Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Characterization of Encephalomyocarditis Virus Isolated from Aborted Swine Fetus in Korea

  • Song, Min-Suk (College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Joo, Young-Ho (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Eun-Ho (College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Shin, Jin-Young (College of Medicine and Medical Research Institute, Chungbuk National University) ;
  • Kim, Chul-Jung (College of Veterinary Medicine, Chungnam National University) ;
  • Shin, Kwang-Soon (College of Veterinary Medicine, Chungnam National University) ;
  • Sung, Moon-Hee (Department of Bio and Nano Chemistry, College of Natural Sciences, Kookmin University) ;
  • Choi, Young-Ki (College of Medicine and Medical Research Institute, Chungbuk National University)
  • Published : 2006.10.31
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Abstract

An encephalomyocarditis virus (EMCV-CBNU) was isolated from an aborted swine fetus in October 2005. To investigate the genetic origin and virulence of the EMCV-CBNU strain, we determined the complete sequence of the virus and tested its virulence in mice. Genetic characterization revealed that the RNA genome was composed of 7,713 nucleotides with a single open reading frame (2,292 amino acids), coding 12 proteins. The EMCV-CBNU had the shortest poly(C) tract, consisting of 10 C's ($C_{10}$), compared with all the other EMCV strains reported in GenBank. Amino acid and phylogenetic analyses showed that EMCV-CBNU had the highest genetic identity with strain 2887A (99.7%), which was originally isolated from a fetus in a pig breeding farm that had a history of reproductive failure. Because rodents are the natural host of EMCV, we investigated the virulence of EMCV-CBNU in mice. Surprisingly, all mice inoculated with more than $1{\times}10^2\;TCID_{50}/0.1ml$ of EMCV-CBNU showed symptoms of hind limb paralysis and eventually died during 3 and 8 days postinoculation (DPI). Furthermore, when we inoculated the virus into pregnant mice, all dams and their fetuses died in 6 DPI. This is the first report on a full genomic analysis of swine EMCV in Korea, which exhibits high virulence in mice.

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References

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