초임계이산화탄소를 이용한 탈카페인녹차 열수추출물의 포유동물 세포주를 이용한 염색체이상시험

Chromosome Aberration Test of Water Extract of Decaffeined Green Tea using Supercritical Carbon Dioxide With Mammalian Cell Line

  • 구윤창 (고려대학교 생명과학대학 식품과학부) ;
  • 이현순 (고려대학교 생명과학대학 식품과학부) ;
  • 박병규 (고려대학교 생명과학대학 식품과학부) ;
  • 김은진 (고려대학교 생명과학대학 식품과학부) ;
  • 이선주 (고려대학교 생명과학대학 식품과학부) ;
  • 김경헌 (고려대학교 생명과학대학 식품과학부) ;
  • 김영석 (이화여자대학교 공과대학 식품공학과) ;
  • 김광옥 (이화여자대학교 공과대학 식품공학과) ;
  • 정영신 ;
  • 이광원 (고려대학교 생명과학대학 식품과학부)
  • Koo, Yun-Chang (Department of Food science and Technology, College of Science and Biotechnology, Korea University) ;
  • Lee, Hyun-Sun (Department of Food science and Technology, College of Science and Biotechnology, Korea University) ;
  • Park, Byung-Gyu (Department of Food science and Technology, College of Science and Biotechnology, Korea University) ;
  • Kim, Eun-Jin (Department of Food science and Technology, College of Science and Biotechnology, Korea University) ;
  • Lee, Sun-Joo (Department of Food science and Technology, College of Science and Biotechnology, Korea University) ;
  • Kim, Kyoung-Hoen (Department of Food science and Technology, College of Science and Biotechnology, Korea University) ;
  • Kim, Young-Suk (Department of Food Science and Engineering, College of Engineering, Ewha Womans University) ;
  • Kim, Kwang-Ok (Department of Food Science and Engineering, College of Engineering, Ewha Womans University) ;
  • Chung, Young-Shin (Medvill. Co., LTD) ;
  • Lee, Kwang-Won (Department of Food science and Technology, College of Science and Biotechnology, Korea University)
  • 발행 : 2006.09.30

초록

There are $10{\sim}30%$ polyphenol and $2{\sim}4%$ caffeine in green tea. Caffeine is a kind of alkaloid containing nitrogen which cause stimulation, impatience, headache, insomnia, low birth weight infant. Because of these negative effect, decaffeined beverage came out and decaffeined coffee already have a big market since 1970s. Having proving the physiologic functions of green tea, high consumption of coffee is shifting to green tea. Because of the carcinogenic effect of the organic solvents, decaffeine processing with supercritical carbon dioxide has industrialized and have an advantage in environment-friendly and minimized flavor loss. Decaffeined green tea using supercritical carbon dioxide is considered to be safe but there are not enough study, We investigated the chromosome aberration test with mammalian cell line, CHL. When the cells were treated with 5000, 2000, 1000 ${\mu}g/ml$ and compared with the negative controls, there were no significant (P>0.05) increased chromosome aberration. Same results was observed when adding S9 mixture or not. As a result, water extract of decaffeined green tea using supercritical carbon dioxide does not induce chromosome aberration.

