Advances in Traditional Medicine

  • 제6권2호
  • /
  • Pages.86-95
  • /
  • 2006
  • /
  • 2662-4052(pISSN)
  • /
  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
권호 표지
DOI QR코드

DOI QR Code

Spermatogenic index and hormonal profile in the rats received chromatographic fractions of ethanol extract of Crotalaria juncea L. seeds

  • 발행 : 2006.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The ethanol extract of the Crotalaria juncea seeds, which showed promising antispermatogenic and antiandrogenic activities in albino mice, was taken up further for the isolation of the active fractions present in it. Two fractions that were obtained from thin layer chromatography were subjected for testing to know their antispermatogenic and antiandrogenic activities. After preliminary trials the fraction I showed maximum antifertility activity at the dose level of 200 mg/kg body weight when administered orally to the rats for 50 days. The fraction I was found to affect spermatogenesis as well as the endocrine functions of the testis as indicated by gravimetric, histopathological and biochemical changes. Further this fraction has caused degenerative changes in the seminiferous tubules and Leydig cells of the testis. The accessory reproductive organs like epididymis, seminal vesicles, vas deferens, prostrate, Cowper's gland and Levator Ani muscle showed significant malfunction. Cauda epididymal sperm count and sperm motility were reduced significantly. The treatment has also resulted in increase in the cholesterol level and alkaline phosphatase activity, and decrease in protein, glycogen, sialic acid contents and acid phosphatase activity in testis. It is noteworthy that RIA studies have shown significant reduction in serum FSH, LH and testosterone. Scanning electron microscopic observations revealed abnormalities in sperm structure.

