Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Quantification of Genetically Modified Canola GT73 Using TaqMan Real-Time PCR

  • Kim, Jae-Hwan (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Song, Hee-Sung (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Dong-Hern (Division of Biosafety, National Institute of Agricultural Biotechnology) ;
  • Kim, Hae-Yeong (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2006.11.30
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Abstract

Event-specific PCR detection methods are the primary trend in genetically modified (GM) plant detection owing to their high specificity based on the flanking sequence of the exogenous integrant. Therefore, this study describes a real-time PCR system for event-specific GM canola GT73, consisting of a set of primers, TaqMan probe, and single target standard plasmid. For the specific detection of GT73 canola, the 3'-integration junction sequence between the host plant DNA and the integrated specific border was targeted. To validate the proposed method, test samples of 0, 1, 3, 5, and 10% GT73 canola were quantified. The method was also assayed with 15 different plants, and no amplification signal was observed in a real-time PCR assay with any of the species tested, other than GT73 canola.

Keywords

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