돈육 등급별 Hydroxyproline 함량 분석법을 이용한 육가공품의 품질 평가

Determination of Hydroxyproline Contents of Pork Meat for Quality Evaluation of Meat Products

  • 김진성 (연세대학교 생명공학과) ;
  • 김재훈 (한국원자력연구소 방사선연구원 방사선식품생명공학팀) ;
  • 박진규 (한국원자력연구소 방사선연구원 방사선식품생명공학팀) ;
  • 박재남 (한국원자력연구소 방사선연구원 방사선식품생명공학팀) ;
  • 이주운 (한국원자력연구소 방사선연구원 방사선식품생명공학팀) ;
  • 변유량 (연세대학교 생명공학과) ;
  • 변명우 (한국원자력연구소 방사선연구원 방사선식품생명공학팀)
  • Kim, Jin-Seong (Department of Biomaterials Science & Engineering, Graduate School of Yonsei University) ;
  • Kim, Jae-Hun (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Jin-Gyu (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Jae-Nam (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Yu-Ryang (Department of Biomaterials Science & Engineering, Graduate School of Yonsei University) ;
  • Byun, Myung-Woo (Radiation Food Science & Biotechnology Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 발행 : 2006.09.30

초록

본 연구는 돈육 등급과 hydroxyproline(Hyp) 함량과의 상관관계를 규명하고 최종적으로 육가공품의 품질을 평가하기 위한 방법으로의 활용 가능성을 검토하기 위해 수행되었다. 그 결과 돈육 semitendinosus(사태)와 같은 특정 부위의 Hyp 함량은 돈육 등급과 상관 관계가 없는 것으로 나타났다. 그러나, 도체로부터 분리한 fresh ham(후지) 전체 부위를 분쇄하여 시료로 사용한 경우 $A{\sim}E$등급까지 등급이 낮아질수록 Hyp 함량이 유의적으로 증가하는 것을 확인할 수 있었으며, D와 E등급을 혼합하였을 경우에도 E등급의 함량이 증가함에 따라 Hyp의 함량도 증가하는 것으로 나타났다. 또한, fresh ham(후지) 전체 부위를 원료로 하여 햄버거 패티를 제조한 후 Hyp 함량을 분석한 결과 등급이 낮아짐에 따라 Hyp 함량 역시 증가하는 것을 확인할 수 있었다. 이상의 결과를 종합하여 볼 때, 돈육 semitendinosus(사태)와 같은 특정 부위에 존재하는 Hyp의 함량은 돈육 등급과의 상관 관계를 확인할 수 없었으나, fresh ham(후지) 전체 부위를 대상으로 할 경우에는 등급에 따른 Hyp 함량과의 유의적 상관 관계가 성립될 수 있다고 판단되었다. 따라서, 육가공품의 제조시 원료용으로 많이 사용되고 있는 돈육의 경우 fresh ham(후지) 부위가 주로 사용되는 만큼 이상의 결과들은 최종적으로 육가공 제품의 품질을 평가하기 위한 기초 자료로 활용할 수 있을 것으로 기대된다.

This study was conducted to evaluate the feasibility of determination of hydroxyproline (Hyp) contents in pork meat for the quality evaluation of meat products. The relationship between the Hyp contents and pork grade was not high in porcine semitendinosus muscle. Whereas, Hyp contents of pork fresh ham and hamburger patties prepared with them were significantly increased as the decrease of pork grade. These results showed that the determination of Hyp contents might be a useful method for the quality evaluation of meat products.

