust. J. Bio. Sci. 23:588-598
  9. Kerr, A. and Panagopulas, C. G. 1977. Biotypes of Agrobacterium radiobacter var. tumefaciens and their biological control. Phytopath. Z. 90: 172-179 https://doi.org/10.1111/j.1439-0434.1977.tb03233.x
  10. Kersters, K., Deley J., Sne P. H. A., Sneath, and Sackin, M. 1973. Numerical taxonomic analysis of Agrobacterium. J. Gen. Microbiol. 78:227-239 https://doi.org/10.1099/00221287-78-2-227
  11. Kersters, K. and Deley, J. 1984. Genus III Agrobacterium. In: Bergey's Manuals of Systematic Bacteriology. USA. pp. 244-248
  12. King, E. O., Ward, M. K. and Raney, D. E. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301-307
  13. Ma, D., Yanofsky, M. F. Gordon, M. P. and Nester, E. W. 1987. Characterization of Agrobacterium tumefaciens strain isolated from grape vine tumor in China. App. Environ. Microbiol. 53: 13338-1343
  14. Moore, L. W. and Cooksey, D. A. 1981. Biology of Agrobacterium tumefaciens: plant interaction. In: Biology of the Rhizobiaceae, ed. by K. L. Giles and A. G. Atherly, pp. 15-46. Academic Press, New York
  15. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. New, P. B. and Kerr, A. 1971. A selective medium for Agrobacterium radiobacter biotype II. J. Appl. Bact. 34:233-236 https://doi.org/10.1111/j.1365-2672.1971.tb02281.x
  17. Panagopoulas, C. G. and Psallidas, P. G. 1973. Characteristic of Greek isolates of Agrobacterium tunefacieus. Appl. Bact. 36:233-240 https://doi.org/10.1111/j.1365-2672.1973.tb04096.x
  18. Perry, K. L. and Kado, G. I. 1982. Characteristics of the Ti- plasmid from broad host range and ecologically specific biotype II and III strains of Agrobacterium tumefaciens. J. Bacteriol. 151:343-350
  19. Sawada, H. and Ieki, H. 1992. Phenotypic characteristic of the genus Agrobacterium. Ann. Phytopathol. Soc. Jpn. 58:37-45 https://doi.org/10.3186/jjphytopath.58.37
  20. Schroth, M. N. and Moller, W. J. 1976. Crown gall controlled in glass house with a non pathogenic bacterium. Plant Disease Report, 60:275-278
  21. Sule, S. 1978. Biotypes of Agrobacterium tumefaciens in Hungary. J. Appl. Bact. 44:207-213 https://doi.org/10.1111/j.1365-2672.1978.tb00792.x
  22. Thomashow, M. F., Nutter, R., Montoya, A. L. Gordon M. P. and Nester, E. W. 1980. Integration and organization of Ti-plasmid sequences in crown gall tumor. Cell 19:729-739 https://doi.org/10.1016/S0092-8674(80)80049-3
  23. Yanofsky, M., Montoy, A., Knauf, V., Lewe, B., Gordon, M. B. and Nester, E. W. 1985. A limited host range plasmid of Agrobacterium, a molecular and genetic analysis of transferred DNA. J. Bacteriol. 163:341-348
  24. Zaenen, L., van Larabeke, N. Tenchy, H. van Mountagy, M. and Schell, J. 1974. Supercoiled circular DNA in crown gall inducing Agrobacterium strain. J. Mol. Biol. 86: 109-127 https://doi.org/10.1016/S0022-2836(74)80011-2

Cited by

  1. Susceptibility of localAgrobacterium tumefaciens strains to streptomycetes isolates from Jordan soils vol.48, pp.3, 2008, https://doi.org/10.1002/jobm.200700352