키워드

참고문헌

  1. Alessandra Gamberucci, Laura Konta, Angela Colucci, Roberta Giunti Judit E. Magyar, Jo zsef Mandl, Ga bor Ba nhegyi, Angelo Benedetti, Miklos Csala (2006) Green tea flavonols inhibit glucosidase II. Biochem Pharmacol, 72, 640-646 https://doi.org/10.1016/j.bcp.2006.05.016
  2. Ashihara, H. and A. Crozier (2001) Caffeine: a well known but little mentioned compound in plant science. Trends Plant Sci. 6, 407-413 https://doi.org/10.1016/S1360-1385(01)02055-6
  3. Galloway, S.M., et al (1994) Report from working group on in vitro tests for chromosomal aberration, Mutat. Res, 312, 241- 61 https://doi.org/10.1016/0165-1161(94)00012-3
  4. Hiroshi Ashihara and Alan Crozier (2001) Caffeine: a well known but little mentioned compound in plant science. TRENDS in Plant Science 6(9), 407-413 https://doi.org/10.1016/S1360-1385(01)02055-6
  5. Ishidate Jr. M. and Odashima, S. (1997) Chromosome test with 134 compounds on chinese hamster cells in vitro-a screening for chemical carcinogens. Mutat. Res, 48, 337-354
  6. Kim, B.S., Zhao, B., Kim, H.J. and Cho, M. (2000) The statistical analysis of the in vitro chromosome aberration assay using Chinese hamster ovary cells. Mutat. Res, 469, 243-52 https://doi.org/10.1016/S1383-5718(00)00086-3
  7. Kondo, K., Takase, S., Nishimoto, Y., Miyajima, H., Shiratori, O. and Miyake, Y. (2001) Genotoxicity studies of cefmatilen hydrochloride hydrate (S-1090). J Toxicol Sci. 26(1) 243-54
  8. Koyama, H. et al (1970) A new cell ine derived from new born chinese hamster lung tissue. Gann, 61, 161-167
  9. K. Ramalakshmi and B. Raghavan (1999) Caffeine in Coffee: Its Removal. Why and How? Critical Reviews in Food Science and Nutrition, 39(5), 441-456 https://doi.org/10.1080/10408699991279231
  10. Margriet Westerterp-Plantenga, Kristel Diepvens, Annemiek M.C.P. Joosen, Sonia Berube-Parent, and Angelo Tremblay (2006) Metabolic effects of spices, teas, and caffeine. Physiology & Behavior, 89, 85-91 https://doi.org/10.1016/j.physbeh.2006.01.027
  11. Nawrot, P., S. Jordan, J. Eastwood, J. Rotstein, A. Hugenholtz and M. Feeley (2003) Effects of caffeine on human health. Food Additives and Contaminants, 20, 1-30
  12. OECD (1997) OECD guideline for testing of chemicals, Section 4 : Chapter 473, In vitro mammalian chromosome aberration test. Organization for Economic Cooperation an Development, Paris, France
  13. Ping Li, Yanhui Wang, Runyu Ma, Xiaolin Zhang (2005) Separation of tea polyphenol from Green Tea Leaves by a combined CATUFM-adsorption resin process. Journal of Food Engineering, 67, 253-260 https://doi.org/10.1016/j.jfoodeng.2004.04.009
  14. Ribeiro, D.A., Marques, M.E. and Salvadori, D.M. (2006) Genotoxicity and cytotoxicity of glass ionomer cements on Chinese hamster ovary (CHO) cells. J Mater Sci Mater Med, 17(6), 495-500 https://doi.org/10.1007/s10856-006-8931-5
  15. Sakanaka, S. (2003) A novel convenient process to obtain a raw decaffeinated tea polyphenol fraction using a lignocellulose column. J. Agric. Food Chem, 51, 3140-3143 https://doi.org/10.1021/jf0208738
  16. Sera, N., Fukuhara, K., Miyata, N. and Tokiwa, H. (2001) Micronucleus induction and chromosomal aberration of 1- and 3- nitroazabenzo[a]pyrene and their N-oxides. Mutagenesis, 16(3), 183-187 https://doi.org/10.1093/mutage/16.3.183
  17. 국립환경연구원 (1998) 국립환경연구원고시 제 1998-41호 (1998 년 12월 23일) '화학물질유해성시험연구기관의 지정 등에 관한 규정'
  18. 김종태 (2000) '(주)한국차문화협회'. 제5-6호
  19. 박문호 (2003) '우리나라 녹차산업의 경쟁력 제고방안'. 농촌경제, 제26권 제1호