키워드

참고문헌

  1. Abercrombie M. (1946). Estimation of nuclear population from microtome sections Anat. Rec. 94, 238-243.
  2. Bessey OA, Brick NJ, Lowry OH, Brick NJ. (1946) A method for rapid determination of acid and alkaline phosphatase with 5 cu. mm. of serum. J. Biol. Chem. 164, 321.
  3. Carrol NV, Langelly RW, Row RH. (1956) Glycogen determination in liver and muscle by use of anthrone reagent. J. Biol. Chem. 20, 583-593.
  4. Chemes HE, Dym M, Raj HGM. (1979) The role of gonadotrophins and testosterone on initiation of spermatogenesis in the immature rat. Biol. Reprod. 21, 241-248. https://doi.org/10.1095/biolreprod21.1.241
  5. Chinoy NJ, Reddy VVIC, Michael M. (1994) Beneficial effects of ascorbic acid and calcium on reproductive functions of sodium fluoride treated male rats. Fluoride 27, 21-29
  6. Chinoy NJ, Seethalakshmi L, Buch RP, Sanjeevan AG. (1979) Studies on sperm maturation. In: Recent Advances in Reproduction and Regulation of Fertility. ed., G. P. Talwar, Elsevier / North Holland, Amsterdam, pp. 259-265.
  7. Chinoy NJ, Sequeira E. (1989) Fluoride induced biochemical changes in reproductive organs of male mice. Fluoride 22, 78-85.
  8. Christensen AK. (1975) Leydig cells In: Greep, R.O and Astwood, E.B., editors. Handbook of Physiology. Washington, Amer. Physiol. Soc., pp. 57-94.
  9. Connel GM, Eik-Nes KB. (1968) Testosterone production by rabbit testis slices. Steroids, 12, 507. https://doi.org/10.1016/S0039-128X(68)80114-X
  10. Dabrowski K, Ciereszko A. (1996) Ascorbic acid protects against male infertility in a teleost fish. Experentia 52, 97-100. https://doi.org/10.1007/BF01923351
  11. Deb C, Boral MC, Sarkar C. (1964) Measurement of hepatic parenchymal cell and nuclear volume in different classes of vertebrates. Anat. Rec. 148, 449.
  12. Dixit VP, Gupta CL, Bhargava SK, Sandhu JS. (1979) Long-term effect of metopiron (SU-4885 CIBA) on the testicular function of dogs. Folia Biol. (Krakow) 27, 277-285.
  13. Dorrington JH, Armstrong DT. (1975) Follicle stimulating hormone stimulates estradiol-17b synthesis in cultured sertoli cells. Proc. Natl. Acad. Sci. U.S.A. 72, 2677. https://doi.org/10.1073/pnas.72.7.2677
  14. Flickinger CJ. (1981) Regional difference in synthesis, intracellular transport and secretion of protein in the mouse epididymis. Biol. Reprod. 25, 871-883. https://doi.org/10.1095/biolreprod25.4.871
  15. Gaddam P, Glover JD. (1985) Some reactions of rabbit spermatozoa to ligation of the epididymis. J. Reprod. Fertil. 9, 119.
  16. Gerard H, Gerard A, EnNya A, Gueant JL. (1994) Spermatogenic cells do internalize sertoli androgenbinding protein: a transmission electron microscopy autoradiographic study in the rat. Endocrinology 134, 1515-1527. https://doi.org/10.1210/en.134.3.1515
  17. Ghosh S, Bartke A, Grasso P, Reichert LE Jr, Rassell LD. (1992) Structural manifestation of the rat sertoli cell to hypophysectomy: a correlative morphometric and endocrine study. Endocrinology 131, 85.
  18. Gupta RS, Kumar P, Dixit VP, Dobhal MP. (2000) Antifertility studies of the root extract Barleria prionities Linn in male albino rats with special reference to testicular cell population dynamics. J. Ethnopharmacol. 75, 5-12. https://doi.org/10.1016/S0378-8741(00)00362-7
  19. Gupta RS, Kumar P, Dixit VP, Dobhal MP. (2000) Antifertility studies of the root extract of the Barleria Prionitis Linn.in male albino rats with special reference to testicular cell population dynamics. J. Ethnopharmacol. 70, 111-117. https://doi.org/10.1016/S0378-8741(99)00150-6
  20. Gurr E. (1962) Staining Animal Tissues. Practical and Theoretical Leonard Hill Limited. London, pp. 233.
  21. Hall PF. (1994) Testicular steroid synthesis: Organization and regulation. In: Physiology of Reproduction. (Eds: Knobil E, Neill JD), Raven Press, New York, 1, 1335.
  22. Haneji T, Maekawa M, Nishimune Y. (1984) Vitamin A and FSH synergistically induce differentiation of type A spermatogonia in adult male mouse cryptorchid testis in vitro. Endocrinology 114, 801. https://doi.org/10.1210/endo-114-3-801
  23. Hariharan S. (1980) Laboratory Animals Information Service Centre, pp. 9-11, New ICMR, Hyderabad.
  24. Holt JA, Kelch RP, Payne AH. (1973) In vitro effect of rat LH and FSH and hCG on androgen (A) and estrogen (E) release from decapsulating rat testes. Proc. Annu. Meet. Soc. 6, 58.
  25. Johnson BJ, Ewing. (1971) Follicle stimulating hormone and the regulation of testosterone secretion in rabbit testes. Science 173, 635. https://doi.org/10.1126/science.173.3997.635
  26. Kamboj VP, Dhawan BN. (1989) Fertility regulating plants on Indian scene: an update In: Contraceptive Research Today and Tomorrow (eds. Toteja GS, Mokkapati S, Singh BK, Sharma RS, Saxena BN), pp. 115, ICMR, New Delhi.
  27. Kempinas WG, Lamano Carvalho JL. (1987) A method for estimation the concentration of spermatozoa in the rat cauda epididymis. Lab. Anim., pp. 154.
  28. Kirk JE. (1962) Variations with age in the tissue content of vitamins and hormones. Vit. Horm. 20, 67-139. https://doi.org/10.1016/S0083-6729(08)60716-5
  29. Latchoumycandane C, Gupta SK, Mathur PP. (1997) Inhibitory effects of hypothyroidism on the testicular functions of postnatal rats. Bimed. Lett. 56, 171-177.
  30. Latchoumycandane C, Mathur PP. (1999) Effects of hyperthyroidism on the physiological status of pubertal rat testis. Biomed. Lett. 59, 33-41.
  31. Lowry OHN, Rosenbrough J, Farr NL, Randall RJ. (1951) Protein measurement with folic phenol reagent. J. Biol. Chem. 193, 265-275.
  32. Males JL, Turkington RW. (1971) Hormonal control of lysosomal enzymes during spermatogenesis in the rat. Endocrinology 88, 579-589. https://doi.org/10.1210/endo-88-3-579
  33. Mathur PP, Chattopadhyay S. (1982) Involvement of lysosomal enzymes in flutamide - induced stimulation of rat testis. Andrologia 14, 171-176. https://doi.org/10.1111/j.1439-0272.1982.tb03120.x
  34. Mohri H, Sutur DAE, Brown-Woedman PDE, White IG, Ridley PD. (1978) Nature 255, 75. https://doi.org/10.1038/255075a0
  35. Nair VCO, Alao TO, Okogun JI, Raji Y, Balarinwa AF, Nduka FW. (1995) Antifertility activity of Quassia amara. Quassin inhibits the steroidogenesis in rat Leydig cells in vitro. Planta Med. 61, 180. https://doi.org/10.1055/s-2006-958044
  36. Peters JP, Vanslyke DD. (1946) Quantitative Clinical Chemistry, Vol I, Williams and Wilkins, Baltimore.
  37. Pudney J. (1986) Fine structural changes in sertoli and Leydig cells during the reproductive cycle of the ground squirrel, Citellus lateralis. J. Reprod. Fertil. 77, 37-49. https://doi.org/10.1530/jrf.0.0770037
  38. Reddy CM, Murthy DRK, Patil SB. (1997) Antispermatogenic and androgenic activities of various extracts of Hibiscus rosa Sinensis in albino mice. Indian J. Exp. Biol. 35, 1170-1174.
  39. Ritzen EM, Biotani C, Parvinen M, French FS, Feldman M. (1982) Stage-dependent secretion of ABP by rat seminiferous tubules. Mol. Cell. Endocrinol. 25, 25-34. https://doi.org/10.1016/0303-7207(82)90166-6
  40. Roe JH, Kuether CA. (1943) The determination of ascorbic acid in whole blood and uring through the 2-4 dinitrophenyl hydrazine derivative of dehydroascorbic acid. J. Biol. Chem. 147, 399-407.
  41. Russel LD, Alger LE, Nequin LG. (1987) Hormonal control of puberty spermatogenesis. Endocrinology 120, 1615. https://doi.org/10.1210/endo-120-4-1615
  42. Sarkar HBD. (1996) Principle of Vertebrate Reproductive Biology, Published by Ganapathy Graphics, Madras 1, 130.
  43. Sarkar SN, Majumdar AC, Chattopadhyay SK. (1997) Effect of isoproturon on male reproductive system: clinical, histological and histoenzymological studies in rats. Indian J. Exp. Biol. 35, 133-138.
  44. Vijaykumar B. (2005) Studies on the antifertility activities of some natural products. Thesis submitted to Gulbarga University.
  45. Viajykumar B, Sangamma A, Sharanabasappa A, Saraswati BP. (2004) Antispermatogenic and hormonal effects of Crotalaria juncea L. seed extracts in male mice. Asian J. Androl. 6, 67-71.
  46. Warren L. (1959) The thiobarbituric assay of sialic acids. J. Biol. Chem. 243, 1971.