Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Quality and Sensory Characteristics of Transgenic Perilla (Perilla frutescens) Overexpressing Rot 3 gene

형질전환 들깨잎의 품질 및 관능적 특성

  • Lee, Hyeon-Suk (Division of Plant Biosciences, Kyungpook National University) ;
  • Kim, Kyung-Tae (Division of Molecular Biotechnology, College of Natural resources and Life Science, Dong-A University) ;
  • Sohn, Jae-Keun (Division of Plant Biosciences, Kyungpook National University) ;
  • Kim, Kyung-Min (Department of Environment Horticulture, College of Life Science and Natural Resources, Sangiu National University)
  • 이현숙 (경북대학교 식물생명공학부) ;
  • 김경태 (동아대학교 생명자원과학대학 분자생명과학부) ;
  • 손재근 (경북대학교 식물생명공학부) ;
  • 김경민 (상주대학교 생명자원과학대학 환경원예학과)
  • Published : 2006.06.30
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Abstract

This study was carried out to establish genetic transformation of Rot 3 gene into perilla plants and to evaluate aromatic compounds, brightness, anthocyanin contents and leaf index in Rot 3 overexpressing transgenic lines. Rot 3 transmitted successfully from T$_1$ to T$_2$ generation showing stable gene expression. It revealed that there was no difference between transgenic and non-transgenic plants in major agronomic characteristics of progeny analysis. There was not much difference in aromatic compounds and leaf brightness did not showed variations between transgenic and non-transgenic, but leaf index was distinguished, respectively.

Rot 3 유전자로 형질전환된 들깨를 이용하여 aromatic compounds, brightness, 안토시아닌 함량, leaf index를 조사하여 모본과의 품질적인 차이가 있는가를 조사하여 형질전환체의 안정성을 확보하기 위한 연구이다. Rot 3 유전자는 T1 과T2 세대에서 유전자발현이 되었으며, 형질전환이 확인된 개체는 주요 농업형질이 형질전환 되지 않은 들깨와는 차이가 없는 것으로 나타났다. 또한 aromatic compounds와 leaf brightness도 차이를 나타내지 않았다. 그러나 leaf index는 유전자의 표현을 나타내어 형질전환체와 형질전환되지 않은 들깨잎의 모양은 차이가 나타났다.

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References

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