키워드

참고문헌

  1. Angelo, A. J., Koohmaraie, M., Crippen, K. L., and Crouse, J. (1991) Acceleration of tenderization/inhibition of warmed-over flavor by calcium chloride-antioxidant infusion into lamb carcasses. J. Food Sci. 56, 359-363
  2. Allen, J. B. and Nicholas, D. L. (1989) Connective tissue in meat and meat products. Elsevier Applied Science, London and New York, pp. 14-25
  3. Brewer, M. S., McKeith, F., Martin, S. E., Dallmier, A. W., and Meyer, J. (1991) Sodium lactate on shelf-life, sensory, and physical characteristics of fresh pork sausage. J. Food Sci. 56, 1176-1178 https://doi.org/10.1111/j.1365-2621.1991.tb04727.x
  4. Brown, A. (1999) Understanding food: principle and preparation. Wadsworth. CA, USA, pp. 255-284
  5. Joo, S. T. and Kim, B. C. (1994). A. review of objective measuring methods to determine the pork quality. Korean J. Food Sci. Resour. 14, 90-99
  6. Judge, M. D., Aberle, E. D., Forrest, J. C., Hedrick, H. B., and Merkel, R. A. (1989) Principles of meat science. Kendall/Hunt Publishing Co., Iowa, pp. 97-224
  7. Kang, S. J. Yang, J. B., lung, I. C, and Moon, Y. H. (2001) Quality comparison of loin muscles from carcass of grade $B_2$ and D. Korean J. Food Sci. Ani. Resour. 21, 329-336
  8. Mallikatjunan, P. and Mittal, G. S. (1994) Meat quality kinetics during beef carcass chilling. J. Food Sci. 59, 291-295 https://doi.org/10.1111/j.1365-2621.1994.tb06950.x
  9. Nakai, S. and Modler, H. W. (1996) Food proteins: properties and characterization. VCH Publishers, Inc., NY. USA pp. 281-317
  10. Nikhat, J. Siddiqi, M., Abdullah, S., and Alhomida, N. (2003) Investigation into the distribution of total, free, peptide-bound, protein-bound, soluble- and insoluble. collagen hydroxyproline in various bovine tissues. J. Biochern. Mol. Biol. 36, 154-158
  11. Nold, R. A., Romans, J. R., Costello, W. J. and Libal, G. W. (1999) Characterization of muscles from boars, barrows, and gilts slaughtered at 100 or 110 Kilograms: Differences in fat, moisture, color, water-holding capacity, and collagen. J. Anim. Sci. 77, 1746-1754 https://doi.org/10.2527/1999.7771746x
  12. Reddy, K. and Enwemeka, C S. (1996) A simplified method for the analysis of hydroxyproline in biological tissues. Clin. Biochem. 29, 225-229 https://doi.org/10.1016/0009-9120(96)00003-6
  13. Rusman, Soeparno, Setitono, and Suzuki, A. (2003) Characteristics of Biceps femoris and Longissimus thoracis muscles of five cattle breeds grown in a feedlot system. J. Anim. Sci. 74, 59-65 https://doi.org/10.1046/j.1344-3941.2003.00087.x
  14. SAS Institute, Inc. (1998) SAS user's guide. SAS Institute Inc, Cary, NC
  15. Savell, J. W., Cross, H. R., Francis, J. J., Wise, J. W., Hale, D. S., WkIkes, D. L., and Smith, G. C. (1989) National consumer retail beef study: interaction of trim level, price and grade on consumer acceptance of bed steaks and roasts. J. Food Qual. 12, 251-255 https://doi.org/10.1111/j.1745-4557.1989.tb00328.x
  16. Takahashi, K. (1996) Structural weakening of skeletal muscle tissue during post-mortem ageing of meat: The non-enzymatic mechanism of meat tenderization. Meat Sci. 43, 67-80 https://doi.org/10.1016/0309-1740(96)00056-3
  17. Whipple, G., Koohmaraie, M., Dikeman, M. E., Crouse, J. D., Hunt, M. C, and Klemm, R. D. (1990) Evaluation of attributes that affect longissimuss muscle tenderness in Bos taurus and Bos indicus cattle. J. Anim. Sci. 68, 2716-2721 https://doi.org/10.2527/1990.6892716x
  18. Winger, R. J. and Fennema, O. (1976) Tenderness and water holding properties of beef muscle as influenced by freezing and subsequent storage at -$3^{\circ}C$ and $15^{\circ}C$. J. Food Sci. 41, 1433-1439 https://doi.org/10.1111/j.1365-2621.1976.tb01189.x